Iingcali zelabhoratri zinokuchitha iiyure ngosuku ngalunye zibambe i-micropipette, kunye nokuphucula ukusebenza kakuhle kwemibhobho kunye nokuqinisekisa iziphumo ezithembekileyo kudla ngokuba ngumngeni.Ukukhetha i-micropipette efanelekileyo kuyo nasiphi na isicelo singundoqo kwimpumelelo yomsebenzi webhubhoratri; akuqinisekisi kuphela ukusebenza kwanoma yimuphi umfuniselo, kodwa kwandisa ukusebenza kakuhle.Ukuqonda iimfuno zemibhobho yomsebenzi kuvumela abasebenzisi ukuba bakhethe iipayipi ezichanekileyo kunye neziphindaphindayo, kodwa kukho ezinye izinto ezininzi ezimele ziqwalaselwe ukuphucula iziphumo zepayipi kunye nokuqinisekisa impumelelo yovavanyo.
Ukuthetha ngokubanzi, ulwelo luwela kwiindidi ezintathu eziphambili: ezinamanzi, i-viscous, kunye ne-volatile.Ininzi yolwelo isekelwe kumanzi, okwenza i-pipette yokufuduka komoya ibe yinto yokuqala yokukhetha abaninzi.Ngelixa uninzi lwe-liquid lusebenza kakuhle ngolu hlobo lwe-pipette, iipayipi ze-volumetric kufuneka zikhethwe xa zisebenza ngolwelo olubonakalayo kakhulu okanye oluguquguqukayo.Umahluko phakathi kwezi ntlobo ze-pipette ezichanekileyo zibonakaliswe kwi-Figurt echanekileyo yokusetyenziswa kwe-figure. uhlobo - ukufumana iziphumo ezibalaseleyo.
Iiparamitha ezimbini ezibaluleke kakhulu ezichaphazela iziphumo zombhobho zichanekile kunye nokuchaneka (Umfanekiso 2) .Ukufezekisa ukuchaneka okuphezulu kwepayipi, ukuchaneka, kunye nokuthembeka, iikhrayitheriya ezininzi kufuneka zigcinwe engqondweni.Njengomthetho wesithupha, umsebenzisi kufuneka ahlale ekhetha i-pipette encinci enokusingatha umthamo wokudlulisa ofunwayo.Oku kubalulekile njengoko ukuchaneka kuyancipha njengoko i-volume ye-pipette iyancipha njengoko isethi ye-pipette isondela kwi-50. µl nge-5,000 µl pipette, iziphumo zinokuba zimbi. Iziphumo ezingcono zinokufunyanwa nge-300 µl pipettes, ngelixa i-50 µl pipettes ibonelela ngezona ziphumo zingcono. Ukongeza, ivolyum iseti yeepayipi zezandla zesintu zinokutshintsha ngexesha lokufakela iipayipi ngenxa yokujikeleziswa kwemibhobho ngempazamo ngelixa uhlengahlengiso lwemibhobho yohlengahlengiso lweloti luthintele uyilo lwemibhobho yokufaka i-loti. ukuqinisekisa ngakumbi ukuchaneka.Ukulinganisa yenye into ebalulekileyo enceda ukuqinisekisa iziphumo ezithembekileyo ngokubonisa ukuchaneka nokuchaneka kwepayipi.Le nkqubo kufuneka ibe lula kumsebenzisi; umzekelo, ezinye iipayipi ze-elektroniki zinokubeka izikhumbuzo zokulinganisa, okanye zigcine imbali yokulinganisa.Ayiyo nje i-pipettes ekufuneka iqwalaselwe.Ukuba incam ye-pipette iyancipha, ivuza, okanye iwe, inokubangela iingxaki ezahlukeneyo.Le ngxaki eqhelekileyo kwilebhu idla ngokubangelwa ukusetyenziswa kweengcebiso ze-pipette zenjongo jikelele, ezihlala zifuna "ukucofa." indawo engafanelekanga, okanye ibangele ukuba i-tip iwele kwi-pipette ngokupheleleyo.Ukukhetha i-micropipette ephezulu eyenziwe ngeengcebiso ezithile kuqinisekisa uxhulumaniso olukhuselekileyo, ukubonelela ngezinga eliphezulu lokuthembeka kunye neziphumo ezingcono.Ukongezelela, into elula njenge-coding color-coding pipettes kunye neengcebiso nazo zinokunceda abasebenzisi baqinisekise ukuba iingcebiso ezichanekileyo zikhethwa kwiipayipi zabo.
Kwindawo ephezulu, kubalulekile ukuba usebenze ngokufanelekileyo ngelixa ugcina ukuthembeka kunye nokuhambelana kwenkqubo yepayipi.Kukho iindlela ezininzi zokuphucula ukusebenza kakuhle kwepayipi, kubandakanywa ukusetyenziswa kwe-multichannel kunye / okanye i-pipettes ye-elektroniki.Ezi zixhobo eziguquguqukayo zihlala zibonelela ngeendlela ezininzi ezahlukeneyo zombhobho-ezifana ne-reverse pipetting, variable dispensing, i-projected more processed, i-applicationed processed, i-application-programmed processed. ezifana nokuphindaphinda ngokuphindaphindiweyo kulungele ukukhupha i-aliquots ezininzi zomthamo ofanayo ngaphandle kokuzalisa kwakhona i-tip.Ukusebenzisa i-pipettes enye-channel ukudlulisa iisampulu phakathi kweefomathi ezahlukeneyo ze-labware inokukhawuleza ibe yinto edinayo kwaye iphosakele.Iipayipi ezininzi ze-Multichannel zivumela ukuhanjiswa kweisampulu ezininzi ngexesha elinye ngokuqhwanyaza kweso.Akukuphela nje ukuba oku kwandisa i-pipetipe esebenzayo (iphinde incede i-retraining ye-pipetime). iipipettes bade babe nekhono lokwahluka izithuba zengcebiso ngexesha lokubhobhoza, ukuvumela ukuhanjiswa okuhambelanayo kweesampulu ezininzi phakathi kobukhulu obuhlukeneyo be-labware kunye neefomathi, ukonga iiyure zexesha (Umfanekiso 3).
Iingcali zaselabhoratri zihlala zichitha iiyure ngosuku ngepayipi.Oku kunokubangela ukungonwabi kwaye, kwiimeko ezimandundu, nkqu nokulimala kwesandla okanye ingalo.Elona cebiso lilungileyo lokuphepha ezi ngozi zinokubakho kukunciphisa ubungakanani bexesha obambe i-pipette kwixesha elifutshane elinokwenzeka.Ukongezelela koku, abasebenzisi kufuneka bakhethe i-micropipette ekhaphukhaphu kwaye elungelelaniswe kakuhle enobunzima kwiziko lozinzo olungcono, kwaye kwisandla sasekunene kufuneka ube nesandla esifanelekileyo sokuzinza kakuhle. idizayini yokubamba, kwaye ulungelelanise ivolumu ngokukhululeka kwaye ngokukhawuleza kunokwenzeka ukuphepha ukunyakaza okungadingekile.Kwakhona, iingcebiso zibalulekile, njengoko ukulayishwa kweengcebiso kunye ne-ejection kudla ngokufuna amandla angaphezu kombhobho kwaye kukho umngcipheko wokulimala, ngokukodwa kwizicwangciso eziphezulu.
Xa ukhetha i-micropipette efanelekileyo kwisicelo sakho, kubalulekile ukujonga zonke iinkalo zokuhamba komsebenzi wakho.Ngokuqwalasela i-pipette, iimpawu zayo, uhlobo kunye nomthamo we-liquid pipetted, kunye neengcebiso ezisetyenzisiweyo, izazinzulu zinokuqinisekisa iziphumo ezichanekileyo, ezichanekileyo kunye nezithembekileyo ngelixa zigcina imveliso kunye nokunciphisa umngcipheko wokulimala.
Kolu shicilelo, ukubuyiswa kwabahlalutyi abasisiseko kuhlolwa yi-HPLC-MS kusetyenziswa imowudi edibeneyo yokutshintshiselana nge-cation exchange SPE microplates. Izibonelelo ze-SEC-MALLS kwizicelo ze-biopharmaceutical...
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Ixesha lokuposa: Jun-10-2022