Iingcali zelabhoratri zinokuchitha iiyure ngosuku ngalunye zibambe i-micropipette, kunye nokuphucula ukusebenza kakuhle kwemibhobho kunye nokuqinisekisa iziphumo ezithembekileyo kudla ngokuba ngumngeni.Ukukhetha i-micropipette efanelekileyo kuyo nasiphi na isicelo singundoqo kwimpumelelo yomsebenzi webhubhoratri; ayiqinisekisi kuphela ukusebenza kwawo nawuphi na umfuniselo, kodwa ikwanyusa ukusebenza kakuhle.Ukuqonda iimfuno zokuhamba kombhobho kwenza abasebenzisi bakwazi ukukhetha iipipettes ezichanekileyo neziphindaphindwayo, kodwa kukho ezinye izinto ezininzi ekufuneka ziqwalaselwe ukuze kuphuculwe iziphumo zemibhobho kunye nokuqinisekisa impumelelo. imifuniselo.
Ukuthetha ngokubanzi, ulwelo luwela kwiindidi ezintathu eziphambili: ezinamanzi, i-viscous, kunye ne-volatile.Uninzi lwe-liquids lusekelwe kumanzi, okwenza i-pipettes yokufuduka komoya ibe yinto yokuqala yokukhetha abaninzi.Ngeli xesha ulwelo oluninzi lusebenza kakuhle ngolu hlobo lwe-pipette, i-pipettes ye-volumetric kufuneka ikhethwe xa ukusebenza nge-viscous kakhulu okanye ulwelo oluguquguqukayo.Umahluko phakathi kwezi ntlobo ze-pipette ziboniswe kuMfanekiso 1. Kwakhona kubalulekile ukusebenzisa ubuchule obuchanekileyo bokufaka imibhobho - kungakhathaliseki ukuba lulwelo. uhlobo - ukufumana iziphumo ezibalaseleyo.
Iiparamitha ezimbini ezibaluleke kakhulu ezichaphazela iziphumo zemibhobho zichanekile kwaye zichanekile (Umfanekiso 2) .Ukufezekisa ukuchaneka okuphezulu kwepayipi, ukuchaneka, kunye nokuthembeka, imigaqo emininzi kufuneka igcinwe engqondweni.Njengomthetho wesithupha, umsebenzisi kufuneka ahlale ekhetha i-pipette encinci. enokusingatha umthamo wogqithiselo olufunwayo.Oku kubalulekile njengoko ukuchaneka kuncipha njengoko isethi yevolumu isondela kumthamo omncinci wepayipi.Umzekelo, ukuba ukhupha i-50 I-µl nge-5,000 µl pipette, iziphumo zinokuba zimbi. Iziphumo ezingcono zinokufunyanwa nge-300 µl pipettes, ngelixa i-50 µl pipette inika iziphumo ezingcono. Yiyo loo nto abanye abavelisi be-pipette baye baphuhlisa ukuyila kohlengahlengiso lokutshixa umthamo ukuthintela utshintsho olungalindelekanga ngelixa ubetha umbhobho. ukuqinisekisa ngakumbi ukuchaneka.Ukulinganisa yenye into ebalulekileyo enceda ukuqinisekisa iziphumo ezithembekileyo ngokubonisa ukuchaneka nokuchaneka kwepayipi.Le nkqubo kufuneka ibe lula kumsebenzisi; umzekelo, ezinye iipayipi ze-elektroniki zinokuseta izikhumbuzi zokulinganisa, okanye zigcine imbali yokulinganisa.Ayiyo nje i-pipettes ekufuneka iqwalaselwe.Ukuba incam yepipette ikhululekile, ivuza, okanye iwe, inokubangela iingxaki ezahlukeneyo.Le ngxaki ixhaphakileyo kwilebhu. isoloko ibangelwa kukusetyenziswa kweengcebiso ze-pipette zenjongo jikelele, ezihlala zifuna "ukucofa." Le nkqubo yolula isiphelo sencam yepipette kwaye inokubangela ukuba incam ivuze okanye ivuze. indawo engafanelekanga, okanye ibangele ukuba i-tip iwele kwi-pipette ngokupheleleyo.Ukukhetha i-micropipette ephezulu eyenziwe ngeengcebiso ezithile kuqinisekisa uxhulumaniso olukhuselekileyo, ukubonelela ngezinga eliphezulu lokuthembeka kunye neziphumo ezingcono.Ukongezelela, into elula njenge-coding-coding pipettes kunye neengcebiso zinokunceda abasebenzisi baqinisekise ukuba iingcebiso ezichanekileyo zikhethwa kwiipipettes zabo.
Kwindawo ephezulu, kubalulekile ukuba usebenze ngokusemandleni ngelixa ugcina ukuthembeka kunye nokuhambelana kwenkqubo yokubhobhoza.Kukho iindlela ezininzi zokuphucula ukusebenza kakuhle kwepayipi, kubandakanywa nokusetyenziswa kwe-multichannel kunye / okanye i-pipettes ye-elektroniki.Ezi zixhobo eziguquguqukayo. zihlala zibonelela ngeendlela ezininzi ezahlukeneyo zokubeka imibhobho-efana nokubuyela umva kwemibhobho, ukwabiwa okuguquguqukayo, uthotho lweenkqubo ezicwangcisiweyo, nokunye-ukwenza lula inkqubo.Umzekelo, iinkqubo ezifana nokuhanjiswa okuphindaphindiweyo kulungele ukukhupha i-aliquots ezininzi zomthamo ofanayo ngaphandle kokuzalisa kwakhona i-tip.Ukusebenzisa i-pipettes ye-channel enye ukudlulisa iisampulu phakathi kweefomathi ezahlukeneyo ze-labware inokukhawuleza ibe yinto edinayo kwaye iphosakele.Iipayipi ezininzi ze-Multichannel zivumela ukudlulisa iisampuli ezininzi ngexesha elinye ngokuqhwanyaza kweliso.Akukuphela nje oku kwandisa ukusebenza kakuhle, kukwanceda ukuthintela iimpazamo zemibhobho kunye nokulimala okuphindaphindiweyo (RSI) .Abanye iipipettes bade babe nekhono lokwahluka izithuba zengcebiso ngexesha lokubhobhoza, ukuvumela ukuhanjiswa okuhambelanayo kweesampulu ezininzi phakathi kobukhulu obuhlukeneyo be-labware kunye neefomathi, ukonga iiyure zexesha (Umfanekiso 3).
Iingcali zelabhoratri zihlala zichitha iiyure ngosuku nge-pipetting.Oku kunokubangela ukuphazamiseka kwaye, kwiimeko ezinzima kakhulu, nokuba yisandla okanye ukulimala kwengalo.Ingcebiso efanelekileyo yokuphepha le mingcipheko enokubakho kukunciphisa ixesha lokubamba i-pipette kwixesha elifutshane. .Ukongezelela koku, abasebenzisi kufuneka bakhethe i-micropipette ekhaphukhaphu kunye nokulungelelaniswa kakuhle kunye nobunzima phakathi kwiziko lokuzinza okungcono. idizayini yokubamba, kwaye ulungelelanise ivolumu ngokukhululeka kwaye ngokukhawuleza kunokwenzeka ukuphepha ukunyakaza okungadingekile.Kwakhona, iingcebiso zibalulekile, njengoko ukulayishwa kweengcebiso kunye ne-ejection kudla ngokufuna amandla angaphezulu kunombhobho kwaye kukho umngcipheko wokulimala, ngakumbi kwimimiselo ephezulu. .Iingcebiso zePipette kufuneka zingene endaweni ngamandla amancinci, zinikeze uqhagamshelwano olukhuselekileyo, kwaye kube lula ngokulinganayo ukukhupha.
Xa ukhetha i-micropipette efanelekileyo kwisicelo sakho, kubalulekile ukujonga zonke iinkalo zokuhamba komsebenzi wakho.Ngokuqwalasela i-pipette, iimpawu zayo, uhlobo kunye nomthamo wolwelo olufakwe kwipayipi, kunye neengcebiso ezisetyenzisiweyo, izazinzulu zinokuqinisekisa ukuba zichanekile, zichanekile kwaye zithembekile. iziphumo ngelixa ugcina imveliso kunye nokunciphisa umngcipheko wokulimala.
Kolu shicilelo, ukubuyiswa kwabahlalutyi abasisiseko kuhlolwa yi-HPLC-MS kusetyenziswa imowudi edibeneyo yokutshintshiselana nge-cation exchange SPE microplates. Izibonelelo ze-SEC-MALLS kwizicelo ze-biopharmaceutical...
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Ixesha lokuposa: Jun-10-2022