Iingcali zelabhoratri zinokuchitha iiyure ngosuku ngalunye zibambe i-micropipette, kunye nokuphucula ukusebenza kakuhle kwemibhobho kunye nokuqinisekisa iziphumo ezithembekileyo kudla ngokuba ngumngeni.Ukukhetha i-micropipette efanelekileyo kuyo nasiphi na isicelo singundoqo kwimpumelelo yomsebenzi webhubhoratri; ayiqinisekisi kuphela ukusebenza kwawo nawuphi na umfuniselo, kodwa ikwanyusa ukusebenza kakuhle.Ukuqonda iimfuno zokuhamba kombhobho kwenza abasebenzisi bakwazi ukukhetha iipipettes ezichanekileyo neziphindaphindwayo, kodwa kukho ezinye izinto ezininzi ekufuneka ziqwalaselwe ukuze kuphuculwe iziphumo zemibhobho kunye nokuqinisekisa impumelelo. imifuniselo.
Ukuthetha ngokubanzi, ulwelo luwela kwiindidi ezintathu eziphambili: ezinamanzi, i-viscous, kunye ne-volatile.Uninzi lwe-liquids lusekelwe kumanzi, okwenza i-pipettes yokufuduka komoya ibe yinto yokuqala yokukhetha abaninzi.Ngeli xesha ulwelo oluninzi lusebenza kakuhle ngolu hlobo lwe-pipette, i-pipettes ye-volumetric kufuneka ikhethwe xa ukusebenza nge-viscous kakhulu okanye ulwelo oluguquguqukayo.Ukwahluka phakathi kwezi ntlobo ze-pipette kuboniswe kuMzobo 1. Kwakhona kubalulekile ukusebenzisa ubuchule obuchanekileyo bokufaka umbhobho - kungakhathaliseki ukuba luhlobo luni lwe-liquid - kwiziphumo ezigqwesileyo.
Iiparamitha ezimbini ezibaluleke kakhulu ezichaphazela iziphumo zemibhobho zichanekile kwaye zichanekile (Umfanekiso 2) .Ukufezekisa ukuchaneka okuphezulu kwepayipi, ukuchaneka, kunye nokuthembeka, imigaqo emininzi kufuneka igcinwe engqondweni.Njengomthetho wesithupha, umsebenzisi kufuneka ahlale ekhetha i-pipette encinci. enokusingatha umthamo wogqithiselo olufunwayo. Oku kubalulekile njengoko ukuchaneka kuhla njengoko umthamo omiselweyo usondela kumthamo ophantsi wepipette.Umzekelo, ukuba ukhupha i-50 µl nge-5,000 µl pipette, iziphumo zinokuba zimbi. Iziphumo ezingcono zingaba efunyenwe ngeepipettes ezingama-300 µl, ngelixa i-50 µl pipettes ibonelela ngezona ziphumo zingcono. Ukongeza, umthamo oseti kwiipayipi zezandla zesintu unokutshintsha ngexesha lokubhobhoza ngenxa yokujikeleza ngempazamo kweplunger. utshintsho olungabonakaliyo ngelixa i-pipetting iqinisekisa ngakumbi ukuchaneka.Ukulinganisa enye into ebalulekileyo enceda ukuqinisekisa iziphumo ezithembekileyo ngokubonisa ukuchaneka nokuchaneka kwe-pipette.Le nkqubo kufuneka ibe lula kumsebenzisi; umzekelo, ezinye iipayipi ze-elektroniki zinokuseta izikhumbuzi zokulinganisa, okanye zigcine imbali yokulinganisa.Ayiyo nje i-pipettes ekufuneka iqwalaselwe.Ukuba incam yepipette iyakhululeka, ivuze, okanye iwe, inokubangela iingxaki ezahlukeneyo.Le ngxaki ixhaphakileyo kwilebhu. Idla ngokubangelwa kukusetyenziswa kweengcebiso ze-pipette zenjongo jikelele, ezihlala zifuna "ukucofa."Le nkqubo yolula isiphelo sencam yepipette kwaye inokubangela ukuba incam ivuze okanye ibekwe kakubi, okanye ibangele ukuba incam yepipetti iwe ngokupheleleyo. .Ukukhetha i-micropipette ephezulu eyenziwe ngeengcebiso ezithile iqinisekisa uxhulumaniso olukhuselekileyo, ukubonelela ngezinga eliphezulu lokuthembeka kunye neziphumo ezingcono.Ukongezelela, into elula njenge-coding color-coding pipettes kunye neengcebiso zinokunceda abasebenzisi baqinisekise ukuba iingcebiso ezichanekileyo zikhethiweyo. iipayipi zabo.
Kwindawo ephezulu, kubalulekile ukuba usebenze ngokusemandleni ngelixa ugcina ukuthembeka kunye nokuhambelana kwenkqubo yokubhobhoza.Kukho iindlela ezininzi zokuphucula ukusebenza kakuhle kwepayipi, kubandakanywa nokusetyenziswa kwe-multichannel kunye / okanye i-pipettes ye-elektroniki.Ezi zixhobo eziguquguqukayo. zihlala zibonelela ngeendlela ezininzi ezahlukeneyo zepayipi-ezifana ne-reverse pipetting, i-variable dispensing, i-dilutions ye-serial ecwangcisiweyo, kunye nokunye-ukwenza lula inkqubo.Ngokomzekelo, iinkqubo ezifana nokuphindaphinda ngokuphindaphindiweyo zilungele ukukhupha i-aliquots ezininzi zomthamo ofanayo ngaphandle kokuzalisa kwakhona incam. Ukusebenzisa i-pipette ye-channel-channel ukudlulisa iisampulu phakathi kweefomathi ezahlukeneyo ze-labware inokukhawuleza ibe yinto edinayo kakhulu kwaye i-prone-prone.Iipayipi ze-Multichannel zivumela ukuhanjiswa kweesampuli ezininzi ngexesha elinye ngokuqhwanyaza kweliso.Akukuphela nje oku kwandisa ukusebenza kakuhle, kukwanceda ukukhusela ipayipi. iimpazamo kunye nokulimala okuphindaphindayo (RSI) .Ezinye iipipettes zide zikwazi ukuhluka kwisithuba se-tip ngexesha lokubhobhoza, ukuvumela ukuhanjiswa okufanayo kweesampuli ezininzi phakathi kobukhulu obuhlukeneyo be-labware kunye neefomathi, ukugcina iiyure zexesha (Umfanekiso 3).
Iingcali zelabhoratri zihlala zichitha iiyure ngosuku nge-pipetting.Oku kunokubangela ukuphazamiseka kwaye, kwiimeko ezinzima kakhulu, nokuba yisandla okanye ukulimala kwengalo.Ingcebiso efanelekileyo yokuphepha le mingcipheko enokubakho kukunciphisa ixesha lokubamba i-pipette kwixesha elifutshane. .Ukongezelela kule nto, abasebenzisi kufuneka bakhethe i-micropipette ekhaphukhaphu kunye nokulungelelaniswa kakuhle kunye nobunzima phakathi kwiziko lokuzinza okungcono. umthamo ukhululekile kwaye ngokukhawuleza kunokwenzeka ukuba ugweme ukunyakaza okungadingekile.Kwakhona, iingcebiso zibalulekile, njengoko ukulayishwa kweengcebiso kunye ne-ejection kudla ngokufuna amandla angaphezulu kunombhobho kwaye kukho umngcipheko wokulimala, ngokukodwa kwiindawo eziphezulu.Iingcebiso zePipette kufuneka zifake endaweni ngamandla amancinci, nikezela ngoqhagamshelwano olukhuselekileyo, kwaye kube lula ngokulinganayo ukukhupha.
Xa ukhetha i-micropipette efanelekileyo kwisicelo sakho, kubalulekile ukujonga zonke iinkalo zokuhamba komsebenzi wakho.Ngokuqwalasela i-pipette, iimpawu zayo, uhlobo kunye nomthamo wolwelo olufakwe kwipayipi, kunye neengcebiso ezisetyenzisiweyo, izazinzulu zinokuqinisekisa ukuba zichanekile, zichanekile kwaye zithembekile. iziphumo ngelixa ugcina imveliso kunye nokunciphisa umngcipheko wokulimala.
Kolu shicilelo, ukubuyiswa kwabahlalutyi abasisiseko kuhlolwa yi-HPLC-MS kusetyenziswa imowudi edibeneyo yokutshintshiselana nge-cation exchange SPE microplates. Izibonelelo ze-SEC-MALLS kwizicelo ze-biopharmaceutical...
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Ixesha lokuposa: Jun-10-2022