Iipleyiti zequla elinzulu luhlobo lwezixhobo zelabhoratri ezisetyenziswa kwinkcubeko yeeseli, uhlalutyo lwe-biochemical, kunye nezinye izicelo zesayensi. Ziyilelwe ukubamba iisampuli ezininzi kumaqula ahlukeneyo, ukuvumela abaphandi ukuba benze imifuniselo kumlinganiselo omkhulu kunezitya zepetri zemveli okanye iityhubhu zovavanyo.
Iipleyiti zequla ezinzulu ziza kwiintlobo ezahlukeneyo zobukhulu kunye neemilo, ukusuka kwi-6 ukuya kwi-96 yequla. Ezona zixhaphakileyo ziipleyiti ezinamaqula angama-96, ezinoxande ngokwemilo kwaye zihlalisa amaqula eesampuli zomntu ngamnye kwimiqolo eyi-8 ngeekholamu ezili-12. Umthamo womthamo wequla ngalinye uyahluka ngokobukhulu bawo, kodwa ngokuqhelekileyo uphakathi kwe-0.1 mL - 2 mL ngequla ngalinye. Iipleyiti zequla elinzulu nazo ziza kunye neziciko ezinceda ukukhusela iisampulu kungcoliseko ngexesha lokugcinwa okanye ukuthutha kwaye zibonelele ngesitywina esingangeni moya xa zibekwe kwi-incubator okanye ishaker ngexesha lovavanyo.
Iipleyiti zamaqula anzulu zinosetyenziso oluninzi kushishino lwesayensi yobomi; zisetyenziswa ngokuqhelekileyo kwinkcubeko yeeseli, ezifana nezifundo zokukhula kwebhaktheriya, iimvavanyo ze-cloning, iindlela ze-DNA extraction / amplification ezifana ne-PCR (i-polymerase chain reaction) kunye ne-ELISA (i-enzyme-linked immunosorbent assay). Ukongeza, iipleyiti zequla ezinzulu zinokusetyenziselwa izifundo ze-enzyme kinetic, iimvavanyo zokujonga i-antibody, kunye neeprojekthi zophando lokufunyanwa kweziyobisi, phakathi kwabanye.
Iiplati ze-96-well deep-well-well zinika inzuzo ebalulekileyo ngaphezu kwezinye iifomathi njengoko zinyusa indawo yommandla kumlinganiselo wevolumu - xa kuthelekiswa neefomathi ezincinci ezifana ne-24- okanye i-48-well-plates, oku kuvumela ukuba iiseli ezininzi okanye iimolekyuli ziqhutywe ngexesha elinye. ngelixa uGcina amanqanaba esisombululo esaneleyo ngokwahlukeneyo kwiidiski. Ukongezelela, ezi ntlobo zamacwecwe zenza ukuba izazinzulu zizenzele ngokukhawuleza iinkqubo zisebenzisa iinkqubo zerobhothi, ukwandisa kakhulu amandla okuphumelela ngaphandle kokunciphisa amanqanaba okuchaneka; into engenzekiyo kusetyenziswa iindlela zesintu ezifana nemibhobho yezandla.
Isishwankathelo, kucacile ukuba kutheni iipleyiti ze-96-deep-qula zisetyenziswa ngokubanzi kwiindawo ezininzi ezahlukeneyo zophando lwezenzululwazi; ngenxa yobukhulu befomathi yabo, bavumela abaphandi ukuba babe bhetyebhetye ngakumbi ekwenzeni imifuniselo ngelixa bebonelela ngexesha elisebenzayo lokucubungula, beyenza ilungele iilabhoratri zanamhlanje emhlabeni jikelele!
Ixesha lokuposa: Feb-23-2023