Ungazikhetha njani iipleyiti zePCR ezifanelekileyo kunye neityhubhu kwisicelo sakho?

I-Polymerase chain reaction (PCR) bubuchule obusetyenziswa ngokubanzi kwibhayoloji yemolekyuli ukukhulisa amaqhekeza eDNA. I-PCR ibandakanya amanyathelo aliqela, kubandakanywa i-denaturation, i-annealing, kunye nokwandiswa. Ukuphumelela kolu buchule kuxhomekeke kakhulu kumgangatho weepleyiti zePCR kunye neetyhubhu ezisetyenzisiweyo. Zininzi izinto ekufuneka uziqwalasele xa ukhetha iipleyiti zePCR ezifanelekileyo kunye neityhubhu zesicelo sakho.Nantsi imiba ephambili ekufuneka uyiqwalasele:

1. UmthamoIipleyiti zePCRkwaye iityhubhu ziza ngobukhulu obahlukeneyo kunye nobuchule. Ukukhethwa kobukhulu kunye nomthamo kuxhomekeke ubukhulu becala kubungakanani be-DNA ekufuneka yandiswe ngendlela enye. Ngokomzekelo, ukuba ufuna ukukhulisa inani elincinci le-DNA, unokukhetha ityhubhu encinci. Ukuba inani elikhulu le-DNA lifuna ukunyuswa, ipleyiti enomthamo omkhulu inokukhethwa.

2. Iipleyiti ze-PCR eziphathekayo kunye neetyhubhu zingenziwa ngezinto ezahlukeneyo ezifana ne-polypropylene, i-polycarbonate okanye i-acrylic. I-Polypropylene yeyona nto isetyenziswa ngokuqhelekileyo ngenxa yeekhemikhali zayo kunye nokumelana nobushushu. Ikwabiza kancinci xa kuthelekiswa nezinye izinto. Iipolycarbonates kunye ne-acrylics zibiza kakhulu, kodwa zicace ngcono kwaye zifanelekile kwi-PCR yexesha langempela.

3. I-PCR ye-Thermal conductivity ibandakanya imijikelezo emininzi ye-thermal, efuna ukufudumeza ngokukhawuleza kunye nokupholisa umxube wokuphendula. Ngoko ke, iipleyiti ze-PCR kunye neetyhubhu kufuneka zibe ne-conductivity efanelekileyo ye-thermal ukuqinisekisa ukufudumeza okufanayo kunye nokupholisa umxube wokuphendula. Amacwecwe aneendonga ezincinci kunye neendawo ezisicaba zilungele ukunyusa ukuhanjiswa kobushushu.

4. Ukuhambelana kweeplate zePCR kunye neetyhubhu kufuneka zihambelane ne-thermal cycler oyisebenzisayo. Iipleyiti kunye neetyhubhu kufuneka zikwazi ukumelana nobushushu obuphezulu obufunekayo ekukhuliseni amaqhekeza eDNA. Soloko udibana nomenzi webhayisekile eshushu malunga neepleyiti ezicetyiswayo kunye neetyhubhu.

5. Ukutywina Itywina eliqinileyo libalulekile ukuthintela ukungcoliseka komxube wokusabela. Iipleyiti ze-PCR kunye neetyhubhu zinokutywinwa kusetyenziswa iindlela ezahlukeneyo ezifana nokutywinwa kobushushu, iifilimu ezincamathelayo okanye izivalo. Ukutywinwa kobushushu yeyona ndlela ikhuselekileyo kwaye ibonelela ngomqobo onamandla ngokuchasene nokosuleleka.

6. Iipleyiti ze-PCR zokuvala inzala kunye neetyhubhu mazingabi nazingcolisi ezinokuthi ziphazamise ukusabela. Ke ngoko, kufuneka zicocwe phambi kokusetyenziswa. Kubalulekile ukukhetha iipleyiti kunye neetyhubhu ekulula ukuzivala kwaye zixhathise kwiindlela zokuvala iikhemikhali kunye nobushushu.

Isishwankathelo, ukukhetha ipleyiti yePCR efanelekileyo kunye neetyhubhu zibalulekile kulwandiso lweDNA oluyimpumelelo. Ukhetho luxhomekeke kakhulu kuhlobo lwesicelo, inani le-DNA ekhulisiwe, kunye nokuhambelana neebhayisikili ze-thermal.

I-Suzhou Ace Biomedical Technology Co., Ltd. inikeza uluhlu oluphezulu lweepleyiti ze-PCR kunye neetyhubhu ngobukhulu obuhlukeneyo, amandla kunye nezixhobo ukuhlangabezana neemfuno zomphandi ngamnye.