Iimpazamo ezi-5 eziQhelekileyo onokuziphepha xa usebenzisa iingcebiso zePipette kwiLebhu
1. Ukukhetha OkungalungangaIngcebiso kaPipette
Ukukhetha incam yepipette echanekileyo ibalulekile ekuchanekeni nasekuchanekeni kovavanyo lwakho. Enye impazamo eqhelekileyo kukusebenzisa uhlobo olungalunganga okanye ubungakanani bencam yepipette. Ingcebiso nganye yenzelwe izicelo ezithile, kwaye ukusebenzisa ingcebiso engalunganga kunokukhokelela kwiziphumo ezingahambelaniyo kunye ne-reagents elahlekileyo.
Ukuze ugweme le mpazamo, hlala ubhekisa kwizikhokelo zomenzi okanye uqhagamshelane neengcali kwintsimi. Cinga izinto ezinjengokuhambelana kwengcebiso kunye nepipette, umthamo wesampulu efunekayo, kunye nohlobo lovavanyo oluqhubayo. Ngokukhetha i-tip ye-pipette efanelekileyo, unokuqinisekisa ukusebenza kakuhle kunye neziphumo ezithembekileyo.
2. Ukuqhotyoshelwa kweNgcebiso engafanelekanga
Ukuqhotyoshelwa okungafanelekanga kwencam yepipette enye impazamo enokuthi idibanise ukuchaneka nokuchaneka. Ukuba i-tip ayiqhotyoshelwanga ngokukhuselekileyo, inokukhulula okanye idibanise ngexesha lenkqubo yepayipi, ekhokelela ekulahlekeni kwesampuli kunye nokungcoliswa.
Ukuze ugweme oku, landela imiyalelo yomenzi wokudibanisa i-pipetti ngokuchanekileyo. Qinisekisa ukuba incam ihambelana ngokuqinileyo kwaye ikhuselekile kwi-nozzle ye-pipette. Ukongezelela, jonga rhoqo i-tip nayiphi na impawu yokuguga okanye umonakalo, kwaye uyibuyisele xa kuyimfuneko. Uncamathiselo oluchanekileyo lwengcebiso luyimfuneko kwiziphumo ezithembekileyo nezinokuphinda zenziwe kwakhona.
3. Ukubetha umbhobho ongaphezulu okanye ukubetha umbhobho ongaphantsi
Imibhobho echanekileyo ibandakanya ukulinganisa ngononophelo kunye nokudlulisa umthamo ofunekayo wolwelo. Iimpazamo ezimbini eziqhelekileyo ezinokuthi zenzeke ngexesha lale nkqubo kukugqithisa imibhobho kunye nokubetha ngaphantsi. Ukugqithisa imibhobho kubhekiselele ekugqithiseni umthamo ofunekayo, ngelixa ukuhambisa umbhobho ongaphantsi kuthetha ukubhobhoza ngaphantsi kwesixa esifunekayo.
Zombini iimpazamo zingakhokelela kwiimpazamo ezibalulekileyo kwiziphumo zakho zovavanyo. Ukugqithisa imibhobho kunokubangela ukuhlanjululwa kweesampulu okanye ii-reagents, ngelixa ukubetha kombhobho ongaphantsi kunokukhokelela ekugxininiseni okungonelanga okanye imixube yokusabela.
Ukuze ugweme ukugqithisa imibhobho okanye ukubethelwa kombhobho ongaphantsi, qiniseka ukuba usebenzisa ubuchule obufanelekileyo bokufaka imibhobho. Ziqhelanise nokulinganisa kwe-pipette kunye nemida yokubhobhoza. Beka ivolumu ngokufanelekileyo, uqinisekise ipayipi echanekileyo yomthamo ofunekayo. Lungiselela rhoqo i-pipettes yakho ukuze ugcine ukuchaneka nokuchaneka.
4. Ukuchukumisa umgqomo weSampuli
Usulelo luyinkxalabo enkulu kuyo nayiphi na indawo yaselabhoratri. Enye impazamo eqhelekileyo eyenziwa ngabaphandi kukuchukumisa ngempazamo isikhongozeli sesampulu ngencam yepipette. Oku kunokwazisa amasuntswana angaphandle okanye izinto kwisampulu, ekhokelela kwiziphumo ezingachanekanga.
Ukuthintela le mpazamo, khumbula ukunyakaza kwakho kwaye ugcine isandla esizinzileyo ngelixa ubeka umbhobho. Gwema ukubeka uxinzelelo olugqithiseleyo kwi-pipette okanye ukusebenzisa amandla angeyomfuneko xa uhambisa okanye unqwenela. Ukongeza, beka incam kufutshane nomphezulu wolwelo ngaphandle kokuchukumisa iindonga zesikhongozeli. Ngokuziqhelanisa nobuchwephesha obulungileyo bemibhobho, unokunciphisa umngcipheko wongcoliseko lwesampulu.
5. IiNdlela zokuNika ezingalunganga
Impazamo yokugqibela ekufuneka uyiphetshwe ziindlela ezingachanekanga zokusasaza. Unikezelo olungafanelekanga lunokubangela ukusasazwa okugwenxa okanye okungalinganiyo kolwelo, okuchaphazela ukunyaniseka kweziphumo zakho zovavanyo. Iimpazamo eziqhelekileyo ziquka ukukhupha okukhawulezayo okanye okungalawulwayo, ukuthontsiza, okanye ukushiya ngempazamo imiqulu eshiyekileyo kwincam.
Ukuqinisekisa ukuchithwa okuchanekileyo kunye nokuhambelanayo, qaphela isantya kunye ne-angle ye-pipette ngexesha lenkqubo. Gcina isantya esilawulwayo nesizinzileyo, ukuvumela ulwelo ukuba luhambe kakuhle. Emva kokukhupha, linda ixesha elifutshane ukuvumela nayiphi na i-liquid eseleyo ukuba ikhuphe ngokupheleleyo ngaphambi kokususa i-pipette kwisitya.
ukuphepha iimpazamo eziqhelekileyo xa usebenzisa iingcebiso ze-pipette kwilebhu kubalulekile ekufumaneni iziphumo ezinokwethenjelwa kunye nokuveliswa kwakhona. Ngokukhetha ithiphu yepipette echanekileyo, ukuyincamathisela ngokufanelekileyo, ukuziqhelanisa neendlela ezichanekileyo zokubeka umbhobho, ukuthintela ukungcoliseka kwesampulu, kunye nokusebenzisa iindlela ezichanekileyo zokuhambisa, unokuphucula ukuchaneka kunye nokuchaneka kovavanyo lwakho.
Ixesha lokuposa: Mar-06-2024