Hobaneng ha Lisebelisoa tsa Lab e Hlokahala Hore e be DNase le RNase Free?

Lefapheng la baeloji ea limolek'hule, ho nepahala le ho tšepahala ke tsa bohlokoa ka ho fetisisa. Tšilafalo leha e le efe e ka sebelisoang ka laboratoring e ka lebisa liphellong tse fosahetseng, tse ka bang le liphello tse matla bakeng sa lipatlisiso tsa saense le tlhahlobo ea mafu. Mohloli o mong o tloaelehileng oa tšilafalo ke boteng ba li-enzyme tsa DNase le RNase. Li-enzyme tsena li nyenyefatsa DNA le RNA, ka ho latellana, 'me li ka fumanoa matrices a fapaneng a likokoana-hloko. Ho fokotsa kotsi ea tšoaetso le ho netefatsa liphetho tse nepahetseng, lisebelisoa tsa laboratori, joalo kamalebela a pipette, lipoleiti tse tebileng tsa seliba, Lipoleiti tsa PCR, le li-tubes, e tlameha ho ba DNase le RNase mahala.

Li-enzyme tsa DNase le RNase li fumaneha hohle 'me li ka fumanoa mehloling e fapaneng ea likokoana-hloko, ho kenyeletsa 'mele oa motho, limela le likokoana-hloko. Ba bapala karolo ea bohlokoa lits'ebetsong tsa cellular joalo ka karohano ea DNA, tokiso ea DNA, le ho senyeha ha RNA. Leha ho le joalo, ho ba teng ha tsona sebakeng sa laboratoring ho ka ba kotsi litekong tse amanang le tlhahlobo ea DNA le RNA.

Malebela a Pipette ke e 'ngoe ea lisebelisoa tsa laboratori tse sebelisoang hangata. Li sebelisetsoa ho sebetsana le mokelikeli o nepahetseng le o nepahetseng, e leng ho etsang hore e be tsa bohlokoa bakeng sa lits'ebetso tse fapaneng tse kang ho lokisa sampole, tatellano ea DNA, le PCR. Haeba malebela a pipette e se DNase le RNase mahala, tšilafalo e ka etsahala nakong ea pipetting, e lebisang ho senyeha ha lisampole tsa DNA kapa RNA. Sena se ka fella ka liphello tse fosahetseng kapa tse sa tsitsang, tse senyang botšepehi ba teko eohle.

Lipoleiti tse tebileng tsa seliba ke laboratori e 'ngoe ea bohlokoa e ka jeoang, haholo lits'ebetsong tse sebetsang hantle haholo. Li sebelisoa bakeng sa polokelo ea mehlala, li-dilutions tsa serial, le setso sa lisele. Haeba lipoleiti tsena li se na DNase le RNase mahala, lisampole leha e le life tsa DNA kapa RNA tse bolokiloeng ho tsona li ka silafala, tse lebisang ho senyeha ha nucleic acid. Sena se ka senya ho nepahala ha lits'ebetso tse tlase tse kang PCR, qPCR, kapa tatellano ea moloko o latelang.

Ka mokhoa o ts'oanang, lipoleiti tsa PCR le li-tubes ke likarolo tsa mantlha lits'ebetsong tsa polymerase chain reaction (PCR). PCR ke mokhoa o sebelisoang haholo ho holisa tatellano ea DNA. Haeba lipoleiti tsa PCR le li-tubes li silafalitsoe ke DNase kapa RNase, mokhoa oa ho hōlisa o ka senyeha, o lebisa ho liphello tse sa nepahalang le litlhaloso tse fosahetseng. Lisebelisoa tsa PCR tsa DNase le RNase-free PCR li thibela ho senyeha ha DNA kapa RNA e lebisitsoeng nakong ea ts'ebetso ea ho hōlisa, ho netefatsa liphello tse ka tšeptjoang le tse ka hlahisoang hape.

Ho rarolla bothata ba ts'oaetso, lisebelisoa tsa laboratori li hloka ho etsoa ka lits'ebetso tse laoloang haholo le lisebelisoa tse netefalitsoeng hore ha li na DNase le RNase mahala. Likhamphani tse kang Suzhou Ace Biomedical Technology Co., Ltd., li sebetsa ka ho khetheha tlhahisong ea lisebelisoa tsa laboratori tse fihlelang litlhoko tsena tse thata. Joaloka moetsi ea ka sehloohong tšimong, Suzhou Ace Biomedical Technology Co., Ltd. e etelletsa pele boleng le ho tšepahala.

Suzhou Ace Biomedical Technology Co., Ltd. e utloisisa boemo bo mahlonoko ba tšilafalo ea DNase le RNase linthong tse ka sebelisoang laboratoring. Malebela a bona a pipette, lipoleiti tse tebileng tsa seliba, lipoleiti tsa PCR, le li-tubes kaofela li entsoe ka lisebelisoa tsa boleng bo holimo tse nkang mehato e matla ea taolo ea boleng ho netefatsa hore ha li na DNase le RNase mahala.

Khampani e sebelisa mekhoa e tsoetseng pele ea tlhahiso 'me e khomarela litekanyetso tse tiileng tsa boleng ho felisa kotsi ea tšoaetso, kahoo e fana ka liphetho tse nepahetseng le tse ka tšeptjoang bakeng sa bafuputsi le lingaka ka ho tšoanang. Ba utloisisa hore ho sekisetsa hofe kapa hofe boleng ba lisebelisoa tsa laboratori ho ka ba le litlamorao tse kholo, eseng feela liphuputsong empa le lits'ebetsong tsa bongaka moo tlhahlobo e nepahetseng e leng bohlokoa.

Qetellong, lisebelisoa tsa laboratori tse kang malebela a pipette, lipoleiti tsa liliba tse tebileng, lipoleiti tsa PCR, le li-tubes li tlameha ho ba DNase le RNase mahala ho netefatsa ho nepahala le ho tšepahala ha liteko tsa baeloji ea limolek'hule. Ho silafatsoa ka li-enzyme tsena ho ka lebisa ho senyeha ha lisampole tsa DNA le RNA, ho senya bonnete ba liphetho tse fumanoeng. Lik'hamphani li rataSuzhou Ace Biomedical Technology Co., Ltd. utloisisa bohlokoa ba ho etsa thepa e sebelisoang ho fihlela litlhoko tsena tse thata, ho nolofalletsa bo-ramahlale le lingaka ho etsa mosebetsi oa bona ka kholiseho le ka nepo.