Ke lipoleiti life tseo ke lokelang ho li khetha bakeng sa The Extraction of Nucleic Acid?

Khetho ea lipoleiti bakeng sa ho ntša nucleic acid e itšetlehile ka mokhoa o khethehileng oa ho ntša o sebelisoang. Mekhoa e fapaneng ea ho ntšoa e hloka mefuta e fapaneng ea lipoleiti ho fihlela litholoana tse ntle. Mona ke mefuta e 'maloa ea lipoleiti tse sebelisoang hangata bakeng sa ho ntšoa ha nucleic acid:

1. Lipoleiti tsa PCR tse 96 hantle: Lipoleiti tsena li atisa ho sebelisoa bakeng sa mekhoa e phahameng ea ho ntša nucleic acid. Li tsamaisana le litsamaiso tse sebetsanang le mokelikeli o ikemetseng 'me li ka tšoara sampole e nyane.
2. Lipoleiti tse tebileng tsa seliba: Lipoleiti tsena li na le bophahamo bo boholo ho feta lipoleiti tsa PCR 'me li sebelisetsoa mekhoa ea matsoho kapa ea automated nucleic acid e hlokang lisebelisoa tse kholoanyane tsa sampuli.
3. Spin litšiea: Litšiea tsena li sebelisetsoa mekhoa ea matsoho ea ho ntša nucleic acid e hlokang ho hloekisoa le ho tsepamisa mohopolo oa nucleic acid. Litšiea li na le lesela le thehiloeng ho silica le kopanyang nucleic acid le ho li arola ho tse ling tse silafatsang.
4. Lifaha tsa Magnetic: Hangata lifaha tsa Magnetic li sebelisoa bakeng sa mekhoa e ikemetseng ea ho ntša nucleic acid. Lifaha li koahetsoe ka thepa e tlamang ho nucleic acids 'me e ka arohanngoa habonolo le litšila tse ling ho sebelisa makenete.

Ho bohlokoa ho shebana le protocol kapa kit e khethehileng e sebelisoang bakeng sa ho ntša nucleic acid ho fumana mofuta o nepahetseng oa poleiti bakeng sa mokhoa ona.

Lisebelisoa tsa rona tsa Extraction of Nucleic Acid li etselitsoe ho fana ka ts'ebetso e tšepahalang le e sebetsang ea DNA le RNA ho tsoa mefuteng e fapaneng ea sampole. Lisebelisoa tsa rona li tsamaisana le mefuta e mengata ea mekhoa le liforomo, ho kenyelletsa le mekhoa ea matsoho le ea boiketsetso.

Sehlahisoa sa rona sa mohala se kenyelletsaLipoleiti tsa PCR, lipoleiti tse tebileng tsa seliba, li-spin columns, le lifaha tsa makenete, kaofela li etselitsoe ho khotsofatsa litlhoko tsa liprothokholo tse fapaneng tsa ho ntša. Lipoleiti tsa rona tsa PCR le lipoleiti tse tebileng tsa seliba li entsoe ka thepa ea boleng bo holimo ho netefatsa hore li tsamaellana le litsamaiso tse sebetsanang le mokelikeli o ikemetseng le ho mamella liprothokholo tse thata tsa ho ntšoa. Litšiea tsa rona tse pota-potileng li na le lesela le thehiloeng ho silica le fanang ka tlamahano e babatsehang ea li-nucleic acid le ho tlosoa ka katleho ha litšila. Lifaha tsa rona tsa makenete li koahetsoe ka thepa ea thepa e fanang ka matla a holimo a tlamang le karohano e sebetsang ea li-nucleic acid ho tsoa ho likarolo tse ling tsa sampole.

Lisebelisoa tsa rona tsa Extraction of Nucleic Acid li lekoa haholo bakeng sa ts'ebetso le boleng ho netefatsa liphetho tse tsitsitseng. Re ikemiselitse ho fa bareki ba rona lisebelisoa tsa boleng bo holimo ho ts'ehetsa litlhoko tsa bona tsa nucleic acid.

Ka kopo ikopanye le rona bakeng sa tlhaiso-leseling e batsi mabapi le lisebelisoa tsa rona tsa Extraction of Nucleic Acid le hore na li ka u tsoela molemo joang lipatlisiso tsa hau kapa likopo tsa tlhahlobo ea mafu.