Linoko tsa vitro li bolela ts'ebetso ea ho hlahloba lefu kapa boemo ka ho kenyelletsa sampole ea bioloji ho tsoa ka ntle ho mmele. Ts'ebetso ena e sebetsa haholo mekhoa e fapaneng ea lisele tsa biology, ho kenyelletsa le nucleic acid e tlohang. Ntle le moo, mokelikeli o ts'oareha ke karolo ea bohlokoa ea li-khetho tsa vitros.
Boitšoaro ba PR kapa polymerase sa karabelo ke mokhoa o sebelisoang ho holisa likotoana tsa DNA. Ka ho sebelisa litlhare tse ikhethileng, Pcc e lumella ho holisa tatellano ea tatellano ea li-DNA, ebe e ka hlahlojoa matšoao a lefu kapa tšoaetso. PCR e sebelisoa hangata ho bona vaerase, bacterial, tšoaetso ea fungal le ea parasitic, hammoho le mafu a liphatsa tsa lefutso le mofets'e.
Nonoloction ea Nucleic acid ke mokhoa o sebelisitsoeng ho khetholla le ho hloekisa DNA kapa Rna ho tsoa sampole ea biology. Li-acid tsa nyutlelie tse nkiloeng li fumaneha bakeng sa tlhahlobo e 'ngoe, ho kenyelletsa le PCR. Nucleic acid ho tsoaloa bakeng sa tlhahlobo e nepahetseng ea tlhahlobo le tatellano ea kalafo ea mafu le maemo a fapaneng.
Ho sebetsana le metsi ke ts'ebetso e kenyelletsang phetiso e nepahetseng, ho phatlalatsa le ho kopanya metsi a mangata ka har'a laboratori. Tsamaiso ea metsi e tsamaisang metsi e ntse e ratoa ke ho feta lilemong tsa morao tjena ha li ntse li nolofatsa tlhahiso le ho nepahala tse ngata tse thusang le Nucleic acid.
Linono tsa vitroro li itšetlehile haholo ka mekhoa ena ea biogy e ntle hobane e lumella ho fumanoa le tlhahlobo ea matšoao a amanang le lefu la lefutso le lisele. Mohlala, PCC e ka sebelisoa ho holisa likarolo tsa matsoele tse amanang le kankere ea matsoele, ha ho ntšoa ka lebaka la kankere ea matsoele, ha ho ne ho tla sebelisoa kankere ea nyutsi ho ka sebelisoa ho khetholla DNA.
Ntle le mekhoa ena, mekhoa e meng e fapaneng ea mefuta le lisebelisoa li sebelisoa ho lingaka tsa vitroro. Mohlala, lisebelisoa tsa Microfluidic li sebelisoa le ho feta ho litlokotsebe le lits'ebetsong tsa tlhokomelo ea tlhokomelo. Lisebelisoa tsena li etselitsoe ho sebetsana le likotsi tse nyane, li li etsa hore li lokele PCR le likopo tse ling tsa biology tsa biology.
Ka mokhoa o ts'oanang, mahlale a latelang a mofuta o latelang - Li-NGS) Mahlale a NGS a ntse a bapala karolo ea bohlokoa ea liketsahalo tsa vitroro. NGS e nolofalletsa ho leka-lekana ha liquene tsa limilione tsa DNA, ho lemoha liphetoho tse amanang le lefu. NGS e na le monyetla oa ho fumana tlhahlobo le kalafo ea mafu a liphatsa tsa lefutso le mofetše.
Ka kakaretso, khetho ea vitro ke karolo ea bohlokoa ea meriana ea sejoale-joale 'me e itšetlehe haholo ka mekhoa ea bigy ea biology e joalo ka PCR, le ho sebetsana le nyutlelie. Mahlale ana, hammoho le lisebelisoa tsa merafong le nyona, li fetoha tsela eo re bonang ka eona le ho phekola maloetse le ho phekola maloetse. Ha thesehlahlalisi e ntse e tsoela pele, khetho ea vitraro e kanna ea sebetsa le ho feta, qetellong ho ntlafatsa liteko tsa bophelo le boleng ba mokuli.
At Suzhou Ace Biomedical,Re ikemiselitse ho u fa moputso oa boleng bo holimo haholo bakeng sa litlhoko tsohle tsa mahlale. Malebela a rona a maqhubu a pompotte, li-tubet tsa pcc, li-tubers tsa pcr, le filimi e tsitsitseng li etselitsoe ka nepo ebile li entsoe ka mokhoa o hlakileng le ho nepahala ho liteko tsohle tsa hau. Malebela a rona a etellang pele a lumellana le mabitso ohle a maholo a liphapang 'me u tle ka mefuta e mengata ho lekana litlhoko tsa hau tse khethehileng. Lijana tsa rona tsa pcc le li-tubes li entsoe ka lisebelisoa tsa boleng bo holimo ebile li etselitsoe ho mamella potoloho e mengata ea tlhaho ha u ntse u boloka botšepehi ba mohlala. Filimi ea rona ea tiiso e fana ka tiiso e tiileng ho thibela mouoane le litšila tse tsoang linthong tse kantle. Re utloa bohlokoa ba lisebelisoa tse tšepahalang le tse sebetsang, ke ka lebaka leo re loanelang ho u fa lihlahisoa le litšebeletso tse ntlehali. Sehlopha sa rona sa litsebi se lula se fumaneha ho u thusa ka lipotso kapa lintho tse u tšoenyang tseo u ka bang le tsona.
Nako ea poso: Mots'eanong-10-2023