in vitro diagnosis ke eng?

In vitro diagnostics e bolela mokhoa oa ho fumana lefu kapa boemo ka ho arola lisampole tsa tlhaho tse tsoang kantle ho 'mele. Ts'ebetso ena e itšetlehile haholo ka mekhoa e fapaneng ea baeloji ea limolek'hule, ho kenyeletsoa PCR le ho ntšoa ha nucleic acid. Ho feta moo, ts'ebetso ea mokelikeli ke karolo ea bohlokoa ea tlhahlobo ea in vitro.

PCR kapa polymerase chain reaction ke mokhoa o sebelisoang ho holisa likarolo tse itseng tsa DNA. Ka ho sebelisa li-primers tse khethehileng, PCR e lumella ho holisoa ka mokhoa o ikhethileng oa tatellano ea DNA, e ka hlahlojoang bakeng sa matšoao a lefu kapa tšoaetso. PCR e atisa ho sebelisoa ho lemoha tšoaetso ea kokoana-hloko, baktheria, fungal le parasitic, hammoho le mafu a lefutso le kankere.

Nucleic acid extraction ke mokhoa o sebelisetsoang ho arola le ho hloekisa DNA kapa RNA ho tloha lisampoleng tsa likokoana-hloko. Li-nucleic acid tse nkiloeng joale lia fumaneha bakeng sa tlhahlobo e eketsehileng, ho kenyeletsoa PCR. Ho ntšoa ha Nucleic acid ho bohlokoa bakeng sa tlhahlobo e nepahetseng le moralo oa kalafo bakeng sa mafu le maemo a fapaneng.

Ho sebetsana le mokelikeli ke ts'ebetso e kenyeletsang phetiso e nepahetseng, ho fana le ho tsoakoa ha mekelikeli e nyane sebakeng sa laboratoring. Sistimi ea othomathike ea ho sebetsana le mokelikeli e se e tumme haholo lilemong tsa morao tjena kaha e thusa ho feta le ho nepahala ho hoholo ha liteko tse kang PCR le nucleic acid extract.

Litlhahlobo tsa in vitro li itšetleha haholo ka mekhoa ena ea baeloji ea limolek'hule hobane li lumella ho fumanoa le tlhahlobo ea matšoao a amanang le lefutso le limolek'hule tse amanang le mafu. Mohlala, PCR e ka sebelisoa ho holisa tatellano ea liphatsa tsa lefutso tse amanang le mofetše oa matsoele, athe nucleic acid extract e ka sebelisoa ho arola DNA e tsoang ho tumor ho lisampole tsa mali.

Ntle le mekhoa ena, ho sebelisoa mekhoa le lisebelisoa tse ling ho in vitro diagnostics. Ka mohlala, lisebelisoa tsa microfluidic li ntse li sebelisoa ka ho eketsehileng lits'ebetsong tse phahameng le tsa tlhokomelo ea ntlha. Lisebelisoa tsena li etselitsoe ho sebetsana ka nepo le ho laola likhahla tse nyane, ho li etsa tse loketseng PCR le lits'ebetso tse ling tsa baeloji ea limolek'hule.

Ka mokhoa o ts'oanang, mahlale a morao-rao a latellanang (NGS) a ntse a bapala karolo ea bohlokoa le ho feta tlhahlobong ea in vitro. NGS e thusa tatellano e tšoanang ea likhechana tsa DNA tse limilione, e nolofalletsang ho lemoha ka potlako le ka nepo liphetoho tsa lefutso tse amanang le lefu. NGS e na le monyetla oa ho fetola tlhahlobo le kalafo ea mafu a lefutso le mofetše.

Ka kakaretso, tlhahlobo ea in vitro ke karolo ea bohlokoa ea bongaka ba sejoale-joale ebile e itšetlehile haholo ka mekhoa ea baeloji ea limolek'hule joalo ka PCR, nucleic acid extraction, le ho sebetsana le metsi. Dithekenoloji tsena, mmoho le dithekenoloji tse kang disebediswa tsa microfluidic le NGS, di fetola tsela eo re hlahlobang le ho alafa mafu ka yona. Ha thekenoloji e ntse e tsoela pele, tlhahlobo ea in vitro e kanna ea nepahala le ho sebetsa, qetellong ea ntlafatsa sephetho sa mokuli le boleng ba bophelo.

At Suzhou Ace Biomedical,re ikemiselitse ho u fa lisebelisoa tsa laborateng tsa boleng bo holimo ka ho fetisisa bakeng sa litlhoko tsohle tsa hau tsa mahlale. Malebela a rona a mangata a pipette, lipoleiti tsa PCR, li-tubes tsa PCR, le filimi e tiisang li entsoe ka makhethe ebile li entsoe ka boqhetseke ho netefatsa ho nepahala le ho nepahala litekong tsohle tsa hau. Litlhahiso tsa rona tsa pipette li lumellana le mefuta eohle e meholo ea li-pipette 'me li tla ka boholo bo fapaneng ho lumellana le litlhoko tsa hau tse khethehileng. Lipoleiti tsa rona tsa PCR le li-tubes li entsoe ka thepa ea boleng bo holimo 'me li etselitsoe ho mamella lipotoloho tse ngata tsa mocheso ha li ntse li boloka botšepehi ba sampole. Filimi ea rona ea ho tiisa e fana ka tiiso e tiileng ho thibela ho tsoa mouoane le ho silafala ho tsoa likarolong tse ka ntle. Re utloisisa bohlokoa ba lisebelisoa tsa lab tse tšepahalang le tse sebetsang hantle, ke ka lebaka leo re lekang ka matla ho u fa lihlahisoa le litšebeletso tse ntle ka ho fetesisa. Sehlopha sa rona sa litsebi se lula se le teng ho u thusa ka lipotso kapa lintho tse u tšoenyang.