Lipatlisisong tsa liphatsa tsa lefutso le tsa bongaka, polymerase chain reaction (PCR) ke mokhoa o sebelisoang hangata ho holisa disampole tsa DNA bakeng sa liteko tse fapaneng. Ts'ebetso ena e itšetlehile haholo ka lisebelisoa tsa PCR tse bohlokoa bakeng sa teko e atlehileng. Sehloohong sena, re tšohla lisebelisoa tsa bohlokoa bakeng sa teko e felletseng ea PCR: lipoleiti tsa PCR, li-tubes tsa PCR, li-membrane tsa ho tiisa, le malebela a pipette.
PCR poleiti:
Lipoleiti tsa PCR ke tse ling tsa lisebelisoa tsa bohlokoa ka ho fetisisa tekong efe kapa efe ea PCR. Li etselitsoe ho palama libaesekele tse potlakileng tsa mocheso 'me li fana ka phetisetso ea mocheso e ts'oanang ka har'a bore bakeng sa ho ts'oaroa habonolo. Lipoleiti li fumaneha ka mefuta e fapaneng ea litlhophiso ho kenyelletsa 96-well, 384-well, le 1536-well.
Lipoleiti tsa PCR li entsoe ka polasetiki, e leng se etsang hore li tšepahale le ho li tšoara habonolo. Ho feta moo, lipoleiti tse ling tsa PCR li koahetsoe ka ho khetheha ho thibela ho tlama ha limolek'hule tsa DNA le ho thibela tšoaetso. Tšebeliso ea lipoleiti tsa PCR e bohlokoa ho fokotsa mehato e boima ea mosebetsi e kileng ea etsoa ka microcentrifuges kapa mechine ea PCR.
PCR tube:
Li-tubes tsa PCR ke li-tubes tse nyane, hangata tse entsoeng ka polypropylene, tse sebelisetsoang ho tšoara motsoako oa karabelo ea PCR nakong ea ho holisa. Li na le mebala e sa tšoaneng, empa tse tloaelehileng ka ho fetisisa li hlakile ebile lia fetoha. Li-tubes tse hlakileng tsa PCR hangata li sebelisoa ha basebelisi ba batla ho bona DNA e matlafalitsoeng hobane e pepenene.
Li-tubes tsena li etselitsoe ho mamella mocheso o phahameng le likhatello tse fumanoang mecheng ea PCR, e leng se etsang hore e be tse loketseng liteko tsa PCR. Ntle le ho holisa, li-tubes tsa PCR li ka sebelisoa bakeng sa lits'ebetso tse ling joalo ka tatellano ea DNA le tlhoekiso le tlhahlobo ea likhechana.
Filimi ea ho tiisa:
Filimi ea Seal ke filimi ea polasetiki e khomarelang e khomaretsoeng ka holim'a poleiti ea PCR kapa tube ho thibela ho tsoa mouoane le ho silafala ha motsoako oa karabelo nakong ea PCR. Lifilimi tsa ho tiisa li bohlokoa haholo litekong tsa PCR, kaha motsoako oa karabelo o pepesitsoeng kapa tšilafalo efe kapa efe ea tikoloho ka poleiti e ka senya bonnete le katleho ea liteko.
E entsoe ka polyethylene kapa polypropylene, ho itšetlehile ka kopo, lifilimi tsena tsa polasetiki li na le mocheso o matla haholo 'me li khona ho iketsetsa likoloi. Lifilimi tse ling li sehiloe esale pele bakeng sa lipoleiti le li-tubes tse itseng tsa PCR, ha tse ling li tla ka meqolo 'me li ka sebelisoa ka mefuta e fapaneng ea lipoleiti tsa PCR kapa li-tubes.
Litlhahiso tsa Pipette:
Malebela a Pipette ke lisebelisoa tsa bohlokoa bakeng sa liteko tsa PCR, kaha li sebelisoa ho fetisa metsi a manyane, joalo ka lisampole kapa li-reagents. Hangata li entsoe ka polyethylene 'me li ka tšoara metsi ho tloha ho 0.1 µL ho isa ho 10mL. Malebela a Pipette a ka lahloa 'me a reretsoe tšebeliso e le 'ngoe feela.
Ho na le mefuta e 'meli ea malebela a pipette - e tlhotliloeng le e sa hloekisoang. Malebela a sefeng a loketse ho thibela tšilafalo efe kapa efe ea aerosol kapa marotholi ho etsahala, athe malebela a sa sefeng a sebelisoa bakeng sa liteko tsa PCR ho sebelisoa lihlapolli tsa inorganic kapa litharollo tsa caustic.
Ka bokhutšoanyane, lipoleiti tsa PCR, li-tubes tsa PCR, li-membrane tsa ho tiisa, le malebela a pipette ke tse ling tsa lisebelisoa tsa motheo tse hlokahalang bakeng sa teko e felletseng ea PCR. Ka ho etsa bonnete ba hore u na le lisebelisoa tsohle tse hlokahalang, u ka etsa liteko tsa PCR hantle ka nepo le ka nepo eo u e hlokang. Ka hona, kamehla etsa bonnete ba hore u na le lisebelisoa tsena tse lekaneng tse fumanehang habonolo bakeng sa teko efe kapa efe ea PCR.
At Suzhou Ace Biomedical, re ikemiselitse ho u fa lisebelisoa tsa laborateng tsa boleng bo holimo bakeng sa litlhoko tsohle tsa hau tsa mahlale. Mefuta ea rona eamalebela a pipette, Lipoleiti tsa PCR, Li-tubes tsa PCR, lefilimi ea ho tiisali entsoe ka makhethe ebile li entsoe ka bokhabane ho netefatsa ho nepahala le ho nepahala litekong tsohle tsa hau. Litlhahiso tsa rona tsa pipette li lumellana le mefuta eohle e meholo ea li-pipette 'me li tla ka boholo bo fapaneng ho lumellana le litlhoko tsa hau tse khethehileng. Lipoleiti tsa rona tsa PCR le li-tubes li entsoe ka thepa ea boleng bo holimo 'me li etselitsoe ho mamella lipotoloho tse ngata tsa mocheso ha li ntse li boloka botšepehi ba sampole. Filimi ea rona ea ho tiisa e fana ka tiiso e tiileng ho thibela ho tsoa mouoane le ho silafala ho tsoa likarolong tse ka ntle. Re utloisisa bohlokoa ba lisebelisoa tsa lab tse tšepahalang le tse sebetsang hantle, ke ka lebaka leo re lekang ka matla ho u fa lihlahisoa le litšebeletso tse ntle ka ho fetesisa. Sehlopha sa rona sa litsebi se lula se le teng ho u thusa ka lipotso kapa lintho tse u tšoenyang.
Nako ea poso: May-08-2023