Mokhoa oa ho sebelisa PCR plate ho etsa liteko?

Lipoleiti tsa PCR (polymerase chain reaction) li sebelisoa ho etsa liteko tsa PCR, tse sebelisoang haholo lipatlisisong tsa baeloji ea limolek'hule ho holisa tatelano ea DNA.

Mona ke mehato e akaretsang ea ho sebelisa aLetlapa la PCRbakeng sa teko e tloaelehileng:

1. Lokisetsa motsoako oa hau oa karabelo ea PCR: Lokisetsa motsoako oa hau oa karabelo ea PCR ho latela protocol ea teko ea hau, eo hangata e kenyelletsang template ea DNA, li-primer tsa PCR, dNTPs, Taq polymerase, buffer le litlatsetso tse ling.
2. Eketsa motsoako oa karabelo poleiti ea PCR: U sebelisa pipette e nang le mechine e mengata kapa pipette ea letsoho, eketsa motsoako oa karabelo lilibeng tsa platelete ea PCR. Etsa bonnete ba hore u qoba ho kenya li-bubble tsa moea motsoakong oa karabelo, kaha sena se ka ama sephetho sa liteko tsa hau.
3. Kenya template ea hau ho motsoako oa karabelo: Ho ipapisitse le teko ea hau, ho ka 'na ha hlokahala hore u kenye template ea hau ea DNA ho motsoako oa karabelo. Haeba u sebelisa pipette ea likanale tse ngata, etsa bonnete ba hore u fetola malebela lipakeng tsa lisampole ho qoba tšilafalo.
4. Tiisa poleiti: Hang ha u se u kentse motsoako oa reaction le template DNA poleiting ea PCR, tiisa poleiti ka tiiso e loketseng, joalo ka filimi ea PCR e tiisang kapa cap strip.
5. Beha poleiti ka har'a thermocycler: Qetellong, beha poleiti ea PCR e tiisitsoeng ka har'a thermocycler 'me u tsamaise lenaneo la hau la PCR, leo hangata le nang le letoto la lipotoloho tsa mocheso tse lumellang DNA ho holisoa.

Ka mor'a hore karabelo ea PCR e phethe, u ka hlahloba lihlahisoa u sebelisa mekhoa e sa tšoaneng, e kang gel electrophoresis kapa sequencing. Etsa bonnete ba hore u latela liprothokholo tse ikhethileng tsa liteko tsa hau ho fumana liphetho tse nepahetseng le tse tšepahalang.

Suzhou Ace Biomedicalke moetsi ea ka sehloohong oa boleng bo holimoLisebelisoa tsa PCR. Re inehetse ho fana ka lisebelisoa tse tšepahalang le tse sebetsang hantle bakeng sa liteko tsa PCR ea hau, ka mefuta e mengata ea lihlahisoa tse etselitsoeng ho fihlela litlhoko tsa bafuputsi mafapheng a fapaneng.

Lisebelisoa tsa rona tsa PCR li kenyelletsaLipoleiti tsa PCR, li-tubes tsa PCR, likhoele tsa PCR, le lifilimi tse tiisang. Lihlahisoa tsohle tsa rona li entsoe ka thepa ea boleng bo phahameng, ho netefatsa hore li ka mamella mathata a ts'ebetso ea PCR le ho hlahisa liphello tse tsitsitseng le tse nepahetseng.

Ho Suzhou Ace Biomedical, re utloisisa bohlokoa ba ho nepahala litekong tsa hau tsa PCR. Ke ka lebaka leo lisebelisoa tsa rona tsa PCR li hlahisoang ka maemo a holimo ka ho fetisisa a boleng 'me li etsoa liteko tse matla ho netefatsa hore li kopana kapa li feta tebello ea hau. Lihlahisoa tsa rona li boetse li etselitsoe hore li lumellane le mefuta e mengata ea li-thermocyclers, e leng se etsang hore e be khetho e fapaneng bakeng sa bafuputsi ba li-lab tse fapaneng.

Hore na o etsa lipatlisiso tsa mantlha, tlhahlobo ea bongaka, kapa lits'ebetso tse ling, Suzhou Ace Biomedical e na le lisebelisoa tsa PCR tseo u li hlokang ho fihlela lipheo tsa hau. Re ikemiselitse ho fana ka lihlahisoa tse ikhethang le tšebeletso ea bareki, 'me re motlotlo ho ba balekane ba tšepahalang ba bafuputsi lefatšeng ka bophara.

Ikopanye le rona kajeno ho ithuta haholoanyane ka lisebelisoa tsa rona tsa PCR le hore na re ka tšehetsa lipatlisiso tsa hau joang.