Mohlomong micropipatte ke sesebelisoa se sebelisoang haholo la laboratoring. Ba sebelisoa ke bo-ramahlale ba likarolo tse fapaneng ho kenyelletsa labar, matsaranka ba sepetlele hammoho le ho fetisetsa selikalikoe ka bongata
Ha e ntse e khoptjoa ebile e ferekanya ho bona li-bubble tse mpe tsa moea haeba li sa bonoe kapa li hlokomolohuoa ho ka ba le tšusumetso e kholo holima litholoana.
Litaba tse monate ke hore ho na le mehato e bonolo eo u ka e nkang ho thibela li-bubble tsa moea, 'me u ntlafatse khotsofalo ea sepakapakeng le ho hlaka ha litholoana.
Ka tlaase mona, re hlahloba sephetho sa ho fumana bubble ea moea ntlheng ea hau ea pipette le seo u lokelang ho se etsa ka mor'a moo.
Litholoana tsa li-bubble kaPipette Tim
Le ha o sebelisa se nepahetseng ka ho fetisisa, bokaholimo ba lipehelo, ba hlokometsoe hantle, li hlokomolohuoa ka liphoso tsa sephetho sa hau. Ha bubble kena hoKeletsoE ka ba le sephetho se hlokahalang.
● Ha mosebelisi a ntse a etsa khokahano ea moea ba tlameha ho qeta nako a bala metsi a otlolohileng ka nepo, a hana ntlha mme o qale ho tsoela pele hape.
● Likhohlopo tse sa bonahaleng tsa moea li ka fella ka phetisetso e tlase ea bophahamo, ka hona e fetola boemo ba karabelo ea karabelo e lebisang ho liteko tse atlehileng le liphetho tse sa tšepahaleng.
Liphetho tsena li ka ba le litlamorao tse 'maloa (1).
● O ile a fokotseha atefisa ea lab - liteko le assays li tla tlameha ho phetoa, tsa mosebetsi o boima le tsa nama, tse ka bang matla haholo.
● Liphetho tsa tlhahlobo tse belaetsang kapa tse fosahetseng - haeba ho na le liphetho tse fosahetseng tse ka ba le litlamorao tse mpe ho kenyelletsa ho kenyelletsa le litholoana tse fosahetseng tsa mokuli.
● Ho ipehalloa ke libuka tse ngotsoeng ka letsoho tse tsoang likoranteng - haeba lithaka li sitoa ho ngang lijo tsa hau ka lebaka la li-bubble tse bolaeang lintho tse etsang hore li-parate li ka tlosoa.
Mekhoa e metle ea ho thibela li-bubble tsa moea
Boholo ba linyetleng tsa moea li tsoa ka malebela a pipette li bakoa ke phoso e sebetsang. Mokhoa o futsanehileng ka lebaka la koetliso e sa lekaneng kapa ho tepella hangata ke bothata ba.
Phepelo ke ts'ebetso e bohlale e hlokang tlhokomelo ea 110%, e koetliselitsoeng le ho ikoetlisa ho fihlela litholoana tse sa fetoheng le tse nepahetseng.
Ha ho na le lintho tse 'maloa tseo u ka li etsang ho fokotsa liphoso tse akaretsang, ka tlase re hlahile litloaelo tse ntle tse ka sebelisoang ho qoba li-bubble tse ntle ho qoba litlolo tsa moea hoMalebela a Pipette.
Ntlafatsa mokhoa oa mosebelisi
Pipette butle butle
Haeba plunger e lokolloa kapele ha e sa phehelle, li-bubble tsa moea li ka hlahisoa ntlheng. Sena se ka thatafalloa haholo ha fetise lino tsa li-viscous. Tšusumetso e tšoanang e ka ba teng haeba plunger e lokolloa kapele ho feta ho hana.
Ho qoba li-bubble tsa moea ha li khetholla, hlokomela ho sebetsa Pispita ea lipeipi tsa libuka ka mokhoa o boreleli, o sebelisa matla a feto-fetohang.
Sebelisa botebo bo hlakileng bo hlakileng
Ho sitoa ho qobella ntlheng ea pipetutara e tebileng ka tlase ho Meniscus ea sebaka sa metsi ho ka baka ho kheloha moea le mokhoa oa bubble.
Leha ho le joalo, ho qoqoha 'ho hobe haholo ho ka lakatsa ho tsamaea ka thata ka lebaka la khatello kapa marotholi a eketsehang a ka hlaha kantle ho ntlha eo ho eona ho bohlokoa ho ea pelePipette Timho isa botebo bo nepahetseng.
Botebo bo khothalletsoang pakeng tsa boholo ba pipette, mofuta le ho etsoa. Litlhahiso tsa sehlahisoa sa Whist
Tatata ho ho qoelisoa ka sepheo
Boholo ba Pipette (μl) le botebo ba ho qoelisa (limilimithara)
- 1 - 100: 2 - 3
- 100 - 1,000: 2 - 4
- 1,000 - 5,000: 2 - 5
O kolota peleMalebela a Pipette
Ha liphaebu tsa pipet li kholo ho feta 10μlMalebela a PipetteHangata ho ba botsoa pele ho li tlatsa makhetlo a 'maloa ka mokelikeli o akhehileng le ho o hlakola ho senya ho ntlafatsa ho lokisa ho nepahala.
Ho sitoa ho ba koloba pele ho ka baka li-bubble tsa moea, haholo ha u sebelisa lino tse tahang kapa tsa hydrophobic kapa hydrophobic. Ho qoba li-bubble tsa moea ho netefatsa likeletso tsa pele tse metsi ha polasetiki e kholo ho feta 10μl.
Sebelisa mekhoa e feto-fetohang ea pipetting haeba ho loketse
Lintho tsee: Ho na le bothata bo tloaelehileng ha lintho tsa lipompo tsa liketsahalo tse shebahalang joalo ka lithahasello tsa protheine kapa li-acid tse 20/40
Taki butle butle, a sebelisa mokhoa o mong hape o sebelisa mokhoa oa pipetting o fokotsa menyetla ea ho thehwa bubble ha a fetisetsa tharollo ea li-Viscous.
Mokhoa oa Elisa
Phetolelo e fetotsoeng e boetse e khothalletsoa ha ho buuoa ka pipis e nyane ho96 Lifoto tsa Tlhahlobo ea Microbakeng sa mekhoa ea Elisa. Ha li-bubble tsa moea li huloa ka pipette kapa li atisa haholo ha li eketsa boleng bo ka amang melao ea letsoalo le litholoana. Ho khothalletsoa pipipting ea riverse ho khothalletsoa ho fokotsa kapa ho felisa bothata bona.
Sebelisa lipitsa tsa ERGOLIC
Likopa-pele tsa khale tse se ke tsa etsetsoa le Ergonomics ka kelellong li hloka boiteko ba matla, u khathetse 'me mokhoa oa hau oa liphaepo oa oa le mafutsana. Liphoso tse boletsoeng ka holimo ho tokollo e potlakileng ea plught e ka etsahala khafetsa.
Ka ho tsetela tharollo e matla haholoanyane u tla khona ho boloka mokhoa o motle le ho thibela litlolo tsa moea ka lebaka la mokhoa oa mafutsana.
Nka nako ea ho koetlisa basebetsi
Ho ikoetlisa khafetsa le ho lekola basebetsi ba mekhoa ea liphapang ho ka netefatsa hore phoso ea operator le sebopeho sa li-bubble se fokotsehile.
Nahana ka tharollo e boima haholoanyane
Joalokaha ho boletsoe ka holimo ho li-bubble tse ngata tsa moea li bakoa ke opereishene. E kanna ea khonahala ho fokotsa phoso ea operator le matšeliso ka ho sebelisa liphaephe tsa elektroniki kapa sethala se fetohang se nang le metsi a nang le metsi a mangata joalo kaAgilent bravo flitling roboto.
Sebelisa boleng bo botleMalebela a Pipette
Micropipettes hangata e rekoa ka tlhokomelo, empa hangata ho nahanoa ka eona e fanoa ho boleng ba ntlha ea pipette e lahliloeng. Ka lebaka la tšusumetso eo polelo e nang le ntlha e nang le eona liphethong tsa meralo, ho sebelisoa taelo e eketsehileng haeba lipeipi le malebela a tsoang ho bahlahisi ba fapaneng.
Sena e ka ba hobane malebela a theko e tlaase o ka sheba hantle empa ha u kopana le bona ka lebaka la bona ka hloko ba na le likuku, kapa ba inama kapa ba khoebetsoa.
Ho reka malebela a boleng bo botle o entsoeng ka polyproplene e phahameng ho ka fokotsa ho hlaha ha lithuthuthu tsa moea.
Ho phethela
Ho fumana li-bubble tsa moea ntlheng ea hau ea pipette e nang le katleho ea lab le ho hloka boitšoaro bo sa sebetseng hantle le litholoana tsa sephetho. Re hlokometse lintho tse 'maloa tseo u ka li etsang ho qoba li-bubble tsa moea ho kena hoPipette Tim.
Leha ho le joalo, haeba boleng bo botleMalebela a Pipetteli baka li-bubble tsa moea ho kena ntlheng ea hau ea pipette, u tla thabela ho tseba hore bokahohle ba rona bo lekaneMalebela a Pipetteli entsoe ka litekanyetso tse phahameng ka ho fetisisa 'me li entsoe ka tse hloekileng tsa Premium-li-Premium-li-Premium-Glowlene.
Khamphani ea Suzhou Acehlahisa lintlha tse 10,20,100,100,100,100,1000 le li-1250 μl. Lekhotla le ikhethang - li-racks tsohle tsa khoele kaofela li emela litlhoko tsa tšebeliso le linko tsa mulchannel. Sterlile, filthara, Rnase- / DNAS-MAHALA, le se seng morao.
Rea u amohela ho botsa ka lintlha tse ling.
Nako ea poso: la 29-2022