Sistimi e ikemetseng ea ho sebetsana le mokelikeli e na le melemo e mengata ha e sebetsana le maro a nang le bothata joalo ka lino tse nang le viscous kapa volatile, hammoho le meqolo e nyane haholo.Sistimi e na le maano a ho fana ka liphetho tse nepahetseng le tse tšepahalang ka maqheka a mang a ka lokisoang ho software.
Qalong, mokhoa oa ho sebetsana le mokelikeli o ikemetseng o ka 'na oa bonahala o le thata ebile o le boima.Empa hang ha u se u qalile ho sebetsa ka lisebelisoa tsena, u tla hlokomela hore na li nolofatsa mokhoa oa hau oa ho sebetsa joang.Baenjiniere ba thehile likarolo tse ngata tse fapaneng ho tsamaisa lits'ebetso tse thata.
Ha o sebetsana le meqolo e menyenyane ka litsamaiso tse sebetsanang le mokelikeli o ikemetseng, hoa khonahala ho lakatsa li-reagents tsohle tse hlokahalang bakeng sa karabelo e le 'ngoe.keletso, e arotsoe ke lekhalo la moea.Mokhoa ona o buisanoa haholo, haholo-holo mabapi le ho silafala ha lino tse fapaneng ka marotholi ka ntle ho metsi.ntlha ea pipette.Baetsi ba bang ba khothaletsa sena ho boloka nako le boiteko.Sistimi e ka aspirate metsi pele, lateloa ke reagent A, ebe reagent B, joalo-joalo. Mokelikeli o mong le o mong o arotsoe ka lekhalo la moea ho thibela ho kopanya kapa karabelo e qalang ka hare ho ntlha.Ha mokelikeli o ajoa, li-reagents tsohle li tsoakoa ka ho toba 'me li-volumes tse nyenyane ka ho fetisisa li hlatsuoa ho tsoakeletsoka meqolo e meholo ntlheng.Keletso e lokela ho fetoloa ka mor'a mohato o mong le o mong oa pipetting.
Khetho e betere ke ho sebelisa lisebelisoa tse khethehileng tse ntlafalitsoeng bakeng sa livolume tse nyane, mohlala, bakeng sa ho fetisa meqolo ea 1 µL ho li-jet tsa mahala.Sena se eketsa lebelo mme se qoba tšilafalo e fapaneng.Haeba li-volume tse ka tlase ho 1 µl li kentsoe ka liphaephe, ho molemo ho lahleloa ka kotloloho mokelikeli o shebiloeng kapa khahlano le bokaholimo ba sekepe ho ntša molumo kaofela.Ho fana ka liphaephe tse nyane ka ho kopana ha metsi ho boetse ho khothaletsoa ha lino tse nang le phephetso tse kang li-viscous li kenngoa ka lipeipi.
Tšobotsi e 'ngoe e thusang haholo ea litsamaiso tsa ho sebetsana le mokelikeli o ikemetseng ke ho qoelisoa ha lintlha.Ha feela 1 µL sampole e aspirated ka hokeletso, lerotholi la mokelikeli hangata le khomarela ka ntle hokeletsonakong ea ho fepa.Hoa khoneha ho hlophisa ntlha hore e kene ka har'a mokelikeli ka selibeng e le hore marotholi le marotholi a manyenyane ka ntle ho ntlha a fihlele karabelo.
Ho feta moo, ho beha aspiration le lebelo la ho fana, hammoho le bophahamo ba molumo le lebelo le tsona lia thusa.Lebelo le phethahetseng bakeng sa mofuta o mong le o mong oa mokelikeli le molumo o ka hlophisoa.'Me ho beha mekhahlelo ena ho lebisa liphellong tse ka hlahisoang haholo hobane re pipette ka lebelo le fapaneng letsatsi le leng le le leng ho itšetlehile ka tshebetso ea rona.Ho sebetsana le mokelikeli o ikemetseng ho ka nolofatsa kelello ea hau le ho eketsa ts'epo lits'ebetsong tse thata ka ho nka likarolo tse khopisang.
Nako ea poso: Feb-07-2023