U ka khetha lipoleiti le li-tubes tse nepahetseng tsa PCR joang bakeng sa ts'ebeliso ea hau?

Polymerase chain reaction (PCR) ke mokhoa o sebelisoang haholo ho baeloji ea limolek'hule bakeng sa ho holisa likhechana tsa DNA. PCR e kenyelletsa mehato e mengata, ho kenyelletsa denaturation, annealing, le katoloso. Katleho ea mokhoa ona e itšetlehile haholo ka boleng ba lipoleiti tsa PCR le li-tubes tse sebelisoang. Ho na le lintlha tse ngata tseo u lokelang ho nahana ka tsona ha u khetha lipoleiti le liphaephe tse nepahetseng tsa PCR bakeng sa kopo ea hau. Mona ke lintlha tse ling tsa bohlokoa tse lokelang ho nahanoa:

1. BokhoniLipoleiti tsa PCR'me li-tubes li tla ka boholo bo fapaneng le bokhoni. Khetho ea boholo le bokhoni bo itšetlehile haholo ka boholo ba DNA e lokelang ho ntlafatsoa ka mokhoa o le mong. Ka mohlala, haeba u hloka ho hōlisa palo e nyenyane ea DNA, u ka khetha tube e nyenyane. Haeba DNA e ngata e hloka ho holisoa, ho ka khethoa poleiti e nang le matla a maholoanyane.

2. Lisebelisoa tsa lipoleiti tsa PCR le li-tubes li ka etsoa ka lisebelisoa tse fapaneng tse kang polypropylene, polycarbonate kapa acrylic. Polypropylene ke thepa e sebelisoang ka ho fetisisa ka lebaka la lik'hemik'hale le mocheso oa eona. E boetse e theko e tlase ha e bapisoa le lisebelisoa tse ling. Li-polycarbonates le li-acrylics li theko e boima haholo, empa li na le ho hlaka ho hotle ho feta 'me li loketse PCR ea nako ea nnete.

3. Thermal conductivity PCR e kenyelletsa lipotoloho tse ngata tsa mocheso, tse hlokang ho futhumala ka potlako le ho pholisa ha motsoako oa karabelo. Ka hona, lipoleiti tsa PCR le li-tubes li tlameha ho ba le conductivity e ntle ea mocheso ho netefatsa ho futhumala ho tšoanang le ho pholile ha motsoako oa karabelo. Lipoleiti tse nang le marako a masesaane le libaka tse bataletseng li loketse ho eketsa mocheso o fetisang mocheso.

4. Ho lumellana ha lipoleiti tsa PCR le li-tubes li lokela ho lumellana le sebaesekele sa mocheso seo u se sebelisang. Lipoleiti le li-tubes li tlameha ho khona ho mamella mocheso o phahameng o hlokahalang bakeng sa ho hōlisa likaroloana tsa DNA. Kamehla buisana le moetsi oa libaesekele tse futhumatsang bakeng sa lipoleiti le liphaephe tse khothalelitsoeng.

5. Tiiso Tiiso e tiileng e bohlokoa ho thibela tšilafalo ea motsoako oa karabelo. Lipoleiti tsa PCR le li-tubes li ka koaloa ho sebelisoa mekhoa e fapaneng e kang litiiso tsa mocheso, lifilimi tse khomarelang kapa li-lids. Ho tiisa mocheso ke mokhoa o sireletsehileng ka ho fetisisa 'me o fana ka thibelo e matla khahlanong le tšilafalo.

6. Sterilization PCR lipoleiti le li-tubes li tlameha ho se be le litšila tse ka sitisang maikutlo. Ka hona, li tlameha ho hloekisoa pele li sebelisoa. Ho bohlokoa ho khetha lipoleiti le li-tubes tse bonolo ho thibela likokoana-hloko le mekhoa ea ho thibela mocheso ka lik'hemik'hale le mocheso.

Ka kakaretso, ho khetha poleiti e nepahetseng ea PCR le li-tubes ho bohlokoa bakeng sa ho holisa DNA ka katleho. Khetho e itšetlehile haholo ka mofuta oa kopo, palo ea DNA e holisitsoeng, le ho lumellana le libaesekele tsa mocheso.

Suzhou Ace Biomedical Technology Co., Ltd. e fana ka mefuta e mengata ea lipoleiti tsa boleng bo holimo tsa PCR le li-tubes ka boholo bo fapaneng, bokhoni le lisebelisoa ho finyella litlhoko tsa mofuputsi e mong le e mong.