Waa maxay saxan PCR?

Saxanka PCR waa nooc ka mid ah furayaasha, dNTP, Taq DNA polymerase, Mg, template nucleic acid, baffer iyo sideyaal kale oo ku lug leh falcelinta xoojinta silsiladda Polymerase Reaction (PCR).

1. Isticmaalka saxanka PCR

Waxaa si weyn loogu isticmaalaa dhinacyada hidda-socodka, biochemistry, difaaca, daawada, iwm, ma aha oo kaliya cilmi-baarista aasaasiga ah sida go'doominta hidda-socodka, cloning iyo falanqaynta isku xigxiga aashitada nucleic, laakiin sidoo kale ogaanshaha cudurrada ama meel kasta oo ay jirto DNA. iyo RNA. Waa wax hal mar lagu isticmaalo shaybaarka. Alaabta

2.96 Waa hagaag PCRWalax saxan ah

Walxaha u gaarka ah ayaa ah inta badan polypropylene (PP) maalmahan, si ay si fiican ula qabsan karto goobaha heerkulka sare iyo hoose ee soo noqnoqda ee habka falcelinta PCR, oo ay ku gaari karto heerkul sare iyo nadiifin cadaadis sarreeya. Si loo gaaro hawlgal heersare ah oo ay weheliso qori isku xigta, mashiinka PCR, iwm, 96-well ama 384-ceel PCR ayaa inta badan la isticmaalaa. Qaabka saxanku wuxuu u hoggaansamaa heerka caalamiga ah ee SBS, iyo si loo waafajiyo mashiinnada PCR ee soo saarayaasha kala duwan, waxaa loo qaybin karaa afar qaab oo naqshadeysan: maro maro, maro badhkeed, goonno kor loo qaaday iyo goonno buuxda sida waafaqsan naqshadaynta.

3. Midabka ugu weyn ee saxanka PCR

Kuwa caadiga ah waa cadcad iyo caddaan, kuwaas oo taarikada PCR-yada cad ay aad ugu habboon yihiin PCR-ga tirooyinka-floorescent-ka ee waqtiga-dhabta ah.