Cryovialswaxaa caadi ahaan loo isticmaalaa kaydinta cryogenic ee xadhkaha unugyada iyo walxaha kale ee bayooloji ee muhiimka ah, ee dewars ay ka buuxaan nitrogen dareere ah.

Waxaa jira dhowr marxaladood oo ku lug leh ilaalinta guusha leh ee unugyada ku jira nitrogen dareeraha ah. Inkasta oo mabda'a aasaasiga ah uu yahay baraf tartiib tartiib ah, farsamada saxda ah ee la isticmaalo waxay ku xiran tahay nooca unugga iyo cryoprotectant la isticmaalo. Waxaa jira dhowr tixgalin badbaado iyo hababka ugu wanaagsan ee lagu xisaabtamayo marka lagu kaydinayo unugyada heerkulka hooseeya.

Boostada waxay ujeedadeedu tahay inay bixiso dulmar ku saabsan sida cryovials loogu kaydiyo nitrogen dareere ah.

Waa maxay Cryovials

Cryovials waa dhalooyin yaryar oo daboolan oo loogu talagalay in lagu kaydiyo shaybaarada dareeraha ah heerkul aad u hooseeya. Waxay hubiyaan in unugyada lagu ilaaliyo cryoprotectant aysan si toos ah ula xiriirin nitrogen dareeraha ah, iyagoo yareynaya halista jabka gacanta iyadoo weli ka faa'iideysanaya saameynta qaboojinta ba'an ee nitrogen dareeraha ah.

Fijaanadaha ayaa inta badan lagu heli karaa tirooyin iyo naqshado kala duwan - waxay noqon karaan kuwo gudaha ama dibadda ah oo lagu dhejiyo dabaq siman ama wareegsan. Qaabab aan nadiif ahayn iyo kuwa aan nadiif ahayn ayaa sidoo kale la heli karaa.

Yaa IsticmaalaCyrovialsIn lagu kaydiyo Unugyada Nitrojiinka dareeraha ah

Noocyo kala duwan oo NHS ah iyo shaybaarrada gaarka ah, iyo sidoo kale machadyada cilmi-baarista ee ku takhasusay bangiyada dhiigga ee xad-dhaafka ah, bayoolaji unugyada unugyada epithelial, immunology iyo bayooloji unugyada stem waxay isticmaalaan cryovials si ay u ilaaliyaan unugyada.

Unugyada habkan loo ilaaliyo waxaa ka mid ah Unugyada B iyo T, Unugyada CHO, Unugyada Hematopoietic Stem iyo Progenitor Unugyada, Hybridomas, Unugyada Mindhicirka, Macrophages, Stem Mesenchymal iyo Unugyada Progenitor, Monocytes, Myeloma, Unugyada NK iyo Unugyada stem Pluripotent.

Dulmarka Sida loo kaydiyo Cryovials ee dareeraha Nitrogen

Cryopreservation waa nidaam ilaalinaya unugyada iyo dhismooyinka kale ee noolaha iyadoo lagu qaboojinayo heerkul aad u hooseeya. Unugyada waxa lagu kaydin karaa nitrogen dareere ah sannado iyada oo aan la lumin awoodda unugyada. Tani waa dulmar habraacyada la adeegsaday.

Diyaarinta Unugyada

Habka saxda ah ee loo diyaariyo muunada way kala duwanaan doontaa iyadoo ku xidhan nooca unugga, laakiin guud ahaan, unugyada waa la ururiyaa oo la xoojiyaa si loo horumariyo pellet-ka hodanka ah. Pellet-kan ayaa markaa dib loogu soo celinayaa korka sare ee lagu qasay cryoprotectant ama dhexdhexaadiyaha ilaalinta.

Dhexdhexaadiyaha Cryopreservation

Dhexdhexaadintan waxaa loo shaqeeyaa si ay u ilaaliso unugyada jawiga heerkulka hooseeya ee lagu hoos gelin doono iyada oo la joojinayo samaynta crystals intra iyo extracellular iyo markaa dhimashada unugyada. Doorkoodu waa inay bixiyaan deegaan badbaado leh oo ilaalinaya unugyada iyo unugyada inta lagu jiro habka qaboojinta, kaydinta, iyo dhalaalidda.

Dhexdhexaad ah sida balaasmaha barafaysan ee cusub (FFP), xalka plasmalyte heparinised ama serum-free, xalalka xoolaha ka xorta ah waxaa lagu qasaa cryoprotectants sida dimethyl sulphoxide (DMSO) ama glycerol.

Pellet muunada dib loo shubay waxa lagu shubaa kriyovials polypropylene sidaSuzhou Ace Biomedical Shirkadda Cryogenic Storage Vials.

Waxaa muhiim ah in aan la buuxin cryovials sababtoo ah tani waxay kordhin doontaa khatarta dillaaca iyo sii deynta suurtogalka ah ee ku jira (1).

Heerka Barafka La Xakameeyay

Guud ahaan, heerka qaboojinta qunyar-socodka ah ee la kantaroolo ayaa loo shaqeeyaa ilaalinta guusha ee unugyada.

Ka dib markii shaybaarada lagu shubo fiilooyinka cryogenic, waxaa lagu dhejiyaa baraf qoyan ama qaboojiyaha 4 ℃ waxaana habka qaboojinta la bilaabayaa 5 daqiiqo gudahood. Tilmaamaha guud ahaan, unugyada waxaa lagu qaboojiyaa heer -1 ilaa -3 daqiiqaddii (2). Tan waxa lagu gaaraa iyada oo la isticmaalayo qaboojiye barnaamijaysan ama in fijaanno la geliyo sanduuq dahaaran oo lagu meeleeyay qaboojiyaha heerka -70°C ilaa -90°C.

U wareeji Nitrogen dareere ah

Fijaannada la qaboojiyey ayaa markaa lagu wareejiyaa haanta nitrogen ee dareeraha ah muddo aan xad lahayn haddii heerkul ka yar -135 ℃ la ilaaliyo.

Heerkulkan aadka u hooseeya waxaa lagu heli karaa dhexgalka dareeraha ama wajiga uumiga nitrogen.

Dareeraha ama Wajiga Uumiga?

Kaydinta marxaladda dareeraha ah ee nitrogen waxaa loo yaqaanaa inay ilaaliso heerkulka qabowga oo leh joogteyn buuxda, laakiin inta badan laguma talinayo sababaha soo socda:

• Baahida loo qabo qiyaaso waaweyn (qoto dheer) nitrogen dareere ah taas oo ah khatar iman karta. Gubasho ama neef-qabatin taas awgeed waa khatar dhab ah.
• Kiisaska la diiwaan galiyay ee wasakhaynta isdhaafsiga ee wakiilada faafa sida aspergillus, hep B iyo fayraska ayaa ku faafaya dhexdhexaadiyaha nitrogen ee dareeraha ah (2,3)
• Suurtagalnimada in nitrogen dareere ah ay ku daadato dhalooyinka inta lagu jiro dhexgalka. Marka laga saaro kaydinta oo lagu diiriyo heerkulka qolka, nitrogen-ku si degdeg ah ayuu u fidaa. Sidaa awgeed, fijaanku waxa laga yaabaa inuu burburo marka laga saaro kaydinta nitrogen ee dareeraha ah, taasoo abuuraysa khatar ka timaada burburka duulaya iyo soo-gaadhista waxa ku jira (1, 4).

Sababahan dartood, kaydinta heerkulka aadka u hooseeya ayaa inta badan ku jira wajiga uumiga nitrogen. Marka shaybaarada ay tahay in lagu kaydiyo marxaladda dareeraha ah, tuubooyinka cryoflex gaar ah waa in la isticmaalaa.

Hoos-u-dhaca wejiga uumiga ayaa ah in heer-kulka toosan uu dhici karo taasoo keentay isbedbeddelka heerkulka u dhexeeya -135 ℃ iyo -190 ℃. Tani waxay u baahan tahay in si taxadar leh oo taxadar leh loola socdo heerarka nitrogen ee dareeraha ah iyo kala duwanaanta heerkulka (5).

Soosaarayaal badan ayaa ku taliya in cryovials ay ku habboon yihiin kaydinta ilaa -135 ℃ ama loo isticmaalo wajiga uumiga oo keliya.

Dhalalinaya Unugyadaada La Ilaaliyay

Habka dhalaalintu waxa ay culays ku haysaa dhaqanka barafoobay, waxaana loo baahan yahay in si habboon loo maareeyo iyo farsamada si loo hubiyo jiritaanka ugu habboon, soo kabashada, iyo shaqaynta unugyada. Habka dhalaalinta saxda ah waxay ku xirnaan doontaa noocyada unugyada gaarka ah. Si kastaba ha ahaatee, dhalaalid degdeg ah ayaa loo tixgaliyaa heerka si:

• Iska yaree saameyn kasta oo ku saabsan soo kabashada gacanta
• Ka caawi in la yareeyo wakhtiga soo-gaadhista ee solutes ee ku jira warbaahinta qaboojinta
• Iska yaree waxyeelada dib-u-krishadaynta barafka

Qubeyska biyaha, musqulaha qubeyska, ama aaladaha otomaatiga ah ee khaaska ah ayaa caadi ahaan loo isticmaalaa in lagu dhalaaliyo shaybaarada.

Inta badan 1 xariiq unug ayaa la dhalaaliyaa hal mar 1-2 daqiiqo, iyadoo si tartiib ah loogu wareego 37 ℃ qubeyska biyaha ilaa ay ka jirto wax yar oo baraf ah oo ku haray fiishka ka hor inta aan lagu dhaqin dhexdhexaad koritaan ah oo horay loo diiray.

Unugyada qaarkood sida embriyaha naasleyda ah, kulaylka tartiib-tartiib ah ayaa muhiim u ah badbaadadooda.

Unugyadu hadda waxay diyaar u yihiin dhaqanka unugga, go'doominta unugga, ama xaaladda unugyada tarma ee hematopoietic - daraasaadka suurtagalka ah si loo dammaanad qaado daacadnimada unugyada tarma ee deeqaha ka hor daaweynta myeloablative.

Waa caado caadi ah in la qaato aliquots yar yar ee muunadda hore loo dhaqay ee loo isticmaalo in lagu sameeyo tirada unugyada si loo go'aamiyo uruurinta unugga ee ku dhejinta dhaqanka. Waxaad markaas qiimeyn kartaa natiijooyinka hababka go'doominta unugga oo waxaad go'aamin kartaa jiritaanka unugga.

Hababka ugu Fiican ee Kaydinta Cryovials

Kaydinta guusha ee shaybaarada lagu kaydiyo cryovials waxay ku xidhan tahay waxyaabo badan oo borotokoolka ku jira oo ay ku jiraan kaydinta saxda ah iyo kaydinta diiwaanka.

• Unugyada u kala saar meelaha kaydinta- Haddii xajmigu oggolaado, u kala qaybi unugyada fijaanadaha oo ku kaydi meelo gaar ah si loo yareeyo khatarta muunad luminta qalabka cilladaysan awgeed.
• Kahortagga faddaraynta- Dooro hal-isticmaalka fijaannada nadiifka ah ee cryogenic ama autoclave ka hor isticmaalka dambe
• U isticmaal unugyadaada fijaanno si habboon u cabbiranFijaanadu waxay ku yimaadaan qiyaaso kala duwan oo u dhexeeya 1 iyo 5mls. Iska ilaali inaad si xad dhaaf ah u buuxisid fijaanadaha si aad u yarayso khatarta dildilaaca.
• Dooro fiilooyinka cryogenic ee gudaha ama dibadda dunta ka sameysanFijaannada dunta gudaha ah ayaa jaamacadaha qaarkood kula taliyaan tallaabooyinka badbaadada - waxay sidoo kale ka hortagi karaan faddaraynta marka la buuxinayo ama marka lagu kaydiyo nitrogen dareeraha ah.
• Kahortagga daadinta-Isticmaal shaabad laba-durid ah oo lagu shubay koofiyada furka ama O-rings si aad uga hortagto daadinta iyo wasakheynta.
• Isticmaal koodhadhka 2D oo ku calaamadee fijaannada- si loo hubiyo in la raadiyo, fijaanno leh meelo waaweyn oo wax lagu qoro ayaa awood u siinaya fijaan kasta in lagu calaamadiyo ku filan. Barcode 2D ayaa kaa caawin kara maaraynta kaydinta iyo kaydinta diiwaanka. Koofiyadaha summada leh ayaa faa'iido u leh aqoonsiga fudud.
• Dayactirka kaydinta ku filan- Si loo hubiyo in unugyada aan la lumin, weelasha kaydinta waa inay si joogto ah ula socdaan heerkulka iyo heerarka nitrogen dareeraha ah. Alaarmiga waa in lagu rakibaa si looga digtoonaado isticmaalayaasha khaladaadka.

Taxadirka Badbaadada

Nitrojiin dareeraha ah wuxuu noqday caado caadi ah cilmi-baarista casriga ah laakiin waxay wadataa halista dhaawac halis ah haddii si khaldan loo isticmaalo.

Qalabka ilaalinta shakhsi ahaaneed ee saxda ah (PPE) waa in la gashado si loo yareeyo khatarta dhaxan, gubashada iyo dhacdooyinka kale ee xun marka la xakameynayo nitrogen dareeraha ah. Xiro

• galoofyada Cryogenic
• Jaakad shaybaadhka
• Saamaynta difaac buuxda oo wejiga u adkaysata oo waliba daboosha qoorta
• Kabaha lugaha xiran
• Maro caag ah oo aan nadiif ahayn

Talaagadaha nitrogen ee dareeraha ah waa in la dhigaa meelaha hawo fiican leh si loo yareeyo khatarta neefta - nitrogen ka baxsatay ayaa soo baxsa oo barata ogsijiin atmospheric. Dukaamada mugga waaweyn waa inay lahaadaan nidaamyada digniinta ogsijiinta hoose.

U shaqaynta laba-labo marka la maaraynayo nitrogen dareeraha ah ayaa ku habboon isticmaalkeeda wax ka baxsan saacadaha shaqada ee caadiga ah waa in la mamnuucaa.

Cryovials si ay u taageeraan socodkaaga shaqada

Shirkadda Suzhou Ace Biomedical waxay bixisaa xulasho ballaaran oo alaabooyin ah oo buuxinaya baahiyahaaga ilaalinta ee noocyada kala duwan ee unugyada. Portfolio-ga waxa ku jira tubo kala duwan iyo sidoo kale noocyo kala duwan oo ah cryovials oo nadiif ah.

Cryovials our waa:

• Daboolka Shaybaadhka 0.5ml 1.5ml 2.0ml Cryovial Cryogenic Vials Conical Bottom Cryotube oo leh Gasket

  ● 0.5ml,1.5ml,2.0ml qeexid,oo leh goono ama maran la'aan
  ● Naqshad toosan ama mid iskeed u taagan, nadiif ah ama aan nadiif ahayn labadaba waa la heli karaa
  ● Tuubooyinka koofiyadaha waxay ka samaysan yihiin polypropylene heer caafimaad
  PP Cryotube Vials si isdaba joog ah ayaa loo qaboojin karaa waana la dhalaali karaa
  ●Naqshadda koofiyadda dibadda waxay yarayn kartaa ixtimaalka faddaraynta inta lagu jiro daawaynta muunadda.
  ● Fur dabool tuubooyinka cryogenic-ka dunta caaga ah ee la isticmaalo
  ● Tuubooyinka waxay ku haboon yihiin rotors-ka caadiga ah
  ● Tuubooyinka Cryogenic tuubooyinka o-ring waxay ku habboon yihiin heerka 1-inch iyo 2-inch, 48well, 81well,96well iyo 100well
  ● Autoclavable ilaa 121°C waana la qaboojin karaa ilaa -86°C

  QAYBTA NO	ALAAB	CODKA	CAPMIDABKA	PCS/BAG	Bacaha/CASE
  ACT05-BL-N	PP	0.5ML	Madow, Jaalle, Buluug, Cas, Purple, White	500	10
  ACT15-BL-N	PP	1.5ML	Madow, Jaalle, Buluug, Cas, Purple, White	500	10
  ACT15-BL-NW	PP	1.5ML	Madow, Jaalle, Buluug, Cas, Purple, White	500	10
  ACT20-BL-N	PP	2.0ML	Madow, Jaalle, Buluug, Cas, Purple, White	500	10