Fal-celinta silsiladda polymerase (PCR) waa farsamo si weyn loo isticmaalo bayooloojiyada molecular si loo kordhiyo jajabyada DNA-da. PCR waxay ku lug leedahay dhowr tillaabo, oo ay ku jiraan denaturation, annealing, iyo fidinta. Guusha farsamadani waxay inta badan ku xidhan tahay tayada taarikada PCR iyo tuubooyinka la isticmaalo. Waxa jira arrimo badan oo ay tahay in la tixgeliyo marka la dooranayo taarikada PCR-ga iyo tuubooyinka ku habboon codsigaaga. Waa kuwan arrimo muhiim ah oo ay tahay in la tixgeliyo:
1. AwoodTaarikada PCRtuubooyinku waxay ku yimaadaan cabbir iyo awoodo kala duwan. Doorashada cabbirka iyo awoodda waxay inta badan ku xiran tahay qaddarka DNA-da ee u baahan in lagu kordhiyo hal fal-celin. Tusaale ahaan, haddii aad u baahan tahay inaad kordhiso qadar yar oo DNA ah, waxaad dooran kartaa tuubo yar. Haddii tiro badan oo DNA ah loo baahan yahay in la kordhiyo, saxan leh awood weyn ayaa la dooran karaa.
2. Qalabka PCR iyo tuubooyinka waxaa laga samayn karaa walxo kala duwan sida polypropylene, polycarbonate ama akril. Polypropylene waa walxaha inta badan la isticmaalo sababtoo ah caabbinta kiimikada iyo kulaylka. Sidoo kale way ka jaban tahay marka loo eego alaabta kale. Polycarbonates iyo acrylics aad bay qaali u yihiin, laakiin waxay leeyihiin hufnaan muuqaal oo wanaagsan waxayna ku habboon yihiin PCR-ga-waqtiga-dhabta ah.
3. Kulaylka PCR waxay ku lug leedahay wareegyo badan oo kulayl ah, oo u baahan kuleyl degdeg ah iyo qaboojinta isku darka falcelinta. Sidaa darteed, taarikada PCR iyo tuubooyinka waa inay lahaadaan kuleyl wanaagsan si loo hubiyo kululaynta isku midka ah iyo qaboojinta isku darka falcelinta. Taarikada derbiyada dhuuban iyo sagxadaha fidsan ayaa ku habboon kor u qaadista kulaylka.
4. Taarikada PCR iyo tuubooyinka waafaqayaan waa in ay la jaanqaadaan wareegga kulaylka ee aad isticmaalayso. Taarikada iyo tuubooyinka waa in ay u adkeysan karaan heerkulka sare ee looga baahan yahay in lagu kordhiyo jajabyada DNA-ga. Had iyo jeer kala tasho soo saaraha kuleyliyaha taarikada iyo tuubooyinka lagu taliyey.
5. Xidh shaabad adag ayaa muhiim ah si looga hortago faddaraynta isku darka falcelinta. Taarikada PCR iyo tuubooyinka waxaa lagu xiri karaa habab kala duwan sida xirmooyinka kulaylka, filimada xabagta ama daboolka. Xiritaanka kulaylku waa habka ugu badbaadsan waxayna siisaa caqabad adag oo ka hortagaysa faddaraynta.
6. Madhalayska taarikada PCR iyo tuubooyinka waa inay ka nadiif yihiin wax kasta oo wasakhaysan oo faragelin kara falcelinta. Sidaa darteed, waa in la nadiifiyaa kahor isticmaalka. Waa muhiim in la doorto taarikada iyo tuubooyinka si sahlan loo nadiifiyo oo u adkaysta hababka jeermiska kiimikada iyo kulaylka.
Marka la soo koobo, doorashada saxanka PCR ee saxda ah iyo tuubooyinka ayaa muhiim u ah kobcinta DNA-da ee guusha leh. Doorashadu waxay inta badan ku xiran tahay nooca codsiga, qadarka DNA-ga la xoojiyay, iyo waafaqsanaanta wareegyada kulaylka.
Suzhou Ace Biomedical Technology Co., Ltd. Waxay bixisaa taargooyin PCR iyo tuubooyin tayo sare leh oo kala duwan oo cabbirro kala duwan leh, awood iyo qalab ah si loo daboolo baahiyaha cilmi-baare kasta.
Waqtiga boostada: Meey-17-2023