Pipette ndechimwe chezvishandiso zvinonyanya kushandiswa mumarabhoritari ebhayoloji, kiriniki, uye analytical uko zvinwiwa zvinoda kuyerwa nemazvo nekufambiswa pakuita dilution, assays kana ongororo yeropa. Anowanikwa se:
① single-channel kana akawanda-channel
② vhoriyamu yakagadziriswa kana inogadziriswa
③ zvemanyorero kana zvemagetsi
Chii chinonzi Single-Channel Pipettes?
Iyo single-channel pipette inobvumira vashandisi kutamisa imwe aliquot panguva. Izvi zvinowanzo shandiswa mumarabhoritari ane mashoma ekubuda kwemasamples, ayo anogona kazhinji kuve aya anobatanidzwa mukutsvaga nekusimudzira.
Iyo single-channel pipette ine musoro mumwechete wekushuvira kana kuburitsa mazinga chaiwo emvura kuburikidza neinoraswa.tip. Iwo anogona kushandiswa kune akawanda maapplication mukati memarabhoritari ayo anongove nediki throughput. Aya kazhinji ari marabhoritari anoita tsvakiridzo ine chekuita neanalytical chemistry, cell tsika, genetics kana immunology.
Chii chinonzi Multi-Channel Pipettes?
Multi-channel pipettes inoshanda nenzira imwechete se-single-channel pipettes, asi inoshandisa akawandamazanopakuyera uye kugovera huwandu hwakaenzana hwemvura kamwechete. Common setups 8 kana gumi nemaviri chiteshi asi 4, 6, 16 uye 48 chiteshi seti dziripowo. 96 chiteshi benchtop shanduro dzinogona kutengwa zvakare.
Uchishandisa multi-channel pipette, zviri nyore kukurumidza kuzadza 96-, 384-, kana 1,536-tsime.microtiter ndiro, iyo inogona kunge iine sampuli dzemashandisirwo akadai seDNA amplification, ELISA (diagnostic test), kinetic zvidzidzo uye kuongorora mamorekuru.
Single-Channel vs. Multi-Channel Pipettes
Kubudirira
Iyo single-channel pipette yakanaka kana uchiita basa rekuyedza. Izvi zvinodaro nekuti zvinongo sanganisira kushandisa machubhu ega ega, kana imwe muchinjikwa-match kuita mukuwedzerwa ropa.
Nekudaro, izvi zvinokurumidza kuve chishandiso chisingashande kana throughput ichiwedzerwa. Kana paine akawanda masampuli / reagents ekufambisa, kana maassays akakura ari kuitwa mukati96 zvakanaka microtitre mahwendefa, kune nzira yakanyatsoshanda yekufambisa zvinwiwa ipapo uchishandisa imwe-chaneli pipette. Nekushandisa ma-multi-channel pipette pane kudaro, nhamba yepipetting matanho inoderedzwa zvinoshamisa.
Tafura iri pazasi inoratidza huwandu hwematanho ekupopa anodiwa kune imwechete-chaneli, 8 uye 12 chiteshi setups.
Nhamba yematanho epipetting anodiwa (6 reagents x96 Zvakanaka Microtitre Plate)
Imwe-chiteshi Pipette: 576
8-Channel Pipette: 72
12-Channel Pipette: 48
Kuwanda kwePipetting
Imwe misiyano yakakosha pakati peiyo imwe uye yakawanda-channel pipettes ivhoriyamu patsime rinogona kutamiswa panguva imwe chete. Kunyangwe zvinoenderana nemuenzaniso uri kushandiswa, kazhinji haugone kutamisa yakawandisa vhoriyamu nemusoro pane yakawanda-channel pipette.
Iyo vhoriyamu imwe-channel pipette inogona kutamisa mitsara pakati pe0.1ul uye 10,000ul, uko huwandu hweakawanda-channel pipette iri pakati pe0.2 ne1200ul.
Muenzaniso Loading
Nhoroondo, mapipette e-multi-channel anga asina kusimba uye akaoma kushandisa. Izvi zvakonzera kusawirirana kurodha, pamwe nekunetseka kurodhamazano. Kune mhando nyowani dziripo iye zvino, idzo dziri mushandisi-hushamwari uye dzinoenda neimwe nzira yekugadzirisa nyaya idzi. Izvo zvakakoshawo kuziva kuti kunyangwe kurodha kwemvura kungave kushoma zvishoma ne-multi-channel pipette, ivo vanonyanya kuve vakanyanya kurongeka pane imwechete-channel nekuda kwekukanganisa kunoitika kubva mukukanganisa kwemushandisi nekuda kwekuneta. ona ndima inotevera).
Kuderedza Kukanganisa Kwevanhu
Mukana wekukanganisa kwevanhu unodzikiswa zvakanyanya sezvo huwandu hwematanho epombi hunodzikira. Kusiyana kubva kuneta uye kufinhwa kunobviswa, zvichikonzera data nemhedzisiro inovimbika uye inogoneka.
Calibration
Kuti ive nechokwadi chechokwadi uye nemazvo emidziyo yekubata mvura, kugaro gadzirisa kunodiwa. Standard ISO8655 inotaura kuti chiteshi chega chega chinofanirwa kuongororwa uye kutaurwa. Iyo yakawanda nzira iyo pipette ine, iyo inotora nguva yakareba kugadzirisa iyo inogona kutora nguva.
Zvinoenderana nepipettecalibration.net chiyero che 2.2 calibration pane 12-channel pipette inoda 48 pipetting cycles uye gravimetric huremu (2 mavhoriyamu x 2 repetitions x 12 chiteshi). Zvichienderana nekumhanya kwemushandisi, izvi zvinogona kutora maawa anopfuura 1.5 papipette. Marabhoritari muUnited Kingdom anoda kuti UKAS calibration yaizoda kuita huremu hwe360 gravimetric huremu (3 mavhoriyamu x 10 repetitions x 12 chiteshi). Kuita nhamba iyi yebvunzo pamaoko hazvigoneke uye zvinogona kudarika nguva yekuchengetedza inowanikwa nekushandisa yakawanda-channel pipette mune mamwe maLab.
Nekudaro, kukunda matambudziko aya pipette calibration masevhisi anowanikwa kubva kumakambani akati wandei. Mienzaniso yeiyi Gilson Labs, ThermoFisher uye Pipette Lab.
Kugadzirisa
Haisi chinhu icho vazhinji vanofunga nezvazvo pavanenge vachitenga pipette itsva, asi kuwanda kwemamwe ma-multi-channel pipettes haagadziriswi. Izvi zvinoreva kana 1 chiteshi chakuvadzwa, iyo manifold yese inogona kuchinjwa. Nekudaro, vamwe vagadziri vanotengesa zvinotsiva ega ega, saka iva nechokwadi chekutarisa kugadzirisa nemugadziri kana uchitenga akawanda-channel pipette.
Pfupiso - Single vs Multi-Channel Pipettes
Iyo yakawanda-channel pipette chishandiso chakakosha cherabhoritari yega yega ine chero chinhu chinopfuura kudiki-diki kuburitsa kwemasampuli. Munenge mune yega yega mamiriro ehuwandu hwehuwandu hwemvura inodiwa pakufambisa iri mukati mekukwanisa kweimwe neimwetippane yakawanda-channel pipette, uye pane zvishoma zvipingamupinyi zvine chokuita neizvi. Chero kuwedzera kudiki kwekuoma mukushandisa yakawanda-channel pipette yakanyanya kuremerwa nekuderera kwemambure mubasa rekushanda, inogoneswa nehuwandu hwakaderedzwa hwematanho epipetting. Zvese izvi zvinoreva kuvandudzwa kwemushandisi kunyaradzwa, uye kudzikira kukanganisa kwemushandisi.
Nguva yekutumira: Zvita-16-2022