Sei Murabhoritari Consumables Inodikanwa kuve DNase uye RNase Yemahara?

Mumunda wemolecular biology, huchokwadi uye kuvimbika kwakakosha zvakanyanya. Chero kusvibiswa kwezvinhu zvinoshandiswa murabhoritari zvinogona kutungamirira kumigumisiro isina kururama, iyo inogona kuva nemigumisiro yakakomba yekutsvakurudza kwesayenzi uye kuongororwa. Imwe inowanzoitika yekusvibiswa kuvepo kweDNase uye RNase enzymes. Aya ma enzymes anokanganisa DNA neRNA, zvichiteerana, uye anogona kuwanikwa mune akasiyana biological matrices. Kudzikamisa njodzi yekusvibiswa uye kuve nechokwadi chemhedzisiro, zvigadzirwa zvemurabhoritari, sengepipette mazano, ndiro dzakadzika dzetsime, PCR mahwendefa, uye machubhu, inofanira kunge iri DNase uye RNase yemahara.

DNase uye RNase enzymes anowanikwa kwese uye anogona kuwanikwa munzvimbo dzakasiyana siyana dzehupenyu, kusanganisira muviri wemunhu, zvidyarwa, uye tupukanana. Ivo vanotamba mabasa akakosha mumaserura maitiro senge DNA kupatsanuka, kugadzirisa kweDNA, uye kuparara kweRNA. Nekudaro, kuvepo kwavo munzvimbo yemurabhoritari kunogona kukanganisa kuedza kunosanganisira kuongororwa kweDNA neRNA.

Pipette matipi ndechimwe chezvinhu zvinonyanya kushandiswa murabhoritari. Iwo anoshandiswa pakubata kwemvura kwakaringana uye kwakaringana, zvichiita kuti ive yakakosha kune akasiyana maapplication akadai sekugadzirira sampuli, DNA kutevedzana, uye PCR. Kana matipi epipette asiri DNase uye RNase akasununguka, kusvibiswa kunogona kuitika panguva yepipetting, zvichitungamira mukuderedzwa kweDNA kana RNA samples. Izvi zvinogona kuguma nemigumisiro yakaipa yenhema kana isina kukwana, kukanganisa kuvimbika kwekuedza kwose.

Mahwendefa etsime rakadzika ndeimwe yakakosha marabhoritari inodyiwa, kunyanya mumashandisirwo epamusoro-soro. Iwo anoshandiswa kune sampuli yekuchengetedza, serial dilutions, uye sero tsika. Kana mahwendefa aya asina DNase uye RNase akasununguka, chero DNA kana RNA samples dzakachengetwa mairi dzinogona kusvibiswa, zvichikonzera kuparara kweiyo nucleic acids. Izvi zvinogona kukanganisa kurongeka kwezvishandiso zvepasi sePCR, qPCR, kana kutevedzana kwechizvarwa chinotevera.

Saizvozvo, PCR mahwendefa uye machubhu zvinhu zvakakosha mu polymerase chain reaction (PCR) application. PCR inzira inoshandiswa zvakanyanya yekukudza DNA sequences. Kana PCR mahwendefa nemachubhu zvakasvibiswa neDNase kana RNase, nzira yekusimudzira inogona kukanganisa, zvichiita kuti zvive nemigumisiro isina kururama uye kududzira kwenhema. DNase uye RNase-isina PCR zvinoshandiswa zvinodzivirira kushatiswa kweinonangwa DNA kana RNA panguva yekusimudzira maitiro, kuve nechokwadi chekuvimbika uye kuberekazve.

Kugadzirisa nyaya yekusvibiswa, zvinoshandiswa murabhoritari zvinoda kugadzirwa nemaitiro anodzorwa zvakanyanya uye zvinhu zvakasimbiswa kuve DNase uye RNase yemahara. Makambani akaita seSuzhou Ace Biomedical Technology Co., Ltd., ane hunyanzvi mukugadzira zvinhu zvemurabhoritari zvinosangana nezvinodiwa zvakasimba izvi. Semugadziri anotungamira mumunda, Suzhou Ace Biomedical Technology Co., Ltd. inokoshesa kunaka uye kuvimbika.

Suzhou Ace Biomedical Technology Co., Ltd. inonzwisisa kukosha kweiyo DNase uye RNase kusvibiswa mune zvinodyiwa murabhoritari. Mazano avo epipette, mahwendefa akadzika, mahwendefa ePCR, uye machubhu zvese zvinogadzirwa zvichishandiswa zvemhando yepamusoro izvo zvinotora matanho ekudzora emhando yepamusoro kuti ave nechokwadi chekuti DNase uye RNase yemahara.

Iyo kambani inoshandisa hunyanzvi hwekugadzira epamberi uye inoomerera kune akasimba emhando zviyero kubvisa njodzi yekusvibiswa, nekudaro ichivimbisa mhedzisiro uye yakavimbika mhedzisiro yevaongorori nevarapi zvakafanana. Ivo vanonzwisisa kuti chero kukanganisa mumhando yezvigadzirwa zvemurabhoritari zvinogona kuve nemhedzisiro inosvika kure, kwete mukutsvagisa chete asiwo mumakiriniki ekushandisa apo kuongororwa kwakakosha kwakakosha.

Mukupedzisa, zvinoshandiswa murabhoritari senge matipi epipette, mahwendefa emutsime akadzika, mahwendefa ePCR, uye machubhu anofanirwa kunge ari DNase uye RNase akasununguka kuti ave nechokwadi chechokwadi uye kuvimbika kwekuyedzwa kwema molecular biology. Kusvibiswa nema enzymes aya kunogona kutungamirira mukuparara kweDNA neRNA samples, kukanganisa kutendeseka kwemigumisiro yakawanikwa. Makambani akadaiIyo kambani Suzhou Ace Biomedical Technology Co., Ltd. kunzwisisa kukosha kwekugadzira zvigadzirwa zvinosangana nezvinodiwa izvi zvakasimba, zvichiita kuti masayendisiti nevarapi vaite basa ravo nechivimbo uye nemazvo.