Kuti uwane budiriro yekusimudzira maitiro, zvinodikanwa kuti izvo zvega zvega zvimiro zvivepo mukusangana chaiko mugadziriro yega yega. Mukuwedzera, zvakakosha kuti hapana kusvibiswa kunoitika.
Kunyanya kana maitiro mazhinji achifanirwa kumisikidzwa, yakagadziriswa kugadzirira iyo inonzi master musanganiswa panzvimbo yekupopa imwe neimwe reagent zvakasiyana mumudziyo wega wega. Pre-configured mixes inotengeswa, umo chete sampuli-chaiyo zvikamu (primer) uye mvura zvinowedzerwa. Neimwe nzira, iyo master musanganiswa inogona kugadzirwa wega. Mune ese ari maviri akasiyana, musanganiswa unogoverwa kumudziyo wega wega wePCR usina template uye sampu yeDNA yega inowedzerwa zvakasiyana kumagumo.
Kushandisa master musanganiswa kune akati wandei mabhenefiti: Chekutanga, nhamba yematanho epipepi imwechete yakaderedzwa. Nenzira iyi, zvose zviri zviviri njodzi yekukanganisa kwevashandisi panguva yepipetting uye njodzi yekusvibiswa inoderedzwa uye, hongu, nguva inochengetwa. Muchidimbu, kurongeka kwepipetting zvakare kwakakwira, sezvo mavhoriyamu akakura akaiswa dosed. Izvi zviri nyore kunzwisisa kana uchitarisa tekinoroji data yepipette: Iyo idiki iyo dosed vhoriyamu, iyo yakakwirira kutsauka kunogona kuve. Ichokwadi chokuti zvose zvakagadzirirwa zvinobva mumudziyo mumwe chete zvine maitiro akanaka pane homogeneity (kana zvakanyatsosanganiswa). Izvi zvakare zvinovandudza kuberekana kwezviedzo.
Paunenge uchigadzira tenzi musanganiswa, inokwana 10% yekuwedzera vhoriyamu inofanirwa kuwedzerwa (semuenzaniso kana gumi gadziriro ichidikanwa, verenga pahwaro hwe11), kuitira kuti kunyange mudziyo wekupedzisira uzadze nemazvo. Nenzira iyi, (zvishoma) zvikanganiso zvepombi, uye mhedzisiro yekurasikirwa kwemuenzaniso apo dosing detergent-ine mhinduro inogona kubhadharwa. Detergents arimo mune enzyme mhinduro senge mapolymerases uye master musanganiswa, zvichikonzera kuumbwa kwefuro uye zvisaririra mukati memukati meyakajairika.pipette mazano.
Zvichienderana nekushandiswa uye rudzi rwemvura inopihwa, iyo chaiyo yepipeting tekinoroji (1) inofanirwa kusarudzwa uye michina yakakodzera yakasarudzwa. Kune zvigadziriso zvine ma detergents, yakananga displacement system kana iyo inonzi "low retention" pipette mazano seimwe nzira yemhepo-cushion pipettes inokurudzirwa. Mhedzisiro yeACE PIPETTE zanoyakavakirwa pane kunyanya hydrophobic pamusoro. Zvinwiwa zvine ma detergents hazvisiye firimu rakasara mukati nekunze, kuitira kuti kurasikirwa kwekugadzirisa kunogona kuderedzwa.
Kunze kweiyo chaiyo dosing yezvikamu zvese, zvakakoshawo kuti hapana kusvibiswa kwegadziriro kunoitika. Hazvina kukwana kushandisa zvinoshandiswa zvekuchena kwepamusoro, nokuti nzira yepipeting mumhepo cushion pipette inogona kubudisa aerosols inoramba iri mupipette. Iyo DNA inogona kunge iri muaerosol inogona kutamiswa kubva kune imwe sampu kuenda kune inotevera mune inotevera pombi nhanho uye nokudaro inotungamira mukusvibiswa. Iyo yakananga masisitimu ekutamisa ataurwa pamusoro anogona zvakare kuderedza njodzi iyi. Kune air-cushion pipettes zvine musoro kushandisa sefa matipi kuchengetedza pipette koni nekuchengetedza splashes, aerosols, uye biomolecules.
Nguva yekutumira: Zvita-06-2022