Mune genetic kutsvagurudza uye mushonga, polymerase keteni reaction (PCR) ndeyeiwo anoshandiswa kushandiswa kwehunyanzvi hwekukwirisa Sampulies ekuedza kwakasiyana siyana. Maitiro aya anovimba zvakanyanya pane pccr anokanganiswa izvo zvakakosha pakuedza kwakabudirira. Munyaya ino, isu tinokurukura nezvakakosha zvinokonzeresa prcr miedzo: PRR mahwendefa, PCR ma-pc machubheni, aishuvira membraes, uye matipi ePipiette.
PCR ndiro:
PCR mahwendefa ndeimwe yeanonyanya kukosha inokanganisika mune chero pccr idzo. Izvo zvakagadzirirwa kukurumidza kupisa cycling uye kupa yunifomu yekushambadzira mukati meiyo yakavezwa yekureruka kwekubata. Mahwendefa anowanikwa mune dzakasiyana-siyana gadziriso kusanganisira 96-zvakanaka, 384-zvakanaka, uye 1536-zvakanaka.
PRR mahwendefa akagadzirwa nepurasitiki, ayo anovaita akavimbika uye nyore kubata. Uye zvakare, mamwe mapiritsi ePRR anounganidzirwa zvakanyanya kundivhiringidza kusungwa kweDNA mamaira uye kudzivirira kusvibiswa. Iko kushandiswa kwePRC mahwendefa kwakakosha kudzikisa matanho mazhinji-akanyanya kuitwa mu microcentrifugger kana prcr michina.
Pcr Tube:
PCR ma tubes madiki madiki, kazhinji akagadzirwa ne polypropylene, yaishandiswa kubata iyo prcr yekuita musanganiswa panguva yekuwedzera. Ivo vanouya mune dzakasiyana-siyana mavara, asi zvakanyanya kujeka uye translucent. Bvisa PCR Tubes kazhinji inoshandiswa kana vashandisi vanga vachida kuona ampliuro soice nekuti ivo vanoonekera.
Aya machubhu akagadzirirwa kutsungirira kupisa kwakanyanya uye kudzvanywa kunowanikwa muPRR michina, kuzviita kwakanakira plcr miedzo. Kuwedzera kune kukwidziridzwa, PCR ma tubes inogona kushandiswa kune mamwe mashandisirwo akadai seDNA kuti atsvake uye kucheneswa uye kuongororwa kwechidimbu.
Sungai Firimu:
Seal firimu ndeyepurasitiki yepurasitiki yakasungirirwa kumusoro kweiyo PCR ndiro kana thubhu kudzivirira kubuda uye kusvibiswa kwemusanganiswa wePCR. Mafirimu emvura akakosha zvakanyanya muPRR kuedza, sekusarudzika kwemisangano kana chero kusvibiswa kwezvakatipoteredza mundiro inogona kukanganisa basa uye kushanda kwekuedza.
Yakagadzirwa nePolyethene kana Polypropylene, zvichienderana nekushandisa, mafirimu epurasitiki aya akanyanya kupisa anodzivirira uye anomiradzwa. Mamwe mafirimu anotanga kutemwa chaiyo prcr elates uye machubhu, nepo vamwe vachiuya mumatanda uye vanogona kushandiswa nemhando dzakasiyana dzePCR mahwendefa kana machubhu.
Pipiette Matipi:
Pipette matipi akakosha anokoshesa zvekushandisa plcr miedzo, sezvo ivo vanoshandiswa kuendesa zvidiki zvidiki zvemvura, senge samples kana reagents. Ivo vanowanzogadzirwa nePolyethyle uye vanogona kubata mimhanzi kubva ku0.1 μl kusvika 10 ml. Pipiette matipi aratidzwe uye yakagadzirirwa kushandiswa kamwe chete.
Kune marudzi maviri ePIETT Tiips - Yakasviba uye isina-yakasviba. Mazano efaira akakodzera kudzivirira chero aerosol kana kudzikisira kusvibiswa kubva kuitika, nepo isiri-firita matipi anoshandiswa pakuedza kwePRR iyo isorganic soles kana caustic mhinduro.
Mune pfupiso, prcr mahwendefa, PCR maleti, kuisimbisa membranes, uye matipi ePipette ingori zvimwe zvezvakakosha zvinodikanwa zvinodikanwa perliment. Nekuita kuti iwe uve nezvinhu zvese zvinodiwa zviyero, unogona kuita nani plcr kuedza zvakanaka uye nekukwana kwaunoda. Naizvozvo, gara uine shuwa kuti iwe unazvo wakakwana pane izvi zvinokatyamadza zviri nyore kuwanikwa kune chero prcr kuedza.
At Suzhou Ace Biemiemical, isu takatsaurirwa kukupa iwe nemhando yepamusoro yemhando yeLab inopa kune zvese zvaunoda zvesainzi. Mhando yedu yePipiette Matipi, PCR mahwendefa, PCR Tubes, uyekusimbisa firimuvakanyorerwa zvakagadzirwa uye vakacheneswa kuti vave nechokwadi chekunyatsojeka uye chokwadi mune zvese zvaunoedza. Tipei pipette matipi anoenderana nemhando dzese dzakakura dze pombi uye huya mune dzakasiyana siyana dzehukuru kuti dzienderane nezvako zvaunoda chaizvo. Mahwendefa edu ePCR ePRR uye machubhu anogadzirwa kubva kune yakakwirira-mhando zvinhu uye zvakagadzirirwa kutsungirira akawanda maTermal matinji paunenge uchichengeta kutendeka kwemuenzaniso. Firimu redu rekuisa chisimbiso rinopa chisimbiso chakasimba kudzivirira kubuda uye kusvibiswa kubva kunze zvinhu. Isu tinonzwisisa kukosha kwekuvimbika uye kwakashanda lab Chikwata chedu chevanyanzvi chinogara chiripo kuti chikubatsire iwe chero mibvunzo kana kunetsekana zvaungave uine.
Kutumira Nguva: May-08-2023