Mukutsvagisa majini uye mushonga, polymerase chain reaction (PCR) inzira inowanzoshandiswa mukukudza DNA samples dzekuyedza kwakasiyana. Maitiro aya anonyanya kutsamira paPCR zvinodyiwa izvo zvakakosha pakuedza kwakabudirira. Muchinyorwa chino, tinokurukura zvakakosha zvinobatika kune yakazara PCR kuyedza: PCR mahwendefa, PCR machubhu, yekuisa chisimbiso membranes, uye pipette matipi.
PCR ndiro:
PCR mahwendefa ndechimwe chezvinhu zvakakosha zvinodyiwa mune chero PCR kuyedza. Iwo akagadzirirwa kukurumidza tembiricha bhasikoro uye anopa yunifomu kupisa kupisa mukati mebore kuitira nyore kubata. Iwo mahwendefa anowanikwa mune akasiyana masisitimu anosanganisira 96-tsime, 384-tsime, uye 1536-tsime.
PCR mahwendefa akagadzirwa epurasitiki, izvo zvinoita kuti zvive zvakavimbika uye nyore kubata. Pamusoro pezvo, mamwe maPCR mahwendefa akanyatso kuvharidzirwa kuti avhare kusungwa kwemamorekuru eDNA uye kudzivirira kusvibiswa. Kushandiswa kwePCR mahwendefa kwakakosha kudzikisa nhanho dzebasa dzakamboitwa mumamicrocentrifuges kana PCR michina.
PCR chubhu:
PCR machubhu machubhu madiki, anowanzo gadzirwa nepolypropylene, anoshandiswa kubata PCR reaction musanganiswa panguva yekukudza. Zvinouya mumarudzi akasiyana-siyana, asi zvinowanzoitika zvakajeka uye zvinopenya. Bvisa PCR machubhu anowanzo shandiswa kana vashandisi vachida kuona amplified DNA nekuti iri pachena.
Aya machubhu akagadzirirwa kushingirira tembiricha yakakwira uye madhindindi anowanikwa mumashini ePCR, achiaita akanakira kuyedza PCR. Pamusoro pekuwedzera, PCR machubhu anogona kushandiswa kune mamwe maapplication akadai seDNA kutevedzana uye kucheneswa uye chidimbu chekuongorora.
Kuvhara firimu:
Seal firimu ifirimu rinonamira repurasitiki rakanamirwa pamusoro pePCR ndiro kana chubhu kudzivirira kubuda uye kusvibiswa kwemusanganiswa wekuita panguva yePCR. Kuisa chisimbiso mafirimu kwakakosha zvakanyanya muPCR kuyedza, sezvo yakafumurwa maitiro musanganiswa kana chero kusvibiswa kwezvakatipoteredza mundiro kunogona kukanganisa kutendeseka uye kushanda kwekuyedza.
Yakagadzirwa nepolyethylene kana polypropylene, zvichienderana nekushandiswa, aya mafirimu epurasitiki anodzivirira kupisa zvakanyanya uye autoclavable. Mamwe mafirimu akafanochekwa kune chaiwo PCR mahwendefa uye machubhu, nepo mamwe achiuya mumapumburu uye anogona kushandiswa nemhando dzakasiyana dzePCR mahwendefa kana machubhu.
Pipette mazano:
Pipette matipi akakosha ekushandisa kune PCR kuyedza, sezvo anoshandiswa kuendesa zvidiki zvemvura, senge sampuli kana reagents. Anowanzo gadzirwa nepolyethylene uye anogona kubata mavhoriyamu emvura kubva pa0.1 µL kusvika pa10 mL. Pipette matipi anoraswa uye anoitirwa kushandiswa kumwe chete.
Kune marudzi maviri epipette mazano - akacheneswa uye asina-sefa. Mazano ekusefa akakodzera kudzivirira chero kusvibiswa kweaerosol kana kudonhedza kubva kuitika, nepo asiri-sefa matipi anoshandiswa paPCR kuyedza uchishandisa inorganic solvents kana caustic mhinduro.
Muchidimbu, mahwendefa ePCR, machubhu ePCR, membrane yekuisa chisimbiso, uye matipi epipetti zvinongori zvimwe zvezvinhu zvinobatika zvinodikanwa pakuyedza PCR yakazara. Nekuona kuti une zvese zvinodiwa zvekushandisa, unogona kuita zvirinani zviedzo zvePCR nemazvo uye nekurongeka kwaunoda. Naizvozvo, gara uine shuwa kuti une zvakakwana zvezvinhu izvi zvinoshandiswa zviri nyore kune chero PCR kuyedza.
At Suzhou Ace Biomedical, isu takazvipira kukupa iwe yemhando yepamusoro yelab rekushandisa kune zvese zvaunoda zvesainzi. Our range ofpipette mazano, PCR mahwendefa, PCR machubhu,uyefirimu rekusimbisaakagadzirwa zvine hungwaru uye akagadzirwa kuti ave nechokwadi chechokwadi uye chokwadi mune zvese zvaunoedza. Mazano edu epipette anowirirana nemhando dzose huru dzepipette uye anouya mumhando dzakasiyana-siyana kuti aenderane nezvido zvako. Yedu PCR mahwendefa uye machubhu anogadzirwa kubva emhando yepamusoro zvinhu uye akagadzirirwa kutsungirira akawanda ekupisa kutenderera uku uchichengetedza kuvimbika kwemuenzaniso. Yedu yekuisa chisimbiso firimu inopa chisimbiso chakasimba kudzivirira evaporation uye kusvibiswa kubva kunze kwezvinhu. Isu tinonzwisisa kukosha kweakavimbika uye anoshanda lab ekushandisa, ndosaka tichiyedza kukupa iwe zvigadzirwa zvemhando yepamusoro uye masevhisi anogoneka. Chikwata chedu chenyanzvi chinogara chiripo kuti chikubatsire iwe chero mibvunzo kana zvinokunetsa zvaungave nazvo.
Nguva yekutumira: Chivabvu-08-2023