Unocheka herepipette tippanguva yekuputira glycerol? Ndakaita panguva yePhD yangu, asi ndaifanira kudzidza kuti izvi zvinowedzera kusarongeka uye kusarongeka kwepombi yangu. Uye kutaura chokwadi pandakacheka muromo, ndingadai ndakadururawo zvakananga glycerol kubva mubhodhoro kupinda muchubhu. Saka ndakashandura maitiro angu kuti ndivandudze mhedzisiro yepipetting uye ndiwane yakavimbika uye inobereka mhedzisiro kana ndichishanda ne viscous zvinwiwa.
Chikamu chemvura chinoda kutariswa zvakanyanya kana pipetting iri viscous zvinwiwa. Izvi zvinowanzo shandiswa murabhoritari, ingave mune yakachena fomu kana sebuffer zvikamu. Vamiriri vakakurumbira vezvinwiwa zveviscous mumarabhoritari ekutsvagisa glycerol, Triton X-100 uye Tween® 20. Asi zvakare, marabhoritari anoita kudzora kwemhando yezvokudya, zvizoro, mishonga uye zvimwe zvigadzirwa zvevatengi zvinotarisana nemhinduro dze viscous zuva nezuva.
Viscosity inogona kutaurwa seine simba, kana kinematic viscosity. Muchinyorwa chino ini ndinotarisisa pane dynamic viscosity yezvinwiwa sezvo ichitsanangura mafambiro emvura. Dhigirii ye viscosity inotsanangurwa mu millipascal pasekondi (mPa * s). Asi masampuli emvura akatenderedza 200 mPa*s akadai se85 % glycerol anogona achiri kutamiswa uchishandisa yemhando yepamusoro air cushion pipette. Paunenge uchiisa yakakosha tekinoroji, reverse pipetting, aspiration yemhepo mabhubhu kana zvisaririra mumuromo zvinodzikiswa zvakanyanya uye zvinotungamira kune yakanyanya kurongeka pombi mhedzisiro. Asi zvakadaro, haisi iyo yakanakisa yatingaite kuti tivandudze pipetting ye viscous liquids (ona fig. 1).
Kana viscosity ichiwedzera, matambudziko anowedzera. Epakati viscous mhinduro dzinosvika 1,000 mPa*s dzakanyanya kunetsa kutamiswa uchishandisa classic air-cushion pipettes. Nekuda kwekukwesha kwemukati kwemamorekuru, viscous zvinwiwa zvine maitiro anononoka kuyerera uye pipetting inofanirwa kuitwa zvishoma nezvishoma uye nekungwarira. Reverse Pipetting tekinoroji kazhinji haina kukwana kuchinjisa mvura uye vanhu vazhinji vanoyera sampuli dzavo. Iyi nzira zvakare inoreva kutora density yemvura mukufunga pamwe nemamiriro emurabhoritari senge mwando uye tembiricha kuti uverenge nemazvo huremu hunodiwa hwemvura. Naizvozvo, mamwe maturusi epombi, anonzi maturusi ekutamisa zvakanaka, anokurudzirwa. Aya ane muromo une piston yakabatanidzwa, sejekiseni. Nekudaro, mvura inogona kuve nyore kushuvira uye kugovaniswa nepo chaiyo yekufambisa mvura ichipihwa. Imwe nzira yakakosha haidiwi.
Zvakangodaro, zvakare maturusi ekutamisa zvakanaka anosvika pamuganho ane viscous mhinduro dzakadai sehuchi hwemvura, ganda rekirimu kana mamwe mafuta emuchina. Izvi zvinwiwa zvinonyanya kudiwa zvinoda chimwe chishandiso chakakosha icho zvakare chinoshandisa iyo yakanaka yekubvisa musimboti asi zvakare ine yakagadziridzwa dhizaini yekutarisana nemhinduro dzakanyanya viscous. Ichi chakakosha chishandiso chakafananidzwa nearipo matipi ekutamisa zvakanaka kuti uwane chikumbaridzo apo zvakakosha kuti uchinje kubva kune yakajairwa yekugovera tipi kuenda kune yakakosha zano kune yakanyanya viscous mhinduro. Zvakaratidzwa kuti kurongeka kunowedzerwa uye masimba anodiwa pakushuvira uye kugovera anoderedzwa kana uchishandisa zano rakakosha kune zvakanyanya viscous zvinwiwa. Kuti uwane rumwe ruzivo rwakadzama uye mienzaniso yemvura, ndapota dhawunirodha Applicaton Note 376 pane yakagadziridzwa mashandiro ezvinwiwa zvakanyanya viscous.
Nguva yekutumira: Jan-23-2023