Iyo revolutionary polymerase chain reaction (PCR) maitiro akaita mupiro wakakura mukufambira mberi muruzivo rwevanhu munzvimbo dzakawanda dzekutsvaga, kuongororwa uye forensics. Misimboti yePCR yakajairwa inosanganisira kukwidziridzwa kweDNA kutevedzana kwekufarira mumuenzaniso, uye mushure mekupedzwa kwekuita, kuvapo kana kusavapo kweiyo DNA kutevedzana kunotemwa pakupera kwekuongorora. Munguva yeCovid-19 denda, chaiyo-nguva PCR inoyera kuunganidzwa kwezvigadzirwa zvekusimudzira sezvo maitiro anofambira mberi, achipa huwandu mushure mekutenderera kwega kwega, yave nzira yegoridhe-yakajairwa yekuyedza varwere kuongororwa kweSARS-COV-2.
Real-time PCR, inozivikanwawo sehuwandu PCR (qPCR), inoshandisa akasiyana akasiyana fluorescent chemistries inoenderana nePCR chigadzirwa kusungwa kune fluorescence intensity. Mushure mePCR yega yega kutenderera, fluorescence inoyerwa uye kusimba kweiyo fluorescence chiratidzo kunoratidza huwandu hweDNA amplicon mumuenzaniso panguva iyoyo chaiyo. Izvi zvinogadzira qPCR curve, umo inotsanangurwa chiratidzo chekusimba kunofanirwa kupfuudzwa kusvika pave nechigadzirwa chakakwana chekuti fluorescence ionekwe pamusoro pekumashure. Iyo curve inoshandiswa kuona huwandu hwechinangwa cheDNA.
Nekufamba kwenguva, marabhoritari akashandisa kushandiswa kwemaplate-matsime akawanda kugadzira masampuli akawanda panguva imwe chete, zvichibvumira kubuda kwepamusoro. Nekudaro, iwo masampuli anofanirwa kuchengetedzwa kubva pakusvibiswa uye kubuda kuti ive nemhando yepamusoro yemhedzisiro. Iyo PCR tekinoroji inotarisisa zvakanyanya kusvibiswa nekunze kweDNA, saka zvakakosha kuchengetedza nharaunda yakachena. Kunyanya kujeka kwemaziso uye kupindira kushoma kwakakosha kuti uve nechokwadi chekuverenga chaiko kwechiratidzo chefluorescent. PCR plate seals iripo kuti iite basa iri uye kune marudzi akasiyana ezvisimbiso anowanikwa kune akasiyana sampuli, maitiro ekuedza uye zvido zvemunhu. Kuenzaniswa nedzimwe nzira dzekuisa chisimbiso, kushandiswa kwekunamira ndiro yekuisa chisimbiso iri nyore uye inodhura-inoshanda.
Kuvhara mafirimu kubvaSuzhou Ace Biomedicalvane high optical kujekesa nekusatora, kusina-fluorescing yekurapa giredhi adhesive, inokodzera chaiyo-nguva PCR application. Aya maficha akakosha kuve nechokwadi chekuti mafirimu ekuisa chisimbiso haakonzerese kukanganisa kune zvakawanikwa.
Iwo ekuisa chisimbiso mafirimu zvakare aka certified DNase, RNase uye nucleic acid yemahara saka vashandisi vanogona kuve nechokwadi chekuti hapana kusvibiswa kwemasampuli uye mhedzisiro ndeyechokwadi.
Ndeapi Mabhenefiti EAdhesive Seals?
Adhesive seals inokurumidza uye iri nyore kushandisa ine yakananga application pamusoro pemapureti mune manual workflows kuchengetedza zviri mukati mahwendefa kwenguva pfupi. Uye iyo inopindirana yepamusoro-soro kujeka kwemaziso kunoita kuti iwedzere kudhindwa, yakavimbika uye yakarurama DNA amplification zviyero.
Inamira inert, yakasimba, isingaite tembiricha inovimbisa kuvharika kwakavimbika patsime rega rega. Iwo zvakare ane maviri-ekupedzisira ma tabo anobatsira mukuisa chisimbiso firimu uye anogona kubviswa kudzivirira kusimudza uye yakakwirira evaporation mitengo.
Kuvhara mafirimu kunoderedza kubuda kwemhepo, kuderedza kusvibiswa kwemuchinjiko uye kudzivirira kupera - izvo zvakakosha zvakanyanya kana uchibata nemasampuli ane hutachiona uye bhakitiriya mamorekuru achiisa njodzi kumunhu.
Mhando yakawanda yezvimwe zvisimbiso zveplate zvinowanikwa kubvaSuzhou Ace Biomedicaline chaiyo zvivakwa zvakagadzirirwa maapplication akadai seyakajairwa PCR, yenguva pfupi uye kwenguva refu yekuchengetedza.
Nguva yekutumira: Sep-15-2022