Cryovilszvinowanzoshandiswa kuchengetedzwa kweCrogenic yemitsetse yemitsetse uye zvimwe zvinhu zvakashata zvezvinhu, mumahara akazadzwa neyechivhi nitrogen.

Kune matanho akati wandei anobatanidzwa mukuchengetedza kwakabudirira kwemasero mumvura nitrogen. Ipo nheyo yekutanga ndeyekutanga kusununguka, maitiro chaiwo akasarudzika zvinoenderana nemhando yemhando uye cryoprotectant yakashandiswa. Pane akati wandei ekuchengetedza kufunga uye maitiro akanakisa kuti afunge kana kuchengetedza masero pakupisa kwakadaro kupisa.

Iyi posvo inovavarira kupa pamusoro pekunyatsoongorora kuti ndedzipi cryovils inochengeterwa mumvura nitrogen.

Chii chakatsinhana

Cryovils idiki, dzakarongedzwa viums dzakagadzirirwa kuchengetedza samples dzemvura pakudziya kwakanyanya. Ivo vanoona kuti masero akachengetedzwa muClypoteant haakezve kusangana neyenzi nitrogen yeNotrogens

Makomels anowanzo kuwanikwa mumhando yemavhoriyamu uye magadzirirwo - iwo anogona kuve ari mukati kana kunze kwekunze neakapetwa kana kutenderera mabhodhoro. Yakasvibiswa uye isiri-isina kugadzirwa mafomu anowanikwa zvakare.

AnoshandisaCyrovialskuchengeta masero mumvura nitrogen

Mhando dzeNHS uye dzakazvimirira madhiri, pamwe nekutsvaga masisitiri kungwarira mubhangi reropa, Epithelial cell biology, immunology uye stem biology biology shandisa Cryovillals kune Cryology Cryology masero.

Masero akachengetwa nenzira iyi anosanganisira B uye t masero, cho chogentipoetic masero, maseru emberi, macrophally maseru, Monenititor, myoloma, myoloma, myeloma, nk maseru uye pluripotent maseru maseru.

Kuongorora kwe Maitiro ekuchengetera Cryovials mumvura nitrogen

Cryopreservationerting iction iyo inochengetedza masero uye zvimwe zvekugadzira biology nekuvatonhorera kune yakaderera tembiricha. Masero anogona kuchengetwa mumvura nitrogen kwemakore pasina kurasikirwa kwesero kubhadharisa. Ichi chirevo chezvemaitiro akashandiswa.

Kugadzirira cell

Nzira chaiyo yekugadzirira samples ichasiyana zvichienderana nerudzi rwemhando, asi zvakawandisa, masero anounganidzwa uye akaunganidzwa kuti ave neclet-yakapfuma pellet. Iyi pellet inobva yasimbiswa mune supernatant yakasanganiswa ne cryoprotect kana nzvimbo yekrineservreservation.

Cryopreservation Spetium

Iyi svikiro inoshandirwa kuchengetedza masero munzvimbo dzakadzika-tembiricha dzavanozoiswa pasi nekunyudza kuumbwa kweInra uye extressury makristasi uye nokudaro cell kufa. Basa ravo nderokupa nharaunda yakachengeteka, inodzivirira yemasero uye matishu panguva yekusununguka, kuchengetedza, uye maitiro uye thawing maitiro.

Svikiro rakadai seyakagadzirwa nechando plasma.

Iyo yekudzoswa-inodonhedza pellet yakadzvanywa mune polypropylene cryovils yakadai seSuzhou Ace Biomonyical Kambani Cneyogenic Yekuchengetedza Vials.

Izvo zvakakosha kuti usanyanya kuunganidzira iyo tsananguro sezvo izvi zvichawedzera njodzi yekupaza uye kusunungurwa kwazvino kuitika (1).

Inodzorwa freeze chiyero

Kazhinji, chiyero chinononoka chekudzora chinoshandirwa chinoitwa kune yakabudirira kudzikiswa kwemasero.

Mushure mekunge samples dzakatangira muCryogenic vials vials, ivo vanoiswa pane yakanyorova ice kana mune 4 ℃ firiji uye maitiro ekusununguka anotangwa mukati memaminitsi mashanu. Segirairo general, masero anotonhorera pachiyero che -1 kusvika -3 paminiti (2). Izvi zvinowanikwa uchishandisa zvirongwa zvekutonhora kana nekuisa vials mune yakavezwa bhokisi yakaiswa mu -70 ° C kusvika -90 ° C yakatsoropodzwa freezer.

Chinjana kune mvura nitrogen

Iyo yechando cryogenic vials inobva yaendeswa kuTank yeNitrogen yeNitrogen yenguva isingagumi yakapa tembiricha isina kushoma pane -135 ℃ inochengetwa.

Aya ekupedzisira-akaderera tembiricha anogona kuwanikwa nekunyudzwa mumvura kana muputeshi photrogen.

Liquid kana vapor chikamu?

Kuchengetera mumvura chikamu nitrogen kunozivikanwa kuchengetedza tembiricha inotonhora nemhedzisiro zvachose, asi kazhinji haina kukurudzirwa nekuda kwezvikonzero zvinotevera:

• Iko kudikanwa kwemavhoriyamu makuru (kudzika) kwemvura nitrogen iyo iri njodzi inogona kuitika. Inopisa kana asphyxiation nekuda kweiyi njodzi chaiyo.
• Zvinyorwa zvakanyorwa zvekuyambuka nevamiririri vane hutachiona senge aspergillus, hep b uye viration vakapararira kuburikidza neyedya nitrogen pakati (2,3)
• Mukana wekuti nitrogener yemvura kuti ubude mumizambiringa panguva yekunyudzwa. Kana yabviswa kubva pakuchengetedza uye kudziyirwa kudziya tembiricha, iyo nitrogen nekukurumidza inowedzera. Nekuda kweizvozvo, iyo vial inogona kupwanya kana yabviswa kubva kumvura nitrogen yekuchengetedza, ichigadzira njodzi kubva pamarara emukati uye kuratidzwa kune zvirimo (1, 4).

Nezvikonzero izvi, Ultra-lol-low tembiricha inochengeterwa zvakanyanya muVapor phase nitrogen. Kana samples dzinofanira kuchengetwa mumvura chikamu, nyanzvi yeCryoflex tubing inofanira kushandiswa.

Mufambiro wepasi pane iyo vapoter chikamu ndechekuti iyo tembiricha yekupisa inogona kuitika mukudzikama mukudziya kwekushisa pakati -135 ℃ uye -190 ℃. Izvi zvinoda kutarisisa uye kushingairira kutarisa kwemvura yeiyo nitrogen mazinga uye tembiricha misiyano (5).

Vazhinji vagadziri vanokurudzira kuti Cryovils akakodzera kuchengetwa pasi kusvika -135 ℃ kana yekushandisa mumuputi phosha chete.

Tywing Cryop Ry Cell

Maitiro ekutambisa anoshungurudza nekuda kwetsika yechando, uye kubata kwakakodzera uye maitiro anodikanwa kuti ave nechokwadi chekukwirisa, kupora, uye kushanda kwemasero. Iyo chaiyo tywing protocol inotenderana nemhando dzechitokisi. Nekudaro, nekukurumidza thawing inoonekwa seyakaenzana:

• Kuderedza chero kukanganisa pane cellular kupora
• Batsira kuderedza nguva yekufumura kune iyo solutes inounza muzvikamu zvekusununguka
• Kuderedza chero kukuvara neIce RECRYSTALISINATION

Mvura yekugezera, mabhati ebhatano, kana zviridzwa zvine midziyo zvinowanzoshandiswa zvinowanzo shandiswa kusarudzika samples.

Kazhinji kakawanda cell line inguva yemaminitsi 1, nekukwira zvinyoro-nyoro mumvura yekugeza kwemvura kusvikira pano rive diki diki isati yashambidzwa kukura kwepakati.

Kune mamwe masero akadai seammalian embryos, kudziya zvishoma kunononoka kwakakosha pakupona kwavo.

Iwo masero ave agadzirira tsika yemasero, masero ekuzviparadzanisa, kana mune nyaya yeHematipoetic Stem masero - Vablogity zvidzidzo kuti vimbiso kutendeka kweanopa dhizaini remahara pamberi pesimba remyweblative.

Zvakajairika kuita kuti utore madiki madiki emuenzaniso wakasvibiswa wakashandiswa kuita iyo Cell kuverenga kuti uone kutarisisa masero ekurongedza mutsika mutsika. Iwe unogona ipapo ongorora mhedzisiro yesero yekuzvisarudzira maitiro uye tarisa cell vibility.

Zviitiko zvakanakisa zvekuchengetedza kwetsvanyiriri

Iyo yakabudirira cryopreservation ye samples yakachengetwa muCryovils inoenderana nezvakawanda zvinhu mune protocol inosanganisira kuchengetwa kwakakodzera uye rekodhi kuchengeta.

• Kupatsanura masero pakati penzvimbo dzekuchengetedza- Kana mavhoriyamu achibvumira, kupatsanura masero pakati pevarume uye kuchengeta munzvimbo dzakaparadzana kuti dzideredze njodzi yemitambo yemuviri nekuda kwemidziyo kukundikana.
• Dzivirira kuyambuka-kusvibiswa- Sarudza imwe-shandisa serile cryogenic vials kana autoclave usati wazotevera kushandiswa
• Shandisa zvakakodzera zvakaringana vials yemasero ako- viums inouya mune dzakasiyana siyana mavhoriyamu pakati 1 ne5MS. Ngwarira kuvanzika mapoka kuti uderedze njodzi yekupaza.
• Sarudza yemukati kana kunze kwekusheedzera cryogenic vials- Inyika Yakarongedzwa Varume inokurudzirwa nemamwe mayunivhesiti ezvekuchengetedza matanho - ivo vanokwanisa zvakare kudzivirira kusvibiswa panguva yekuzadza kana kuchengetwa mumvura nitrogen.
• Dzivirira Kuzvidza- Shandisa Bi-jekiseni zvisimbiso zvakaumbwa muzvikamu-cap-cap kana o-mhete kudzivirira kubhururuka uye kusvibiswa.
• Shandisa 2D Barcode uye Label Vals- Kuti uve nechokwadi chekutengeserana, vials ine nzvimbo dzakakura dzekunyora dzinogonesa vial kuti uve wakakwana wakanyorwa. 2D Barcode inogona kubatsira nekuchengetedza manejimendi uye rekodhi kuchengeta. Ruvara rwemakadhi macaps anobatsira pakuzivikanwa nyore.
• Kuchengetedza zvakakwana kuchengetedza-Kuona masero haana kurasika, midziyo yekuchengetedza inofanirwa kugara ichitarisa tembiricha uye mvura nitrogen mazinga. Maaramu anofanirwa kuve akakodzera kuvanzika vashandisi vekukanganisa.

Kuchengetedza Kuchengetedza

Liquid nitrogen yave kuzivikanwa mukutsvagisa kwemazuva ano asi inotakura njodzi yekukuvara kwakanyanya kana ikashandiswa zvisizvo.

Zvakakodzera zvekudzivirira pachako Pfeka

• Cryogenic Gloves
• Laboratory Coat
• IMPACT KUSHANDISA IZVO ZVAKAITWA SHIGE SHICE inovharawo mutsipa
• Yakavharwa-Toe shangu
• SplashProof Plastiki Apron

Firtrogen mafiriji anofanirwa kuiswa munzvimbo dzakanyatsovhurwa kuti uderedze njodzi yeasphyxiation - yakapukunyuka nitrogen vaporiisa oxygen. Zvizoro zvakakura zvekuchengetedza zvinofanirwa kuve ne lovegen yakaderera alarm sylems system.

Kushanda vaviri vaviri kana kubata mvura nitrogen kwakanakira uye kushandiswa kwayo kunze kweyakajairika maawa ekushanda anofanirwa kurambidzwa.

Cryovils kutsigira yako yebasa

Suzhou Ace BiemiMical Company inopa kusarudzika kwakasiyana kwezvigadzirwa zvinosangana nezvinodiwa zvako zvezvakareruka zvinodiwa nemhando dzakasiyana dzemasero. Iyo portfolio inosanganisira huwandu hwe tubesas zvakanaka seyakaenzana yerudzi rwekrisimasi.

Yedu Cryovills ndeiyi:

• Lab screw cap 0.5ml 1.5ml 2.0ml clyovial vialsenic vials concal pasi zlotube neasetket

  ● 0.5ML, 1.5ML, 2.0ml kutaurwa, ine siketi kana isina siketi
  ● Conical kana kuzvimira magadzirirwo, serile kana isiri-isina kujairika iripo
  ● Screw cap tubes yakagadzirwa neyekurapa giredhi polypropylene
  ● PP Cryotube vials inogona kudzokororazve chando uye tilwed
  ● Iyo yekunze cap dhizaini inogona kuderedza iyo inosvibiswa mukana panguva yekuenzanisira kurapwa.
  ● Screw cap Cryogenic tubes Universal screw screws yekushandisa
  ● Tubs zvinokwana kazhinji rombo revanhu
  ● Cryogenic tube o-ring tubes fit standard 1-inch and 2-inch, 48well, 81well，96well and 100well freezer boxes
  ● Autlavable kusvika pa121 ° C uye inoshamisa kune -86 ° C

  Chikamu kwete	Zvinhu	Vhoriyamu	CapRuvara	PCS /Bhegi	Mabhegi / kesi
  Act05-bl-n	PP	0.5ml	Dema, yero, yebhuruu, tsvuku, yepepuru, chena	500	10
  Act15-bl-n	PP	1.5ml	Dema, yero, yebhuruu, tsvuku, yepepuru, chena	500	10
  Act15-bl-nw	PP	1.5ml	Dema, yero, yebhuruu, tsvuku, yepepuru, chena	500	10
  Act20-bl-n	PP	2.0ml	Dema, yero, yebhuruu, tsvuku, yepepuru, chena	500	10