Cryovialsanowanzo shandiswa kuchengetera cryogenic yemasero mitsara uye zvimwe zvakakosha zvipenyu zvipenyu, mumadewa akazadzwa nemvura nitrogen.

Pane zvinhanho zvakati wandei zvinosanganisirwa mukubudirira kwekuchengetedza maseru mu liquid nitrogen. Nepo musimboti wekutanga uri kutonhora kunononoka, iyo chaiyo nzira inoshandiswa inoenderana nerudzi rwesero uye cryoprotectant inoshandiswa. Pane zvakati wandei zvekuchengetedza uye maitiro akanakisa ekufunga nezvazvo kana uchichengeta maseru pane yakaderera tembiricha.

Iyi posvo ine chinangwa chekupa tarisiro yekuti ma cryovials anochengetwa sei mumvura nitrogen.

Chii chinonzi Cryovials

Cryovials madiki, akavharirwa mabhodhoro akagadzirirwa kuchengetedza mvura samples pane yakanyanya kuderera tembiricha. Ivo vanove nechokwadi chekuti maseru akachengetedzwa mu cryoprotectant haauye akasangana neiyo nitrogen yemvura, ichideredza njodzi yekutyoka kweserura ichiri kubatsirwa kubva mukutonhora kwakanyanya kwemvura nitrogen.

Mabhodhoro anowanzo kuwanikwa muhuwandu hwemavhoriyamu uye magadzirirwo - anogona kunge ari mukati kana kunze akarukwa neakadzika kana akatenderedzwa pasi. Mafomati asina mbereko uye asina mbereko anowanikwawo.

Ndiani AnoshandisaCyrovialsKuchengeta Masero muLiquid Nitrogen

Huwandu hweNHS nemarabhoritari akazvimirira, pamwe nemasangano ekutsvagisa anoshanda mukubhengi kweropa, epithelial cell biology, immunology uye stem cell biology inoshandisa cryovials kuchengetedza maseru.

Masero akachengetedzwa nenzira iyi anosanganisira B uye T Cells, CHO Cells, Hematopoietic Stem uye Progenitor Cells, Hybridomas, Intestinal Cells, Macrophages, Mesenchymal Stem uye Progenitor Cells, Monocytes, Myeloma, NK Cells uye Pluripotent Stem Cells.

Mhedziso yeMaitiro Ekuchengetedza Cryovials muLiquid Nitrogen

Cryopreservation inzira inochengetedza maseru uye zvimwe zvipenyu zvinovaka nekudzitonhodza kusvika kune yakaderera tembiricha. Masero anogona kuchengetwa munitrogen yemvura kwemakore pasina kurasikirwa kwesero. Ichi chiratidziro chemaitiro anoshandiswa.

Kugadzirira Sero

Iyo chaiyo nzira yekugadzirira sampuli ichasiyana zvichienderana nerudzi rwesero, asi kazhinji, maseru anounganidzwa uye anoiswa mukati kuti agadzire sero-rakapfuma pellet. Iyi pellet inozomiswa zvakare mu supernatant yakasanganiswa ne cryoprotectant kana cryopreservation medium.

Cryopreservation Medium

Iyi svikiro inoshandiswa kuchengetedza maseru munzvimbo dzakadzika-tembiricha dzavanozoiswa pasi padzo nekudzivisa kuumbwa kwe intra uye extracellular crystals nekudaro kufa kwesero. Basa ravo nderekupa yakachengeteka, inodzivirira nharaunda yemasero uye matishu panguva yechando, kuchengetedza, uye kunyungudika maitiro.

Iyo yepakati yakaita senge fresh frozen plasma (FFP), heparinised plasmalyte solution kana serum-isina, mhuka-isina mhinduro inosanganiswa ne cryoprotectants senge dimethyl sulphoxide (DMSO) kana glycerol.

Iyo re-liquified sample pellet inoiswa mu polypropylene cryovials sengeSuzhou Ace Biomedical kambani Cryogenic Storage Vials.

Izvo zvakakosha kuti usazadze iyo cryovials sezvo izvi zvichiwedzera njodzi yekutsemuka uye nekugona kuburitswa kwemukati (1).

Inodzorwa Kumisa Chiyero

Kazhinji, inononoka inodzorwa yechando mwero inoshandiswa kune yakabudirira cryopreservation yemasero.

Mushure mekunge masampula aiswa mumidziyo ye cryogenic, anoiswa pachando chakanyorova kana mufiriji 4 ℃ uye maitiro echando anotanga mukati memaminitsi mashanu. Semutungamiri mukuru, masero anotonhodzwa pamwero we -1 kusvika -3 paminiti (2). Izvi zvinowanikwa uchishandisa chinotonhorera chinorongwa kana nekuisa mabhodhoro mubhokisi rakaiswa insulated rakaiswa mu -70°C kusvika -90°C controlled rate freezer.

Chinja kune Liquid Nitrogen

Iwo akaomeswa nechando cryogenic vials anobva aendeswa kune yemvura nitrogen tangi kwenguva dzisingaperi kana tembiricha isingasviki -135 ℃ ichichengetwa.

Iyi tembiricha yakanyanya-yakaderera inogona kuwanikwa nekunyudzwa mumvura kana mhute chikamu nitrogen.

Liquid kana Vapor Phase?

Kuchengeta muchikamu chemvura nitrogen inozivikanwa kuchengetedza tembiricha inotonhora nekunyatsoenderana, asi kazhinji haikurudzirwe nekuda kwezvikonzero zvinotevera:

• Kudiwa kwemavhoriyamu makuru (kudzika) kwenitrogen yemvura inova njodzi inogona kuitika. Kupisa kana kufemereka nekuda kweizvi injodzi chaiyo.
• Mhosva dzakanyorwa dzekuchinjisa-kusvibiswa nehutachiona senge aspergillus, hep B uye hutachiona hwakapararira kuburikidza neyemvura nitrogen medium (2,3)
• Mukana wekuti nitrogen yemvura ipinde mumidziyo panguva yekunyudzwa. Kana yabviswa mudura uye ichidziirwa kusvika kukamuri tembiricha, iyo nitrogen inokurumidza kuwedzera. Nekuda kweizvozvo, iyo vial inogona kupwanyika kana yabviswa kubva mumvura nitrogen kuchengetedza, ichigadzira njodzi kubva kune zvese zvinobhururuka marara uye kuratidzwa kune zviri mukati (1, 4).

Nezvikonzero izvi, ultra-yakaderera tembiricha chengetedzo inowanzo mumhute chikamu nitrogen. Kana samples dzichifanirwa kuchengetwa muchikamu chemvura, nyanzvi ye cryoflex tubing inofanirwa kushandiswa.

Iyo yakaderera kune chikamu chemhute ndechekuti yakamira tembiricha gradient inogona kuitika zvichikonzera kushanduka kwetembiricha pakati -135 ℃ uye -190 ℃. Izvi zvinoda kunyatsotariswa uye nekushingaira kutarisa kwemvura nitrogen mazinga uye tembiricha kusiyana (5).

Vazhinji vagadziri vanokurudzira kuti cryovials akakodzera kuchengetwa kusvika -135 ℃ kana kushandiswa muchikamu chemhute chete.

Kunyungudutsa Masero Ako Akachengetwa

Maitiro ekunyungudutsa anonetsa kune tsika nechando, uye kubata kwakaringana uye hunyanzvi hunodiwa kuti ive nechokwadi chekushanda, kupora, uye kushanda kwemaseru. Iwo chaiwo ekunyungudutsa maprotocol achaenderana nemhando dzemasero. Nekudaro, kukurumidza kunyungudika kunoonekwa seyakajairwa ku:

• Deredza chero kukanganisa kweserura kupora
• Batsira kuderedza kuratidzwa nguva kune solutes iripo mune inotonhora midhiya
• Deredza chero kukuvadzwa ne ice recrystallization

Mvura yekugezera, yekugezesa bhizi, kana midziyo yemagetsi yakasarudzika ndiyo inowanzo shandiswa kunyungudutsa sampuli.

Kazhinji kazhinji mutsara wesero 1 unonyungudutswa pane imwe nguva kwemaminetsi 1-2, nekutenderera zvinyoro nyoro mumvura yekugezera ye37 ℃ kusvika kwasara kachando kechando mubhodhoro isati yasukwa mune yekukura isati yadziya.

Kune mamwe maseru akaita semammalian embryos, kudziya zvishoma nezvishoma kwakakosha kuti vararame.

Maseru atogadzirira kuita sero tsika, kutsaurwa kwesero, kana mune haematopoietic stem masero - zvidzidzo zvekugoneka kuvimbisa kutendeseka kwevanopa stem masero pamberi pe myeloablative therapy.

Zvakajairika kuita kutora ma aliquots madiki emuenzaniso wakashambidzwa unoshandiswa kuita sero kuverenga kuona maseru ekuisa paplating mutsika. Iwe unogona ipapo kuongorora mhedzisiro yemasero ekuzviparadzanisa nevamwe uye kuona kuvepo kwesero.

Maitiro Akanakisa eKuchengeta kweCryovials

Kubudirira cryopreservation yemasampuli akachengetwa mu cryovials kunoenderana nezvinhu zvakawanda muprotocol zvinosanganisira kuchengetedza kwakaringana uye kuchengetedza rekodhi.

• Kupatsanura maseru pakati penzvimbo dzekuchengetera- Kana mavhoriyamu achibvumira, patsanura maseru pakati pemabhodhoro uye woachengeta munzvimbo dzakasiyana kudzikisa njodzi yekurasikirwa nemuenzaniso nekuda kwekutadza kwemidziyo.
• Kudzivirira kusvibiswa kwepakati-Sarudza kushandisa imwechete-yakaora cryogenic vials kana autoclave usati watevera kushandiswa
• Shandisa zvivharo zvakakura zvakafanira kumaseru ako- mabhodhoro anouya muhuwandu hwehuwandu pakati pe1 ne5mls. Dzivisa kuzadza mabhodhoro kuti uderedze njodzi yekutsemuka.
• Sarudza mukati kana kunze threaded cryogenic vials-Mabhodhoro emukati akaiswa shinda anokurudzirwa nemamwe mayunivhesiti kuti atore matanho ekuchengetedza - anogona zvakare kudzivirira kusvibiswa panguva yekuzadza kana kuchengetwa mumvura yenitrogen.
• Prevent Leakage-Shandisa bi-jekiseni zvisimbiso zvakaumbwa mune screw-cap kana O-mhete kudzivirira kubuda uye kusvibiswa.
• Shandisa 2D barcode uye label vials- kuti ive nechokwadi chekucherekedza, mabhodhoro ane nzvimbo huru dzekunyorera anoita kuti bhodhoro rega rega ringe rakanyorwa zvakakwana. 2D barcodes inogona kubatsira nekuchengetedza manejimendi uye kuchengetedza rekodhi. Makepisi ane mavara anobatsira pakuzivikanwa zviri nyore.
• Kuchengetedza kuchengetedza kwakakwana-Kuona kuti masero haarasikirwe, midziyo yekuchengetera inofanirwa kugara ichitarisa tembiricha uye mvura nitrogen mazinga. Ma alarm anofanirwa kuiswa kuti azivise vashandisi nezve kukanganisa.

Chengetedzo Yekuchengetedza

Liquid nitrogen yave tsika yakajairika mukutsvagisa kwemazuva ano asi inotakura njodzi yekukuvara kwakakomba kana ikashandiswa zvisirizvo.

Midziyo yekudzivirira yakafanira (PPE) inofanirwa kupfekwa kuderedza njodzi yechando, kupisa uye zvimwe zviitiko zvakashata kana uchibata mvura yenitrogen. Pfeka

• Cryogenic magirovhosi
• Laboratory jasi
• Impact resistant full face shield inovharawo mutsipa
• Shangu dzakavharwa
• Splashproof plastiki apron

Mafiriji enitrogen emvura anofanirwa kuiswa munzvimbo dzinofefeterwa nemhepo kuti kuderedze njodzi yekufemereka - yapukunyuka nitrogen inopfungaira uye inobvisa okisijeni yemumhepo. Zvitoro zvihombe zvemavhoriyamu zvinofanirwa kunge zvine low oxygen alarm system.

Kushanda vaviri vaviri kana muchibata mvura nitrogen kwakanakira uye kushandiswa kwayo kunze kwemaawa ekushanda akajairika kunofanirwa kurambidzwa.

Cryovials Kutsigira Basa Rako

Suzhou Ace Biomedical kambani inopa yakasarudzika sarudzo yezvigadzirwa zvinosangana neyako cryopreservation zvido zvemhando dzakasiyana dzemasero. Iyo portfolio inosanganisira huwandu hwemachubhu pamwe nemhando yeasina kubereka cryovials.

Cryovials yedu ndeiyi:

• Lab Kurukura Chivharo 0.5mL 1.5mL 2.0mL Cryovial Cryogenic Vials Conical Pazasi Cryotube neGasket

  ● 0.5ml, 1.5ml, 2.0ml tsanangudzo, ine siketi kana isina siketi
  ● Conical kana self stand dhizaini, sterile kana isina-sterile zvese zviripo
  ● Screw cap tubes yakagadzirwa nemedical grade polypropylene
  ● PP Cryotube Vials inogona kudzokororwa nechando uye kunyungudutswa
  ● Iyo yekunze chivharo dhizaini inogona kuderedza mukana wekusvibiswa panguva yekuenzanisira kurapwa.
  ● Screw cap cryogenic tubes Universal screw threads kuti ishandiswe
  ● Machubhu anokwana marotor akajairika
  ● Cryogenic chubhu o-mhete machubhu anokwana mwero 1-inch uye 2-inch, 48well, 81well, 96well uye 100well mabhokisi efiriza.
  ● Autoclavable kusvika 121°C uye inotonhora kusvika -86°C

  CHIKAMU NO	MATERIAL	VOLUME	CAPCOLOR	PCS/BAG	BAGS/CASE
  ACT05-BL-N	PP	0.5ML	Dema, Yero, Bhuruu, Tsvuku, Pepuru, Muchena	500	10
  ACT15-BL-N	PP	1.5ML	Dema, Yero, Bhuruu, Tsvuku, Pepuru, Muchena	500	10
  ACT15-BL-NW	PP	1.5ML	Dema, Yero, Bhuruu, Tsvuku, Pepuru, Muchena	500	10
  ACT20-BL-N	PP	2.0ML	Dema, Yero, Bhuruu, Tsvuku, Pepuru, Muchena	500	10