Maitiro ekushandisa PCR ndiro kuita kuyedza?

PCR (polymerase chain reaction) mahwendefa anoshandiswa kuita ongororo dzePCR, dzinoshandiswa zvakanyanya mukutsvagisa molecular biology kukwidziridza kutevedzana kweDNA.

Heano maitiro akajairika ekushandisa aPCR ndirozvechiyedzo chakajairika:

1. Gadzirira PCR yako reaction musanganiswa: Gadzirira PCR yako reaction musanganiswa zvinoenderana neprotocol yekuedza kwako, iyo inowanzo sanganisira template DNA, PCR primers, dNTPs, Taq polymerase, buffer uye zvimwe zvinowedzera.
2. Wedzera maitiro ekuita musanganiswa kuPCR ndiro: Uchishandisa yakawanda-channel pipette kana manual pipette, wedzera reaction mix kumatsime ePCR plate. Iva nechokwadi chekudzivisa kuunza mabhuru emhepo mumusanganiswa wekuita, sezvo izvi zvinogona kukanganisa migumisiro yekuedza kwako.
3. Wedzera yako template DNA kumusanganiswa wekuita: Zvichienderana nekuedza kwako, ungangoda kuwedzera yako template DNA kumusanganiswa wekuita. Kana iwe uri kushandisa yakawanda-channel pipette, iva nechokwadi chekushandura matipi pakati pemasample kudzivirira kusvibiswa kwemuchinjiko.
4. Nama ndiro: Kana wawedzera reaction mix uye template DNA kuPCR plate, namira ndiro nechisimbiso chakakodzera, senge PCR plate sealing film kana cap strip.
5. Isa ndiro mu thermocycler: Chekupedzisira, isa PCR ndiro yakavharwa mu thermocycler uye mhanyisa chirongwa chePCR chako, icho chinowanzo sanganisira nhevedzano yematembiricha inobvumira kuti DNA ikwidziridzwe.

Mushure mekuita kwePCR kwapera, unogona kuongorora zvigadzirwa uchishandisa nzira dzakasiyana siyana, senge gel electrophoresis kana kutevedzana. Ita shuwa yekutevera chaiwo maprotocol ekuedza kwako kuti uwane mhedzisiro uye yakavimbika.

Suzhou Ace Biomedicalinotungamira mugadziri wemhando yepamusoroPCR inoshandiswa. Isu takatsaurirwa kukupa maturusi akavimbika uye anoshanda ezviedzo zvako zvePCR, nemhando yezvigadzirwa zvakagadzirirwa kuzadzisa zvinodiwa nevaongorori mune dzakasiyana siyana.

Yedu PCR yekushandisa inosanganisiraPCR mahwendefa, PCR machubhu, PCR chubhu mitsetse, uye anoisa chisimbiso mafirimu. Zvese zvigadzirwa zvedu zvinogadzirwa nemhando yepamusoro-soro, kuve nechokwadi chekuti zvinokwanisa kumirisana nekuomarara kwePCR maitiro uye kuburitsa zvinowirirana uye zvakarurama mhedzisiro.

PaSuzhou Ace Biomedical, isu tinonzwisisa kukosha kwekurongeka mune yako PCR kuyedza. Ndosaka zvigadzirwa zvedu zvePCR zvichigadzirwa nemhando yepamusoro yemhando uye kuongororwa kwakasimba kuti ive nechokwadi chekuti zvinosangana kana kudarika zvaunotarisira. Zvigadzirwa zvedu zvakare zvakagadzirirwa kuti zvienderane neyakasiyana siyana ye thermocyclers, zvichiita kuti ive sarudzo inogoneka kune vanotsvaga mumalabhu akasiyana.

Kunyangwe iwe uri kuita tsvakiridzo yekutanga, kuongororwa kwekiriniki, kana zvimwe zvikumbiro, Suzhou Ace Biomedical ine PCR inobatika yaunoda kuti uwane zvinangwa zvako. Takazvipira kupa zvigadzirwa zvemhando yepamusoro uye sevhisi yevatengi, uye tinodada kuva shamwari yakavimbika yevaongorori pasi rese.

Bata nesu nhasi kuti udzidze zvakawanda nezve PCR yedu yekushandisa uye kuti tingatsigire sei tsvagiridzo yako.