UNGAMISA SEI KUSANGANISA 96 YAkadzika ZVAKANAKA ndiro

Imaawa mangani pasvondo aunorasikirwa nendiro dzetsime rakadzika?

Kurwisana ndekwechokwadi. Hazvina mhosva kuti mapipette mangani kana mahwendefa awakatakura mukutsvagisa kwako kana basa, pfungwa dzako dzinogona kutanga kutamba matangi pauri kana zvasvika pakurodha iyo inotyisa 96 yakadzika tsime ndiro.

Zviri nyore kuwedzera mavhoriyamu kutsime risiri iro kana mutsara usiriwo. Zviri nyore kuita netsaona kupeta ndiro imwe yakadzika yetsime.

Kana kuti unorodha sample yese isiriyo mumatsime akawanda, zvichikudyira maawa ebasa.

Kana, pamwe wakaita zvese nemazvo, asi unotanga kechipiri kuzvifungira. Kutanga patsva.

Nguva yako yakakosha. Mareagents ako akanyanya kukosha. Uye, zvinonyanya kukosha, data rako rakanyanya kukosha.

Hatifanirwe kukuudza kuti kutambisa nguva uku, kana iwe uchiwanzo gadzira reagents nekusanganisa. Uyezve, hazvinzwe zvakanyanya padanho rekuvimba zvakare.

Heano akanakisa matipi uye matipi kubva kune vamwe iwe aunogona kutanga kubatanidza mune yako lab maitiro.

Chii chinonzi 96 tsime rakadzika ndiro?

Chinhu chinowanzo kufuratirwa mumaLab uye nzvimbo dzekutsvagisa kwese kwese, mahwendefa akadzika matsime akanakira kwenguva pfupi uye kwenguva refu sampuli yekuchengetedza, kugadzirira, uye kusanganisa. Vanogona kuva neskweya tsime kana pasi rakatenderera.

Kushandiswa kwavo kunosiyana, asi ivo vanowanzo shandiswa muhupenyu sainzi mashandisirwo uye kushandiswa kwekutsvagisa, kusanganisira:

• Tissue cell tsika basa uye kuongorora masero
• Enzyme assays
• Zvidzidzo zveproteomics
• Reagent reservoirs
• Safe sampuli yekuchengetedza (kusanganisira cryogenic kuchengetedza)

Mazano epamusoro & matipi ekukunda 96 yakadzika tsime ndiro kukanganisa

Isu takanyora rondedzero yepamusoro masisitimu uye maitiro kubva kune vaunoshanda navo:

1. Tarisa pfungwa dzako uye ramba wakatarisa:Sezvimwe chero chinhu muhupenyu, kukanganisa kunowanzo kuitika kana wakaneta, kushushikana, kana kutsauswa (… kana zvese zviri pamusoro). Rega kunetseka nezvekumhanyisa basa rako. Zvinonoke, uye funga nezvenhanho imwe neimwe zvishoma. Uye ramba wakatarisa. Kutaura nekushanda kunoita kuti mamwe mabasa afambe nekukurumidza, asi kwete nebasa iri. Vamwe vaongorori vanorembera “Kwete kutaura” kusaina sezvo vari pakati pebasa iri. Mimhanzi yekuzorodza (kunyanya zviridzwa) inokurudzirwa kana iwe uchida ruzha rwekumashure paunenge uchishanda!
2. Nyanisa matipi ako epipette nematsime anoenderana:Iyo nyowani pipette bhokisi ndiyo yakanakisa kune yakadzika tsime ndiro. Matanidza tsime nebhokisi paunenge uchienda. Iva nebhokisi rekuchengetedza pane standby kana iwe ukapera, saka haufanirwe kukanganisa system yako kana iwe uchida zvimwe. Shandisa matipi epipette kuti uchengetedze kuverenga zvakanaka.
3. Nyora kunze:Gadzira pepa reExcel rekusanganisa master, uye 96 yakadzika tsime ndiro mepu. Tsime rega rega rine zita rekutanga uye samples. Rongedza yako ese master musanganiswa nenzira ine musoro, uye kodhi kodhi yega yega yekutanga seti (kana uchishandisa anopfuura imwe). Huya nepepa iri murabhoritari, uye tarisa maka pepa paunenge uchienda. Iwe unogona zvakare kunyora mari yeagent pane post-iyo uye woichengeta padivi pako sekiyi yako yemuenzaniso paunenge uchitakura. Sarudza hurongwa hwekushanda kuburikidza nadzo (semu alfabheti kana nhamba, zvichienderana nemanyorerwo adzinoitwa) uye usambotsauka kubva pahurongwa hwako. Paunenge uchigadzira musanganiswa, isa zvese zvakarongeka pane rack yako, wozoiendesa kukona iri kure kana wapedza.
4. Tepi ishamwari yako itsva yepamoyo:Tepi kubva kuzere kwendiro, parutivi kubva munzvimbo yauri kushingaira kurodha. Shanda mundiro nenzira iyi, uchifambisa tepi nguva imwe neimwe chikamu chapera. Unogona kunyora tepi yako (semuenzaniso A – H, 1 – 12) kuti ikubatsire kuramba uri mugwara.
   Semuyenzaniso, paunenge uchiisa Gene A mastermix mumakoramu 1 uye 2 yendiro yako yakadzika yetsime, tanga watora tepi wovhara zvinyoro nyoro makoramu 3 ne4. Unogona kutoita iyi koramu imwe panguva, kuti ugare wakarongeka. Inobatsira kuramba wakatarisa panguva yakaoma yepakati matsime. Ingoyeuka kubata ndiro pasi zvishoma nezvishoma paunenge uchibvisa tepi yako, kuti usaputika.
5. Ramba nazvo:Kana iwe ukaona kuti system yako haisi kushanda, usachinje pakati. Chishandure usati kana shure, asi usambofa uri pakati (zvinotungamira kunyonganiso yakawandisa!).
6. Dzidzira:Ramba uchienderana nemaitiro aunosarudza. Kuita nhanho idzi kundangariro dzemhasuru kunotora nguva, asi nekufamba kwenguva iwe unofanirwa kutanga kuona kuvandudzwa kwakakosha pabasa rako (uye zvakanyanya kuderera kushushikana munzvimbo yako yebasa!)

Sarudza midziyo yakakodzera:

Kubva pamidziyo kusvika kune yemhando, matsime akatenderera kana pasi peconical, pane akasiyana sarudzo kana uchiodha 96 yakadzika tsime ndiro.

Zvimwe zvekufungidzira zvinosanganisira:

• Material: Ndeapi masampuli auri kushandisa? Ko tsime rako rakadzika rinoda kuvharwa kana kuti siliconized?
• Saizi: Yakawanda sei vhoriyamu inoda kukwana mune yako yakadzika tsime 96 PCR ndiro?
• Tembiricha: Ndeapi tembiricha ayo matsime ako akadzika anoda kumira?
• Ndeapi masimba ekuisa centrifugation anogona 96 yako yakadzika tsime ndiro inomira?

Hezvino izvo masayendisiti mazhinji ari kushandisa kune zvakajairika application:

Aya akareruka 96 Deep Well Plates

Aya mabhodhoro akadzika mabhodhoro anobatsira sei maLab uye maneja eLab:

• Annzira iri nyorekuunganidza nekugadzirira samples (sezvo pasina kushomeka kwezvinhu izvo zvinoitika murabhoritari yako mazuva ese)
• Tora yakakosha labspace kumashure, ine yakasimba stacking inoitisa kuti zvive nyore kuchengeta kupfuura kare
• Dzivisa kurashikirwa neyakavandudzwa kusanganisayemasampuli ako madiki emvura
• A design kutiinoderedza kuchengetedza kumadziro, saka unorasa zvishoma zvemuenzaniso wako
• Pay33% zvishomakupfuura zvaungaite kune mamwe mabhureki anotungamira

Zvimiro zvinosanganisira:

• Zasi rakatenderera
• Inogona kuomeswa nechando kana mufiriji (kusvika -80 C)
• Kugadzikana - havazoiti nezvinonyungudutsa mundiro
• Usasanganise nesimbi inorema kuti ivandudzwe zvakachengeteka
• Yakagadzirwa zvinoenderana nepasi rose chiyero saizi (SBS), ichiita kuti ive yakakodzera kune otomatiki workstations
• Bvumira kusachengetedzwa kwemvura kwemuenzaniso wako kumadziro

Kusarudza ndiro yakanaka yetsime kunogona kukubatsira kudzivirira:

• Mapoinzi edata akapotsa
• Sample rerun
• Kufamba zvishoma nezvishoma
• Wakapotsa mazuva ekupedzisira eprojekiti

Kufara kutsvakurudza

96 mahwendefa akadzika matsime anowanikwa mumalabhu nenzvimbo dzekutsvagisa pasirese. Ivo vanogona kuchengetedza nguva, kushanda nesimba, uye nzvimbo yekuchengetera, asi yakakodzera sisitimu yakakosha paunopedza basa rako.

Kubva pakuwedzera kwekuchengetedza, kusvika pakuwedzeredzwa musanganiswa, mahwendefa akadzika matsime akanakira kusanganisa chemistry uye maapplication eraibhurari, anopesana nemakemikari mazhinji, zvinonyungudutsa, uye madoro anoshandiswa mucombinatorial chemistry.

Yakanakira kuunganidza sampuli, gadziriro yemuenzaniso, uye yenguva refu (kana yenguva pfupi) chengetedzo yemuenzaniso, mahwendefa emvura akadzika uye mameti ekuisa chisimbiso anogona kuvandudza mafambiro ebasa, uye iyo yekurudyi yakadzika tsime ndiro ichakubatsirawo kuburitsa data remhando yepamusoro rezvishandiso zvakajairika mukati. Sayenzi yehupenyu (uye mberi).