Nhanganyaya
PCR mahwendefa, chakanyanya murabhoritari kwemakore mazhinji, chiri kuramba chichiwedzera muchimiro chemazuva ano sezvo marabhoritari anokwidziridza mabudisiro awo uye achiwedzera kushandisa otomatiki mukati mekufamba kwebasa ravo. Kuzadzikisa zvinangwa izvi tichichengetedza huchokwadi nekuvimbika kwezviedzo kunogona kuve kwakaoma. Imwe yenzvimbo dzakajairika umo kukanganisa kunogona kupinda mukati ndeye kuiswa chisimbiso kwePCR mahwendefa, nemaitiro asina kunaka anobvumira kubuda kwemasampuli, kushandura pH uye nekudaro kukanganisa enzymatic mabasa, uye kukoka kusvibiswa. Kudzidza kuisa chisimbiso aPCR ndironemazvo inobvisa njodzi idzi uye inova nechokwadi chekuberekazve.
Tsvaga Chisimbiso Chakakodzera chePCR Plate yako
Plate Caps vs. Film Seals vs. Lids
Capsinzira yakanaka yekusimbisa ndiro yako nechisimbiso chakasimba, ichiri kukupa iwe kuchinjika kusunungura zviri nyore uye kuvhara ndiro zvakare sezvaunoda pasina chero tsvina. Nekudaro, macaps ane akati wandei akakosha madhiri.
Chekutanga, iwe uchafanirwa kutenga iyo chaiyo cap inoenderana, izvo zvinoita kuti dzisaite zvakasiyana. Iwe uchafanirwa kuve nechokwadi chekuti chivharo chaunosarudza chinoenderana neplate, iyo inotsamira kune mugadziri wayo, uye sarudza ine domed kana flat zvichibva pane thermocycler yauri kushandisa.
Chechipiri, kushandisa macaps mundiro kunogona kudzokorora uye kunetesa, nenjodzi yekusvibiswa-muchinjika kana iwe ukaisa chivharo chisiri patsime risiri iro.
Nepo zvisimbiso zvemufirimu zvisinganyanyi kuchinjika maererano nekubvisa uye kutsiva, zvinogoneka zvakanyanya sezvo zvichikwana chero mhando yePCR plate, pasina hanya kuti ndiani mugadziri. Dzinogona kungochekwa kusvika pakukura, zvichiita kuti dzinyatsoshanda.
Imwe sarudzo ivharo yeplate. Izvi zvinopa dziviriro shoma iyo zvivharo uye zvisimbiso, uye zvinonyanya kushandiswa kwenguva pfupi yekuvharira kudzivirira kusvibiswa.
Optical vs. Foil Film Seals
Kunyangwe iwe uchida optical, clear seal kana analuminium foil filmkuvhara ndiro yako kunosarudzwa nefomati yako yekuedza.Optical sealing mafirimuzviri pachena kuti zvikubvumire kuona masampuli, uchiri kuadzivirira uye kudzivirira kubuda. Iwo anonyanya kubatsira mukuedza kweqPCR zvinosanganisira kuita zvakanyatsojeka zviyero zvefluorescence zvakananga kubva mundiro, mune iyo kesi iwe unozoda yekuvharisa firimu inosefa kunze sediki fluorescence sezvinobvira. Zvakakosha kuve nechokwadi chekuti chisimbiso kana chivharo chauri kushandisa chine mwero wakakwana wekujeka kwemaziso kuti uve nechokwadi chekuti kuverenga kwakaringana.
Mafirimu efoiri akakodzera chero sampuli dzinenge dzakareruka kana dzinofanira kuchengetwa dziri pasi pe80°C. Nechikonzero ichi, akawanda masampuli akagadzirirwa kuchengetedza kwenguva refu anoda foil firimu. Mafirimu emafoiri anogona kuboorwawo, ayo anobatsira pakuongorora matsime ega ega, kana kuendesa sampuli netsono. Izvi zvinogona kuitika nemaoko kana sechikamu cherobhoti chikuva.
Uyezve funga kuti zvinhu zvine hukasha zvinosanganisira acids, mabhesi kana zvinonyungudutsa zvinoda chisimbiso chinogona kushingirira pazviri, apa chisimbiso chefoiri chingangove chakakodzera.
Adhesive vs. Kupisa Chisimbiso Firimu
Adhesive film sealszviri nyore-mberi uye zviri nyore kushandisa. Zvese zvaunoda ndezvekuti mushandisi aise chisimbiso mundiro, uye shandisa iri nyore applicator chishandiso kutsikirira pasi uye kugadzira chisimbiso chakasimba.
Zvisimbiso zvekupisa zvakanyanya kumberi, zvichipa chisimbiso chenguva refu icho chakaderedza mazinga evaporation zvichienzaniswa neyakajairwa chisimbiso chekunamatira. Iyi sarudzo yakakodzera kana iwe uri kutsvaga kuchengeta samples munguva refu, kunyangwe izvi zvichiuya nechimwe chinodiwa chemidziyo yekuisa chisimbiso cheplate.
Maitiro ekusimbisa PCR Plate
Plate Chisimbiso Nzira
Self-Adhesive
1. Ita shuwa kuti uri kushanda pane flat uye yakagadzikana worksurface
2. Bvisa firimu kubva pakuputira kwayo, uye bvisa kutsigirwa
3. Nyatsoisa chisimbiso pandiro, uchiona kuti matsime ose akavharwa
4. Shandisa mudziyo wekushandisa kushandisa kumanikidza mhiri kwendiro. Tanga kubva kune rumwe rutivi uye shandisa nzira yako kuenda kune imwe, uchidzvanya zvakaenzana
5. Dzokorora izvi kakawanda
6. Mhanyai applicator yako kumativi ekunze matsime, kuti uone kuti aya akanyatsonamwawo.
Kupisa Zvisimbiso
Zvisimbiso zvinopisa zvinoshanda nekunyungudusa firimu kumucheto wetsime rimwe nerimwe, nerubatsiro rweplate sealer. Kuti ushandise kupisa sealer, tarisa kune mirairo inopihwa nemugadziri wemidziyo. Ita shuwa kuti mugadziri waunotora mudziyo wako ane mukurumbira, sezvo zvakakosha kuti chisimbiso chakakodzera, chinoshanda uye chisina mvura.
Plate Kuvhara Mazano Epamusoro
a. Paunenge uchimanikidza chisimbiso, enda mune ese akachinjika uye akachinjika nzira kuti uve nechokwadi chekuisa chisimbiso chakakodzera.
b. Inogara yakanaka tsika yekumhanyisa bvunzo yezvese zvauri kuita, uye izvi hazvina kusiyana nekuisa chisimbiso cheplate. Edzai nendiro isina chinhu usati washandisa imwe ine sampuli.
c. Paunenge uchiyedza, bvisa chisimbiso uye tarisa kuti uone kuti chinonamira chakanamira pasi nemazvo, pasina mapundu. Pane ratidziro inooneka yeizvi mugwaro rereferenzi rekutanga. Kana iwe usina kuvhara ndiro yacho zvakanaka, paunobvisa chisimbiso pachava nemapopo apo iyo yakanamatira haina kuita chisungo chakazara kune ndiro.
d. Nekutumira uye kutakura sampuli, unogona kuona zvichibatsira kuisa chisimbiso chepurasitiki pamusoro pechisimbiso chefoil kuti uwedzere kuchengetedzwa (kunyanya kubva pakuboora).
e. Gara uine chokwadi chekuti hapana mapundu kana makwinya paunenge uchiisa firimu - izvi zvinokonzeresa kuvuza uye kubuda.
Nguva yekutumira: Nov-23-2022