Kana pombi mavhoriyamu kubva pa0.2 kusvika ku5 µL, kurongeka kwepombi uye kurongeka kwakakosha zvakanyanya nzira yakanaka yekupopa yakakosha nekuti kubata zvikanganiso zvinonyanya kuoneka nemavhoriyamu madiki.
Sezvo kunyanya kutariswa kuri kuiswa pakudzikisa reagents uye mutengo, mavhoriyamu madiki ari kudiwa zvakanyanya, semuenzaniso, pakugadzirira kwePCR Mastermix kana enzyme reactions. Asi kupinza mavhoriyamu madiki kubva ku0.2 - 5 µL inoseta matambudziko matsva ekurongeka kwepombi uye nemazvo. Mapoinzi anotevera akakosha:
- Pipette uye saizi yetipi: Gara uchisarudza iyo pipette ine yakaderera zita vhoriyamu inogoneka uye diki muromo kuti uchengete cushion yemhepo idiki sezvinobvira. Pakuisa pombi 1 µL eg, sarudza 0.25 - 2.5 µL pipette uye inofananidzwa nepipeti kwete 1 - 10 µL pipette.
- Calibration uye kugadzirisa: Izvo zvakakosha kuti mapipette ako anyatso kurongeka uye achengetedzwe. Zvigadziridzo zvidiki uye zvikamu zvakaputsika pane pipette zvinotungamira kuwedzera kukuru kwemaitiro akarongeka uye asina kukanganisa maitiro. Calibration inoenderana neISO 8655 inofanirwa kuitwa kamwe chete pagore.
- Positive displacement pipettes: Tarisa uone kana uine yakanaka displacement pipette ine yakaderera vhoriyamu renji mulabhu yako. Muzhinji, kushandisa rudzi urwu rwepipette kunotungamirira kune imwe nzira yepipetting iri nani maererano nekururama uye kurongeka kupfuura ne classic air-cushion pipettes.
- Edza kushandisa mavhoriyamu akakura: Unogona kufunga kudzikisa sampuli yako kune pipette mavhoriyamu makuru ane huwandu hwakafanana mukuita kwekupedzisira. Izvi zvinogona kuderedza zvikanganiso zvepipetting nemavhoriyamu mashoma emuenzaniso.
Pamusoro pechishandiso chakanaka, muongorori anofanira kunge aine nzira yakanaka yekupomba. Nyatsoteerera kune anotevera matanho:
- Zano rekubatanidza: Usajame iyo pipette pamuromo sezvo izvi zvinogona kukuvadza iyo yakanaka yepipi yekupedzisira kuita kuti danda remvura ridzoserwe kana kukuvadza orifice. Ingo shandisa chiedza chechiedza paunenge uchibatanidza chipi uye shandisa pipette ine chitubu-yakatakura tip cone.
- Kubata pipette: Usabata pipette muruoko rwako uchimirira centrifuge, cycler, etc. Mukati wepipette uchapisa uye utungamirire mutsara wemhepo kuti uwedzere zvichikonzera kutsauka kubva pahuwandu hweseti kana pipetting.
- Pre-wetting: Iyo humidification yemhepo mukati memuromo uye pipette inogadzirira chidimbu chemuenzaniso uye inodzivirira kubuda kwemhepo kana uchishuvira vhoriyamu yekufambisa.
- Vertical aspiration: Izvi zvakakosha zvakanyanya kana uchibata mavhoriyamu madiki kudzivirira iyo capillary effect inoitika kana pipette inobatwa pakona.
- Kunyudza kudzika: Nyura iyo muromo zvishoma sezvinobvira kudzivirira mvura kupinda mumuromo nekuda kwecapillary effect. Mutemo wechigunwe: Iyo idiki muromo uye vhoriyamu, inodzika kudzika kunyudza. Isu tinokurudzira huwandu hwe2 mm paunenge uchiputira mavhoriyamu madiki.
- Kuparadzira pa 45 ° angle: Yakanyanya kuyerera-kunze kwemvura inovimbiswa kana pipette inobatwa pa45 ° angle.
- Kubata kumadziro emudziyo kana pamusoro pemvura: Mavhoriyamu madiki anogona chete kugovaniswa zvakanaka kana muromo wakabatwa pamadziro emudziyo, kana kunyudzwa mumvura. Kunyangwe kudonha kwekupedzisira kubva kumuromo kunogona kugoverwa nemazvo.
- Kufuridza-kunze: Kufuridza-kunze kunosungirwa mushure mekupa mavhoriyamu akaderera kuburitsa kunyangwe donhwe rekupedzisira remvura iripo mumuromo. Kuputika kunofanirawo kuitwa kumadziro emudziyo. Chenjerera kuti usaunza mabhuru emhepo mumuenzaniso paunenge uchiita kuputika-kunze kwemvura.
Nguva yekutumira: Feb-18-2021