Maitiro Ekupa Kwemavhoreti Zvikuru Zvekutamba Nyowani Mabhande Pipi

Sezvinonyanya kutarisisa zviri kuiswa pakudzora reagents uye mutengo, madiki mavhoriyamu ari mukunyorwa kwakanyanya, semuenzaniso, kuti gadziriro yePCT mastermix kana enzyme maitiro. Asi pipetting madiki mavhoriyamu kubva ku0.2 - 5 μl inoisa matambudziko matsva ekurongeka kwe pombetting uye chaiyo. Pfungwa dzinotevera dzakakosha:

1. Pipette uye sif size: Gara uchisarudza pipette neiyo yakaderera zita rinonzwisisika rinogona uye diki diki yekuchengeta iyo cushion sechidiki sezvinobvira. Kana pipetting 1 μL eg, sarudza iyo 0.25 - 2.5 μl pipeette uye kufananidza tipi pane 1 - 10 μl pipiette.
2. Calibration uye kugadzirisa: Izvo zvakakosha kuti mapipi ako akanyatsotaurwa uye akachengetwa. Diki gadziriso uye zvikamu zvakaputswa pane pipette zvinotungamira mukuwedzera kukuru kwehurongwa hwehurongwa uye hwakarongeka hwekukanganisa. Calibration zvichienderana naIso 8655 inofanira kuitwa kamwe chete pagore.
3. Pipitalating pombi yePipettes: Tarisa kana iwe uine yakanaka yekudhura pipette ine yakaderera vhoriyamu huwandu mune yako lab. Kazhinji, kushandisa iyi mhando yePipette inotungamira kune iri nani pipetting mhedzisiro maererano nekunyatsojeka uye nekunyatsopfuura uye neyekutamba kwemhepo-cushion pombi.
4. Edza kushandisa yakakura kuvimbisa: Iwe unogona kufunga kudzikisira yako sampuli kuti pipette yakakura mavhiyamu ine huwandu hwakaenzana mukuita kwekupedzisira. Izvi zvinogona kuderedza zvikanganiso zve pombetting neiri diki sampuli vhesi.

Kuwedzera kune chishandiso chakanaka, muongorori anofanira kunge aine yakanaka kwazvo pombix maitiro. Teerera Zvakakosha kune Inotevera Matanho:

1. Zano Attardment: Usatambe pombi pane iyo tip sezvinoita izvi zvinogona kukuvadza iyo yakanaka tip magumo kukonzera iyo yemvura beam kuti ridzorerwe kana kukuvara orifice. Ingoshandisa kudzvinyirira kwechiedza paunenge uchinamatira tip uye shandisa pipette neyechirimo-yakatakurirwa tip cone.
2. Kubata pipette: Usabate pipette muruoko rwako uchimirira iyo centrifuge, cycler, etccer yemukati yekupisa uye kutungamira iyo yemhepo cushion kuti uwedzere kuiswa kubva kuhuwandu hwekutsauka kubva kune seti vhoriyamu kana pipetting.
3. Pre-kunyorovesa: Iko kusimira kwemhepo mukati mechiputi uye pipette inogadzirira tipi yemuenzaniso uye inodzivisa kuvharidzira apo kushinha kweichinjisa.
4. Vertical kushuvira: Izvi zvakakosha pakubata mavhoriyamu madiki kuti udzivise iyo capillary mhedzisiro inoitika kana pipeette iyo pipeette inoitirwa pakona.
5. Kunyudzwa kwakadzika: Nyura iyo tipi zvishoma sezvinobvira kudzivirira mvura ichipinda iyo tip nekuda kweiyo capillary mhedzisiro. Kutonga kwechigunwe: Iyo diki iyo tip uye vhoriyamu, iyo yakadzika yakadzika yakadzika. Isu tinokurudzira huwandu hwe2 mm kana pipetting madiki madiki.
6. Kuparadzanisa pa45 ° Anga: Yakakwana kuyerera-kubva mumvura inovimbiswa kana pipeette iyo pipeji inoitirwa paAkaya.
7. Bata madziro emudziyo kana mvura yemvura: madiki madiki anogona chete kugoverwa zvakanaka kana tip ichirwiswa kurwisa rusvingo rwechikepe, kana yakanyudzwa mumvura. Kunyangwe iko kwekupedzisira kudonha kubva kune tip kunogona kugoverwa chaizvo.
8. Kurova-kunze: Kurova-kunze kunosungirwa mushure mekurasa mavhoriyamu akaderera kuti ubvise kunyange donhwe rekupedzisira remvura riripo mumupendero. Kurova-kunze kunofanirawo kuitwa kurwisa rusvingo rwechikepe. Chenjera kuti urege kuunza mhepo mabhuru mumuenzaniso kana uchiita kuputika-kunze kune mvura.