Automated pipettingndiyo imwe yedzakanakisa nzira dzekudzikisa kukanganisa kwevanhu, kuwedzera humbowo uye huchokwadi, uye nekumhanyisa kufambiswa kwebasa. Nekudaro, kusarudza nezve "zvinofanira-kuve" zvikamu zvekubudirira workflow otomatiki yemvura kubata zvinoenderana nezvinangwa zvako uye mashandisiro. Ichi chinyorwa chinokurukura mamwe emapoinzi akakosha ekufunga nezvazvo paunosarudza mvura inobata chikuva cherabhoritari yako.
Automating pipetting inhanho yakakosha mukuvandudza marabhoritari workflows, kubatsira kuwedzera kuberekana, kuwedzera kuburitsa, uye kuderedza zvikanganiso. Marabhoritari anoenderana neautomated liquid inobata matekinoroji emhando dzakasiyana dzemashandisirwo, kusanganisira gadziriro yemuenzaniso, kubviswa kweDNA, maseru-based assays, uye ELISAs. Aya mapuratifomu ndeyekudyara kwenguva refu uye anofanirwa kusarudzwa zvichibva kwete pane zvanhasi chete, asiwo izvo zvingave zvishuwo zverabhoritari. Izvi zvichava nechokwadi chekuti puratifomu chaiyo inosarudzwa, uye inogona kushanda murabhoritari zvinobudirira kwemakore mazhinji ari kuuya.
Matanho ekutanga
Usati waita chero sarudzo, nyatso tarisa maitiro ekuve otomatiki:
Uri kutanga nehurongwa hwakasimba here?
Liquid inobata otomatiki inogona kuvandudza zvakanyanya mafambiro ebasa, asi haigone kugadzirisa assay isati yatoshanda. Dhonza mafambiro ako pasi kuita matanho ega ega, uye funga nezve mhedzisiro yeimwe neimwe pakufamba kwese kwese. Semuenzaniso, kutora assay kubva kune manually pipetted, tube-based format kune automated, yakakwirira-density, plate-based workflows zvinoreva kuti samples uye reagents zvichange zviri padheki kwenguva yakareba. Izvi zvingakanganisa sei kutendeseka kwemasampuli ako uye reagents?
Zvaunoda zvichashanduka sei?
Kuti uchengetedze mari, zvingave kuyedza kuisa mari muhurongwa hunongosangana nezvinodiwa nerabhu yako zvazvino, asi nekufamba kwenguva unogona kurasikirwa. Funga kuti ndezvipi zvinhu zvakakosha, uye ndezvipi zvingave zvakanaka kuva nazvo. Iyo yakanaka otomatiki yemvura inobata sisitimu inofanirwa kugadziridzwa zvakare kuitira kuti iwe ugone kutora kune mitsva maapplication uye workflows sezvinodiwa shanduko. Iine inochinjika, modular sisitimu, zvakawanda zvinhu zveazvino workflows zvinogona kudzokororwa uye kukwidziridzwa.
Pane here mhinduro isiri pasherufu inoenderana nezvido zvako?
Mamwe masitesheni ekushanda akagadziridzwa kune chaiwo maapplication ane akapupurirwa maprotocol, senge DNA kubviswa, gadziriro yemuenzaniso, uye sero tsika. Izvi zvinogona kurerutsa maitiro ako ekusarudza, uye zvichingopa inobatsira "musimboti" chikamu chekubatanidza mune yakakura sisitimu mune ramangwana. Off-the-sherufu mhinduro dzakagadzirwa nekubatana kweramangwana uye kuchinjika mupfungwa zviri nani kune isingachinjiki, "yakavharwa" mapuratifomu.
Iwe une nzvimbo yakawanda sei, uye uri kuishandisa nemazvo here?
Nzvimbo inowanzova chinhu chinokosha. Mazhinji emvura ekubata masisitimu ave akawanda ekushandisa, izvo zvawedzera kudiwa kwekuchinjika uye nekuvandudza kushandiswa kwenzvimbo. Funga kusarudza otomatiki chikuva chinogona kuwana nzvimbo pazasi petafura yekushanda kuti usvike, semuenzaniso, yekuwedzera yekuongorora kana sampuli yekugadzirira michina, nezvimwe.
Zviri nyore sei kuchengetedza nekushandira?
Usakanganwa sevhisi uye kugadzirisa. Kusununguka kwekuwana nevanyanzvi kunogona kuderedza nguva yekudzikira uye kukanganisa kune yako mafambiro ebasa.
Kusarudza hardware chaiyo
Kunyangwe iwe uri kushanda mu genomics, cell biology, kuwanikwa kwezvinodhaka, molecular diagnostics, kana chimwe chinhu chakasiyana zvachose, iyo chaiyo mvura inobata system inogona kuita kuti hupenyu hwako huve nyore. Zvikumbiro zvakakosha zvinosanganisira:
Air kana liquid displacement pipetting?
Kutamiswa kwemhepo kwakanakira kugovera pamusoro pehukuru hwevhoriyamu, kubva pa0.5 kusvika ku1,000 μL. Kunyangwe ichingoenderana nemazano anoraswa, izvi zvinowedzera kukurumidza uye kugadzirwa nekubvisa matanho ekuwedzera ane chekuita nepipetting yemvura yekuchinjisa kana uchichinja zvinwiwa kana kutsvaira sisitimu. Inoderedzawo njodzi yekusvibiswa-uye inopa nzira yakachengeteka yekubata radioactive kana biohazardous zvinhu.
Liquid displacement inoenderana neakagadzika uye anoraswa matipi, uye ndiyo inosarudzirwa tekinoroji yeakawanda mavhoriyamu ari pasi pe5 μL. Washable fixed simbi matipi akanakira maapplication uko machubhu anoda kuboorwa kana kuti positive pressure pipetting inodiwa. Kuti ukwanise kuchinjika zvakanyanya, funga sisitimu inosanganisira zvese mhepo uye mvura inobviswa.
Ndeapi mavhoriyamu uye mafomati aunoshanda nawo?
Ita shuwa kuti chikuva chinogona kubata anodiwa epipetting mavhoriyamu uye mafomati erebhurabhu (machubhu uye mahwendefa) anowanzo shandiswa mulabhu yako. Uyezve funga kana otomatiki ichizobvumira diki sampuli uye reagent mavhoriyamu kuti ashandiswe, ichipa inogona mari yekuchengetedza.
Ndeapi maoko epombi aunofanira kusarudza?
Mhando huru ndeiyi 1) mapipette anochinja-chinja-kazhinji 1- kusvika ku8-channel-inokwanisa kubata machubhu, mahwendefa, uye mamwe akawanda mafomati e-labware; uye 2) multichannel maoko akagadzirirwa zvakananga kugovera mumahwendefa akawanda-tsime. Masisitimu echizvino-zvino anobvumira misoro yepombi kana madhizaini maplate kuti achinje "pane nhunzi" - sarudzo yekuchenjera yezvibvumirano zvinoshandisa zvinhu zvakawanda zvakasiyana, senge tsono dzakagadzika, matipi anoraswa, maturusi epini akaderera, nezvimwe.
Unoda maoko emarobhotinokutikuwedzera kuchinjika?
Maoko eRobotic gripper anopa kuchinjika kwakanyanya nekufambisa labware kutenderedza dhizaini yebasa. Maoko eRobhoti anogona kushandura "minwe" yavo inokurumidza kugonesa kuchinjika uye kubata kwakachengeteka kune ese machubhu uye mahwendefa.
Ndeupi rudzi rwepipetti tipi inozowedzera kuberekana?
Tip mhando ndiyo yakakosha inobatsira mukuberekana uye inogona kugadzira kana kutyora mashandiro ehurongwa. Mazano anoraswa anowanzo onekwa sesarudzo yakanakisa yekubvisa kusvibiswa kwepakati pakati pezvinyorwa zvebiological samples. Vamwe vatengesi zvakare ikozvino vanopa akakosha akaderera-vhoriyamu matipi akasimbiswa kune yakavimbika kugovera paiyo microliter kana submicroliter mazinga anodiwa kune maapplication akadai seassay miniaturization. Funga kutenga iyo otomatiki mutengesi mhando yepipette matipi kuti uve nechokwadi chekuti unowana mhedzisiro yakavimbika.
Zviridzwa zvinoshandisa matipi akagadziriswa zvinogona kunge zvine zvakanakira maererano nemutengo wekushandisa. Simbi dzakagadziriswa tsono dzinowanzosvika pasi pemidziyo yakadzika zviri nani pane matipi anoraswa, uye inogona zvakare kubaya septa. Yakanyatsogadzirirwa mazano ekugezesa zviteshi zvinoderedza njodzi yekuyambuka-kusvibiswa neseta iyi.
Unoda matipi anovimbiswa steril?
Kuti uderedze njodzi yekusvibiswa, shandisa chete zvinodyiwa zvakanyorwa kuti "zvisina kuchena." Izvi zvinogadzirwa pasi pemamiriro akaomesesa uye zvinoenderana nekurongedza uye zviyero zvekutakura izvo zvinova nechokwadi chetip sterility nzira yese kuenda kubhenji rebhu. Zvigadzirwa zvakanzi "presterile" hazvina mbereko kana zvichisiya mugadziri, asi zvinosangana nemikana yakawanda yekusvibiswa gare gare.
Software zvine basa
Software inopa iyo interface nemunhu anomisikidza uye nekushandisa chiridzwa, uye dhizaini yayo ichaona kuti zviri nyore sei kuronga uye kupindirana nehurongwa kugadzirisa mafambiro ebasa, kuseta maparamita ekuita, uye kuita sarudzo dzekubata data. Iyo ine zvakare yakanangana neiyo yakawanda sei kudzidziswa kunodiwa kuti ushande sisitimu nechivimbo. Kunze kwekunge uine software tekinoroji mumba, isina kugadzirwa software, kunyangwe ine simba rakadii, inogona kukusiya uchitsamira kune mutengesi kana nyanzvi yekunze kugadzira mapuroteni akarongedzerwa, matambudziko ekugadzirisa, uye kuita kunyangwe yakapusa hurongwa shanduko. MumaLab akawanda, system operator haisi nyanzvi yekuronga, uye zvikwata zvakawanda zveIT hazvipindire zvakanangana nesoftware yekudzora chiridzwa. Nekuda kweizvozvo, ungangomirira kuti vapi vezano vekunze vawanikwe, zvichikanganisa zvakanyanya kubereka uye kuisa nguva yeprojekiti panjodzi.
Pfungwa dzekufunga
Mibvunzo yakakosha yekubvunza kana uchiongorora mvura inobata system software inosanganisira:
- Vafambisi vanogona here kupindirana neiyo touchscreen yekushanda kwemazuva ese?
- Mutengesi ane raibhurari yemaprotocol aripo here kurerutsa hurongwa?
- Ndeapi maitiro ekubatanidza software kune yechitatu-bato zvishandiso?
- Ndeipi chiyero cheraibhurari yemutyairi wemidziyo inopihwa nemutengesi?
- Mutengesi ane ruzivo neLIMS interfacing?
- Ungave wakasununguka kuronga iyo system iwe pachako?
- Zviri nyore sei kune vashandisi kumisikidza kumhanya kwavo pasina hunyanzvi hwekugadzira?
- Ndeapi maficha-akadai seanogona graphical kurodha madhairekitori-aunoda, uye anowanikwa here?
- Zviri nyore here kugadzirisa zvakare software kana sisitimu yadzokororwa?
- Mutengesi anogona kubatsira here kuona cybersecurity?
Sample traceability
Yakazara sampuli traceability inogona kuve yakakosha pakuteerana nemhando zviyero uye nhungamiro. Barcode kunyora, pamwe chete nesoftware yakakodzera, zvinorerutsa kuteedzera kwemasampuli uye zvinodyiwa, uye zvinogona kudzivirira kurasikirwa kwekuteedzera. Otomatiki kunyora uye yekutevera mhinduro dzinogona zvakare:
- Ratidza nzvimbo yelabware pane dhizaini uye munzvimbo dzekuchengetera
- Ita shuwa kuti mavara ebhakodhi aiswa nemazvo uye anogona kuverengwa nemazvo
- Kurumidza kuverenga bhakodhi uye maitiro ekunhonga maitiro, uye gadzirisa kusanganisa kwepakati uye LIMS.
Sarudzo yekupindira
Zvikanganiso zvinogadzirwa nyore, asi hazvisi nyore kugadzirisa nguva dzose. Mazhinji otomatiki masisitimu anoshaya "kutanga / kumisa" kana "kubvisa" mabasa, izvo zvinogona kureva kuve nekutanga chirongwa kana iwe ukapinda chimwe chinhu zvisiri izvo kana kuda kumbomira maitiro. Tsvaga yakangwara otomatiki sisitimu inogona kuona, kunzwisisa, kushuma, uye kupora kubva mukukanganisa, nekutanga / kumisa kushanda kubvumidza yakachengeteka uye nyore mushandisi kupindirana nenzvimbo yebasa yechiridzwa panguva yekumhanya.
Summary
Automated liquid kubata kunogona kubvisa mabasa akawanda anonetesa, kuvandudza zvibereko uye kusunungura nguva yakakosha yebasa rakakosha-asi chete kana iwe ukaita mhinduro dzakakodzera. Kunyatsofungisisa pfungwa dzakurukurwa munyaya ino kuchabatsira marabhoritari kusarudza nokuchenjera, zvichiita kuti awane betsero dzokushandisa mvura otomatiki uye kuita kuti upenyu huve nyore uye hunobudirira.
Nguva yekutumira: May-10-2022