Chii chinonzi Cryovials?
Cryogenic storage vialszvidiki, zvakavharwa uye cylindrical midziyo yakagadzirirwa kuchengetedza uye kuchengetedza masampuli pane yakanyanya-yakaderera tembiricha. Kunyangwe zvechinyakare mabhodhoro aya akagadzirwa kubva kugirazi, ikozvino anowanzo kugadzirwa kubva ku polypropylene kuitira nyore uye mutengo zvikonzero. Cryovials dzakanyatsogadzirwa kuti dzimire kudziya kwakadzikira se -196 ℃, uye kugarisa marudzi akasiyana-siyana emasero. Izvi zvinosiyana kubva pakuongororwa stem masero, tupukanana, masero ekutanga kusvika kumitsetse yemasero. Kupfuura izvozvo, panogonawo kunge paine zvipenyu zvidiki zvakawanda zvakachengetwa mukaticryogenic storage vials, uyewo nucleic acid nemapuroteni anoda kuchengetwa patembiricha yekuchengeta cryogenic.
Cryogenic midziyo yekuchengetera inouya nenzira dzakasiyana siyana, uye kuwana iyo chaiyo mhando inozadzisa zvese zvaunoda kunovimbisa kuti iwe unochengetedza kuvimbika kwemuenzaniso pasina kubhadhara zvakanyanya. Verenga kuburikidza nechinyorwa chedu kuti udzidze zvakawanda nezve makiyi ekutenga ekufunga kana uchisarudza iyo cryovial chaiyo yerabhoritari application yako.
Zvivakwa zveCryogenic Vial zvekufunga nezvazvo
External vs Internal Threads
Vanhu vanowanzoita sarudzo iyi zvichibva pane zvavanoda, asi chokwadi pane misiyano yakakosha yekutarisisa pakati pemhando mbiri dzeshinda.
Marabhoritari mazhinji anowanzo sarudza mabhodhoro akaiswa mukati kuti aderedze nzvimbo yekuchengetera chubhu kuti ibvumire kupinda zvirinani mumabhokisi efriji. Pasinei neizvi, iwe unogona kufunga kuti iyo yekunze yakarukwa sarudzo ndiyo iri nani sarudzo kwauri. Ivo vanoonekwa sekutakura njodzi yakaderera yekusvibiswa, nekuda kwedhizaini inoita kuti zvinyanye kuomera chero chinhu kunze kwemuenzaniso kupinda muvharo.
Mabhodhoro ane shinda yekunze anowanzo kufarirwa ma genomic application, asi chero sarudzo inoonekwa seyakakodzera biobanking uye mamwe maapplication epamusoro ekushandisa.
Chinhu chekupedzisira chekufunga nezve tambo - kana rabhoritari yako ichishandisa otomatiki, ungangoda kufunga kuti ndeipi shinda inogona kushandiswa neiyo grippers.
Kuchengeta Vhoriyamu
Mabhodhoro eCryogenic anowanikwa muhukuru hwakasiyana-siyana kuti avhare zvidikanwi zvakawanda, asi kazhinji anosiyana pakati pehuwandu hwe1 mL ne5 mL.
Chinokosha ndechekuti uve nechokwadi chekuti cryovial yako haina kuzara uye kuti pane imwe nzvimbo inowanikwa, kana sampu ichizvimba ichitonhora. Mukuita, izvi zvinoreva kuti marabhoritari anosarudza 1 mL mabhodhoro paanenge achichengeta sampuli dze0.5 mL dzemasero akarembera mu cryoprotectant, uye 2.0 mL mabhodhoro e1.0 mL yesampuli. Rimwe zano rekuti usazadze mabhodhoro ako nderekuita kuti ushandise makiribhori ane mamakisi akapedza kudzidza, izvo zvinova nechokwadi chekudzivirira chero kuzvimba kunogona kukonzera kutsemuka kana kubuda.
Screw Cap vs Flip Pamusoro
Mhando yepamusoro yaunosarudza inotsamira zvakanyanya pakuti iwe uchange uchishandisa liquid phase nitrogen kana kuti kwete. Kana uri, saka iwe uchada screw capped cryovials. Izvi zvinovimbisa kuti hazvigone kuvhurika netsaona nekuda kwekubata zvisina kunaka kana shanduko yekushisa. Pamusoro pezvo, screw caps inobvumira kudzoreredza nyore kubva kumabhokisi e-cryogenic uye kuchengetedza kwakanyanya.
Nekudaro, kana usiri kushandisa mvura nhanho nitrogen uye uchida yepamusoro iri nyore kuvhura, saka flip top ndiyo iri nani sarudzo. Izvi zvinokusevha iwe nguva yakawanda sezvo zviri nyore kuvhura, izvo zvinoita kuti zvive zvakakosha mumabasa epamusoro ekufambisa uye ayo anoshandisa batch maitiro.
Chisimbiso Chengetedzo
Nzira yakanakisa yekuita chisimbiso chakachengeteka kuve nechokwadi chekuti cryovial cap uye bhodhoro zvese zvakavakwa kubva kune imwechete chinhu. Izvi zvichava nechokwadi chekuti vanodzikira uye kukura pamwe chete. Kana ivo vakagadzirwa vachishandisa zvinhu zvakasiyana, vanozodzikira uye kuwedzera pamitengo yakasiyana sezvo tembiricha inochinja, mapeji anotungamira uye anogona kuvuza uye kusvibiswa kunozotevera.
Mamwe makambani anopa mawachi maviri uye flange kune yepamusoro nhanho yekuchengetedza sampuli pane ekunze shinda cryovials. O-Ring cryovials inoonekwa seyakanyanya kuvimbika kune yemukati shinda cryovials.
Girazi vs Plastiki
Nekuchengetedza uye nyore, marabhoritari mazhinji ikozvino anoshandisa plastiki, kazhinji polypropylene, panzvimbo yekupisa-inovharika girazi ampules. Girazi ma ampoles ave kunzi isarudzo yechinyakare sezvo panguva yekuvharisa kusingaonekwe pinhole inodonha inogona kukura, iyo kana yanyungudutswa mushure mekuchengetwa mune yemvura nitrogen inogona kuita kuti iputika. Iwo zvakare haana kukodzera kune azvino maitiro ekurevera, inova kiyi yekuona sampuli yekuteedzera.
Self Standing vs Rounded Bottoms
Cryogenic mabhodhoro anowanikwa ese anozvimiririra ane nyeredzi-akaita mabhoti, kana seakatenderedzwa pasi. Kana iwe uchida kuisa mabhodhoro ako pamusoro saka ita shuwa yekusarudza yekuzvimiririra
Traceability uye Sample Tracking
Iyi nzvimbo yekuchengetedza cryogenic inowanzo furatirwa asi sampuli yekutevera uye kuteedzera chinhu chakakosha kufunga. Cryogenic samples inogona kuchengetwa kwemakore mazhinji, pamusoro penguva iyo vashandi vanogona kuchinja uye vasina marekodhi akachengetedzwa zvakanaka vanogona kusazivikanwa.
Ita shuwa yekusarudza mabhodhoro anoita kuti sampuli yekuzivikanwa kuve nyore sezvinobvira. Zvinhu zvaunofanira kutarisa zvinosanganisira:
Nzvimbo dzakakura dzekunyora dzekunyora zvakakwana kuitira kuti marekodhi awanikwe kana vial iri panzvimbo isiriyo - kazhinji chitupa chiziviso, zuva rakaomeswa nechando, uye mavambo emunhu ane mhosva akakwana.
Barcodes kubatsira sampuli manejimendi uye yekutevera masisitimu
Zvivharo zvemavara
Chiziviso cheramangwana - ekupedzisira-kutonhora-inodzivirira machipisi ari kugadzirwa ayo, kana akaiswa mukati meiyo cryovials, anogona kuchengetedza yakadzama nhoroondo yekupisa pamwe neruzivo rwakadzama rwebatch, mhedzisiro yebvunzo uye mamwe akakodzera emhando zvinyorwa.
Pamusoro pekupa kufunga kune akasiyana madhizaini aripo, imwe pfungwa inodawo kupihwa kune tekinoroji maitiro ekuchengeta cryovials mune yemvura nitrogen.
Storage Temperature
Kune akati wandei ekuchengetedza nzira dzekuchengetera cryogenic yemasampuli, imwe neimwe inoshanda pane imwe tembiricha. Sarudzo uye tembiricha yavanoshanda pairi inosanganisira:
Liquid chikamu LN2: chengetedza tembiricha ye -196 ℃
Vapor phase LN2: vanokwanisa kushanda patembiricha yakatarwa pakati -135°C uye -190°C zvichienderana nemuenzaniso.
Nitrogen mhute mafiriza: -20°C kusvika -150°C
Rudzi rwemasero ari kuchengetwa uye nzira yekuchengetera inosarudzwa nemuongorori ndiyo inozoona kuti ndeipi pazvitatu zviripo sarudzo inoshandisa laboratory yako.
Nekudaro, nekuda kwekudzikira kwakanyanya kunoshandiswa kwete ese machubhu kana magadzirirwo achave akakodzera kana akachengeteka. Zvishandiso zvinogona kuita brittle zvakanyanya pakudziya kwakadzikira, kushandisa vial isingakodzeri kushandiswa patembiricha yaunosarudza inogona kuita kuti ngarava ipwanye kana kutsemuka panguva yekuchengetedza kana kunyungudika.
Nyatsotarisa mazano evagadziri pakushandiswa kwakakodzera sezvo mamwe mabhodhoro e cryogenic akakodzera kutonhora kusvika -175 ° C, mamwe -150 ° C mamwe 80 ° C chete.
Izvo zvakakoshawo kuziva kuti vagadziri vazhinji vanotaura kuti yavo cryogenic vials haina kukodzera kunyudzwa muchikamu chemvura. Kana mabhodhoro aya akachengetwa muchikamu chemvura paanenge achidzokera kune tembiricha yekamuri aya mabhodhoro kana zvisimbiso zvawo zvinogona kupwanyika nekuda kwekukurumidza kuvakwa kwekumanikidza kunokonzerwa nekudonha kudiki.
Kana maseru achizochengetwa muchikamu chemvura chenitrogen yemvura, funga kuchengetedza maseru mumidziyo ye cryogenic yakakodzera kupisa-yakavharwa mu cryoflex tubing kana kuchengeta maseru mumagirazi ampules akavharwa zvine mutsindo.
Nguva yekutumira: Nov-25-2022