96-tsime rakadzika tsime ndiro (Deep Well Plate) imhando yendiro ine matsime akawanda inowanzoshandiswa mumarabhoritari. Iyo ine yakadzika gomba dhizaini uye inowanzo shandiswa pakuyedza inoda mavhoriyamu akakura emasampuli kana reagents. Aya anotevera ndiwo mamwe emhando huru dzekushandisa uye nzira dzekushandisa dze96-tsime rakadzika tsime ndiro:
Application range:
High-throughput screening: Mukuedza kwakadai sekuongorora zvinodhaka uye kuongororwa kweraibhurari, 96-tsime rakadzika tsime mahwendefa anogona kugarisa mamwe masampuli uye kunatsiridza hunyanzvi hwekuyedza.
Cell tsika: Inokodzera kuyedza tsika dzemasero dzinoda huwandu hukuru hwetsika yepakati, kunyanya tsika yemaseru anoomerera.
Enzyme-yakabatana immunosorbent assay (ELISA): Inoshandiswa mune ELISA zviedzo zvinoda huwandu hukuru hwekuita system.
Molecular biology kuyedza: Zvakadai sePCR maitiro, DNA / RNA kutorwa, electrophoresis sampuli kugadzirira, nezvimwe.
Mataurirwo eprotein uye kucheneswa: Inoshandiswa mukuyedza neprotein yakakura kutaura kana inoda huwandu hukuru hwebuffer.
Yenguva refu yekuchengetedza sampuli: Nekuda kwegomba rakakura kudzika, shanduko yevhoriyamu yemuenzaniso panguva yechando inogona kuderedzwa, iyo yakakodzera kuchengetedza kwenguva refu.
Nzira yekushandisa:
Kugadzirira kwemuenzaniso: Zvinoenderana nezvinodiwa zvekuyedzwa, nyatsoyera huwandu hwakakodzera hwesample kana reagent uye woiwedzera kune tsime remvura yakadzika ndiro.
Kuisa chisimbiso: Shandisa firimu rekuvhara rakakodzera kana gasket kuvhara ndiro yetsime kudzivirira kubuda kwemuenzaniso kana kusvibiswa.
Kusanganisa: Zvinyoro zunza kana kushandisa multichannel pipette kusanganisa sampuli kuti ive nechokwadi chekuti sampuli yakanyatsobatana neiyo reagent.
Incubation: Isa iyo yakadzika-mutsime ndiro mubhokisi rekushisa rinogara riripo kana imwe nharaunda yakakodzera yekuisa zvinoenderana nezvinodiwa zvekuedza.
Kuverenga dhata: Shandisa zviridzwa zvakaita semamicroplate kuverenga uye fluorescence maikorosikopu kuti uverenge zviyedzo zvabuda.
Kuchenesa nekuuraya utachiona: Mushure mekuedza, shandisa mishonga yakakodzera kuchenesa ndiro yakadzika-mutsime uye kuiuraya.
Kuchengeta: Ndiro yakadzika-mutsime inofanira kuchengetedzwa zvakanaka mushure mekucheneswa uye nekuuraya utachiona kudzivirira kusvibiswa.
Paunenge uchishandisa 96-tsime yakadzika-tsime mahwendefa, zvinotevera pfungwa dzinofanirawo kucherechedzwa:
Maitiro ekushanda: Tevedzera aseptic mashandiro ekuita kuti udzivise kusvibiswa kwemuenzaniso.
Kurongeka: Shandisa multichannel pipette kana otomatiki yemvura inobata sisitimu yekuvandudza iko iko kushanda.
Zvakajeka kumaka: Ita shuwa kuti tsime rega rega remugodhi rakanyorwa zvakajeka kuti rizivikanwe uye kurekodha zviri nyore.
96-tsime rakadzika-tsimemahwendefa chinhu chakakosha chekuedza kwepamusoro-soro murabhoritari. Kushandisa kwakakodzera kunogona kuvandudza zvakanyanya kushanda uye kurongeka kwekuyedza.
Nguva yekutumira: Aug-13-2024