Iyo micropipette ndiyo inonyanya kushandiswa murabhoritari. Iwo anoshandiswa nemasaenzi munzvimbo dzakasiyana siyana dzinosanganisira academia, chipatara uye forensics labs pamwe nekugadzirwa kwezvinodhaka uye vaccine kuendesa chaiyo, shoma shoma yemvura.
Nepo zvichigona kutsamwisa uye kushungurudza kuona mabhuru emhepo mupipette inoraswa kana isina kuoneka kana kufuratirwa inogona kuve nemhedzisiro yakakura pakuvimbika uye kuberekana kwemhedzisiro.
Nhau dzakanaka ndedzekuti kune mamwe matanho akareruka aunogona kutora kudzivirira mabhuru emhepo, uye kugadzirisa lab kushanda zvakanaka, kugutsikana kwemushandisi pamwe nekurongeka uye nemazvo emhedzisiro.
Pazasi, isu tinoongorora mhedzisiro yekuwana bhobho remhepo mune yako pipette tipi uye zvaunofanira kuita zvinotevera.
Mhedzisiro yeBubbles muPipette Tip
Kunyangwe iwe ukashandisa iyo yakanyanya kurongeka, pamusoro peiyo renji, yakanyatsochengetedzwa, yakashumirwa uye yakagadziriswa pipettes kuvimbika kwemhedzisiro yako kunogona kukanganiswa nezvikanganiso zverabhu. Kana mabubbles apinda mutipinogona kuva nemigumisiro yakawanda.
● Kana mushandisi aona bubble remhepo anofanira kupedza nguva achipa mvura inofefetera nenzira kwayo, bvisa chipeto uye wotanga hurongwa zvakare.
● Mafuro emhepo asingaonekwi anogona kukonzera kuderedzwa kwevhoriyamu, nokudaro kushandura kusanganiswa kwemaitiro anosanganisa zvinotungamira mukukundikana kuedza uye mhedzisiro inopokana kana isingavimbike.
Mhedzisiro iyi inogona kuve nemhedzisiro yakati wandei (1).
● Kudzikira kweLab Kubudirira - Miedzo uye maassays achafanirwa kudzokororwa, zvichiunza mutengo wevashandi uye zvinhu, izvo zvinogona kunge zvakanyanya.
● Migumisiro Inobvunzwa kana Yakashata - Kana migumisiro isina kururama ikasunungurwa panogona kuva nemigumisiro yakakomba inosanganisira kusaziva uye migumisiro yakaipa yemurwere.
● Kudzorwa Kwezvinyorwa Kubva Muzvinyorwa - Kana vezera rako vakatadza kudzokorora zvawawana nekuda kwemafuru emhepo zvichikonzera kuti mapepa emhedzisiro anogona kubviswa.
Maitiro Akanyanya Kudzivirira Mhepo Mabhuru
Muzviitiko zvakawanda mabhuru emhepo mumapipette mazano anokonzerwa nekukanganisa kwevashandi. Unyanzvi husina kunaka nekuda kwekusakwana kudzidziswa kana kuneta kazhinji ndiro dambudziko guru.
Pipetting ibasa rine hunyanzvi iro rinoda 110% kutariswa, kudzidziswa kwakaringana uye kudzidzira kuti uwane mhedzisiro uye yakarurama.
Nepo paine zvinhu zvakati wandei zvaunogona kuita kuderedza zvikanganiso zvepombi, pazasi takaratidza mamwe maitiro akanakisa anogona kushandiswa kudzivirira mabhuru emhepo mukati.pipette mazano.
Kuvandudza Mushandisi Tekiniki
Pipette Zvishoma nezvishoma
Kana iyo plunger ikaburitswa nekukurumidza kana ichishuvira, mabhuru emhepo anogona kuunzwa mumuromo. Izvi zvinogona kunyanya kunetsa kana uchiendesa viscous zvinwiwa. Mhedzisiro yakafanana inogona kuitika kana plunger ikaburitswa nekukurumidza mushure mekupa.
Kuti udzivise mabhubhu emhepo paunenge uchishuvira, chenjerera kushandisa piston yemapipeti echinyorwa nenzira yakatsetseka uye yenguva dzose, uchishandisa simba risingachinji.
Shandisa Iyo Yakarurama Kunyudza Kudzika
Kutadza kunyudza iyo pipette tip yakadzika pasi pe meniscus yemvura inochengeterwa inogona kuguma nekushuvira kwemhepo uye nokudaro kuumbwa kwebubble.
Nekudaro, kunyudza iyo tipi yakadzika zvakanyanya kunogona kushuvira mvura yakawanda nekuda kwekuwedzera kudzvanywa kana madonhwe anogona kuitika kunze kwetipi saka zvakakosha kunyudza.pipette tipkusvika pakudzika chaiko.
Iyo yakakurudzirwa kudzika inosiyana pakati pepipette saizi, mhando uye kugadzira. Ipo kurudziro yevagadziri inofanirwa kuteverwa heino gwara rakapihwa neNational Physical Laboratory.
Nhungamiro Kudzama Kwezano Kunyudzwa
Pipette Vhoriyamu (µl) & Kunyudza Kudzika (mm)
- 1 – 100: 2 – 3
- 100 - 1,000: 2 - 4
- 1,000 - 5,000: 2 - 5
Pre-WetPipette Mazano
Kana pombi mavhoriyamu makuru kupfuura 10µlpipette mazanoanowanzo pre-kunyoroveswa nekuazadza kakawanda nemvura iri kubudiswa uye kuibvisa kuti iparare kuti ivandudze chokwadi.
Kutadza kufanonyorova kunogona kukonzera mabhuru emhepo, kunyanya kana uchishandisa viscous kana hydrophobic zvinwiwa. Kuti udzivise mabhuru emhepo chengetedza matipi ekare-akanyorova kana pombi yevhoriyamu inopfuura 10µl.
Shandisa Reverse Pipetting Techniques Kana Zvakakodzera
Viscous Substances: Dambudziko rakajairika kana pipetting viscous zvinhu zvakaita seprotein kana nucleic acids mhinduro, glycerol uye Tween 20/40/60/80 ndiko kugaro kugadzirwa kwemabhuru kana nzira yekumberi yekubheta ichishandiswa.
Kurovera pombi zvishoma nezvishoma, kushandisa iyo reverse pipetting nzira inoderedza njodzi yekuumbwa kwebubble kana uchiendesa viscous mhinduro.
ELISA Technique
Reverse pipetting inokurudzirwawo paunenge uchipinza mavhoriyamu madiki mukati96 zvakanaka micro test mahwendefakune ELISA maitiro. Kana mabhuru emhepo achidhonzwa mupipette kana kuisirwa mumatsime kana uchiwedzera reagents zvinogona kukanganisa optical density values uye mhedzisiro. Reverse pipetting inokurudzirwa kuderedza kana kubvisa nyaya iyi.
Shandisa Ergonomic Pipettes
Mapipeti echinyakare asina kugadzirwa aine ergonomics mupfungwa anoda simba rakawanda remuviri, unoneta uye hunyanzvi hwako hwekupopa hunova husina maturo uye hurombo. Zvikanganiso zvataurwa pamusoro apa sekukurumidza kuburitsa plunger zvinogona kuitika kakawanda.
Nekudyara mune imwe ergonomic mhinduro iwe unozokwanisa kuchengetedza yakanakisa hunyanzvi uye kudzivirira mabhuru emhepo kuumbwa nekuda kwemaitiro asina kunaka.
Tora Nguva Yekudzidzisa Vashandi
Kugara uchidzidzira uye kuongorora vashandi mumapipetting matekiniki vanogona kuve nechokwadi chekuti chikanganiso chemushandisi uye kuumbwa kwemhepo bubble kwakaderedzwa.
Funga Zvimwe Zvigadziriswa Zvigadziriswa
Sezvakataurwa pamusoro apa akawanda mabhuru emhepo anokonzerwa nemushandisi. Zvinogona kuita kudzikisa kukanganisa kwevashandisi uye nyaradzo nekushandisa magetsi pipettes kana inoshanduka mvura inobata chikuva sengeAgilent Bravo Liquid Inobata Robot.
Shandisa Hunhu HwakanakaPipette Mazano
Micropipettes inowanzotengwa nekuchenjerera, asi kazhinji pfungwa shoma inopiwa kune kunaka kwepipette tip inoraswa. Nekuda kwepesvedzero ine matipi pamhedzisiro yepombi, iyo standardISO 8655 inoda imwe calibration kana mapaipi uye matipi kubva kune vakasiyana vagadziri vakashandiswa.
Izvi zvinogona kunge zviri nekuti matipi mazhinji akachipa anogona kutaridzika zvakanaka pakutanga asi kana ukanyatsoadzidza anogona kunge aine kupenya, maprotrusions, makwara, uye mabhuru emhepo, kana kukotama kana kuve netsvina.
Kutenga matipi akanaka emhando yepamusoro akagadzirwa nepamusoro-giredhi polypropylene kunogona kuderedza kuitika kwemabhuru emhepo.
Kupedzisa
Kuwana mabhuru emhepo mune yako pipette tip kune kukanganisa kwekushanda kwelabhu pamwe nekusarongeka uye kusarongeka kwemhedzisiro. Takaona zvinhu zvakati wandei zvaunogona kuita kudzivirira mabhuru emhepo kupinda mukatipipette tip.
Zvisinei, kana murombo unhupipette mazanovari kukonzera mabhuru emhepo kupinda mupipette tip yako, iwe uchafadzwa kuziva kuti yedu yepasirese yakwanapipette mazanoanogadzirwa kune epamusoro zviyero uye anogadzirwa ne premium-giredhi pure polypropylene.
Suzhou Ace Biomedical kambanigadzira zvemhando yepamusoro gumi, 20, 50, 100, 200, 300,1000 uye 1250 µL mavhoriyamu epasirese pipette mazano, 96 matipi/rack. Kugara kwakasarudzika - ese eACE tip racks anomira kune izvo zvinodikanwa zvekushandisa nemapipettor akawanda. Sterile, Sefa, RNase-/DNase-isina, uye nonpyrogenic.
Kugamuchirwa kuti utibvunze kuti uwane rumwe ruzivo.
Nguva yekutumira: Zvita-29-2022