Nyanzvi dzemuLabhoreti dzinogona kupedza maawa zuva rega rega dzakabata micropipette, uye kuvandudza kukwenenzverwa kwepombi uye kuona mhedzisiro inovimbika kazhinji kunonetsa.Kusarudza iyo micropipette yakakodzera kune chero yakapihwa application chinhu chakakosha pakubudirira kwebasa remurabhoritari; haisi kungovimbisa kushanda kwekuedza chero kupi zvako, asiwo kunowedzera kushanda.Kunzwisisa zvinodikanwa zvepipetting workflow inoita kuti vashandisi vasarudze pipettes yakarurama uye inodzokororwa, asi pane zvimwe zvinhu zvakawanda zvinofanirwa kutariswa kuvandudza mhedzisiro yepipetting uye kuvimbisa kubudirira kwe experiments.
Mukutaura, zvinwiwa zvinowira muzvikamu zvitatu zvikuru: aqueous, viscous, uye volatile.Zvinwiwa zvakawanda zvinokonzerwa nemvura, zvichiita kuti air displacement pipettes ive sarudzo yekutanga kune vakawanda.Kunyange zvazvo mvura zhinji inoshanda zvakanaka nemhando iyi yepipette, volumetric pipettes inofanira kusarudzwa apo kushanda nezvinwiwa zvakasvibirira zvakanyanya kana zvinoputika.Misiyano pakati pemhando idzi dzepipette inoratidzwa muMufananidzo 1. Zvakakoshawo kushandisa nzira yakarurama yepipetting - pasinei nemhando yemvura - kuti zvive nemigumisiro yakanaka.
Izvo zvikamu zviviri zvakakosha zvakanyanya zvinokanganisa mhedzisiro yepipetting ndeyechokwadi uye chakarurama (Mufananidzo 2) .Kuti uwane huwandu hwepipetting yakarurama, yakarurama, uye kuvimbika, mitemo yakawanda inofanira kuchengetwa mupfungwa. iyo inokwanisa kubata vhoriyamu inodiwa yekufambisa.Izvi zvakakosha sezvo huroyi huchidzikira sezvo vhoriyamu yakaseti inosvika kuvhoriyamu diki yepipette.Semuyenzaniso, ukaburitsa 50 µl ne5,000 µl pipette, mibairo inogona kunge isina kunaka.Zvibereko zviri nani zvinogona kuva yakawanikwa ine 300 µl pipettes, nepo 50 µl pipettes inopa mibairo yakanaka. Pamusoro pezvo, vhoriyamu yakaiswa pamapipette echinyakare emaoko inogona kushanduka panguva yepipetting nekuda kwekutenderera netsaona kweplunger.Ndosaka vamwe vagadziri vepipette vakagadzira magadzirirwo ekuvhara vhoriyamu kudzivirira. kusandura kusingafungidzirwe uku uchipomba kuti uwedzere kuve nechokwadi.Calibration chimwe chinhu chakakosha chinobatsira kuvimbisa mibairo yakavimbika nekuratidza kurongeka uye kurongeka kwepipette.Iyi nzira inofanira kuva nyore kumushandisi; semuenzaniso, mamwe mapipette emagetsi anogona kuseta zviyeuchidzo zvekugadzirisa, kana kuchengetedza nhoroondo yekuenzanisa.Haisi mapipette chete ekufunga.Kana chipipeti chepipette chikaita kusununguka, kudonha, kana kudonha, chinogona kukonzera matambudziko akasiyana.Iri dambudziko rinowanzoitika murabhoritari. kazhinji inokonzerwa nekushandiswa kwemazano epipette ezvechinangwa, ayo anowanzoda "kutapa."Iyi nzira inotambanudza mupendero wepipette tip uye inogona kuita kuti muromo udonhe kana kuti uise panzvimbo, kana kutokonzera kuti muromo udonhe kubva papipette zvachose. .Kusarudza micropipette yepamusoro yakagadzirwa nemazano chaiwo inoita kuti pave nekubatana kwakachengeteka, kupa huwandu hwepamusoro hwekuvimbika uye migumisiro iri nani.Kuwedzera, chimwe chinhu chiri nyore sepipettes yemavara-coding uye mazano anogonawo kubatsira vashandisi kuti vaone kuti mazano akakodzera anosarudzwa. mapaipi avo.
Munzvimbo yepamusoro-soro, zvakakosha kuti zvive zvakanaka sezvinobvira paunenge uchichengetedza kuvimbika uye kuwirirana kwepipetting process.Kune nzira dzakawanda dzekuvandudza kushandiswa kwepipetting, kusanganisira kushandiswa kwe-multichannel uye / kana electronic pipettes.Izvi zvishandiso zvakasiyana-siyana. kazhinji inopa nzira dzakasiyana-siyana dzepipetting-zvakadai sereverse pipetting, variable dispensing, programmed serial dilutions, nezvimwe-kurerutsa maitiro.Semuyenzaniso, maitiro akadai sekudzokororwa kudhindwa kwakanakira kugovera akawanda aliquots evhoriyamu imwechete pasina kuzadzazve muromo. Kushandisa mapipette e-single-channel kuendesa sampuli pakati pemhando dzakasiyana dze labware kunogona kukurumidza kunetesa uye kukanganisa-kukanganisa.Multichannel pipettes inobvumira kutamisa masampuli akawanda panguva imwe chete mukubwaira kweziso. zvikanganiso uye kudzokorora kukuvara kuomarara (RSI) .Mamwe pipettes anotova nekwanisi yekusiyana matipi spacing panguva yepipetting, achibvumira kuchinjika kwakafanana kwemasampuli akawanda pakati pehukuru hwakasiyana hwelabware uye mafomati, kuchengetedza maawa enguva (Figure 3).
Nyanzvi dze laboratory dzinowanzopedza maawa pazuva pipetting.Izvi zvinogona kukonzera kusagadzikana uye, mumatambudziko akaoma, kunyange kukuvara kwemaoko kana ruoko.Zano rakanakisisa rekudzivisa izvi zvinogona kuitika nderokuderedza nguva yaunobata pipette kusvika kunguva pfupi. .Kuwedzera kune izvi, vashandisi vanofanira kusarudza micropipette yakareruka uye yakanyatsogadzikana ine uwandu huri pakati pekugadzirisa zviri nani.Pipette inofanira kukwana zvakanaka mumaoko evashandisi vekuruboshwe uye kurudyi, kuva nekugadzirisa kwakanaka, uye kugadzirisa. iyo vhoriyamu yakasununguka uye nekukurumidza sezvinobvira kudzivirira kufamba kusingakoshi.Uyewo, mazano akakosha, sezvo kurodha matipi uye ejection kazhinji kunoda simba rakawanda kupfuura pipetting uye kune njodzi inogona kuitika yekukuvara, kunyanya mumagadzirirwo epamusoro.Pipette mazano anofanira kukurumidza munzvimbo ine simba shoma, ipa chinongedzo chakachengeteka, uye zvive nyore kubudisa.
Paunenge uchisarudza iyo micropipette yakakodzera yekushandisa kwako, zvakakosha kuti utarise pane ese mafambisirwo ebasa rako.Nekutarisa pipette, maitiro ayo, rudzi uye huwandu hwemvura iri kuiswa pombi, uye matipi anoshandiswa, vesainzi vanogona kuvimbisa kwakaringana, kwakaringana uye kwakavimbika. mhedzisiro paunenge uchichengetedza kubereka uye kuderedza njodzi yekukuvara.
Mune ino edition, kudzoreredzwa kweanokosha analytes kunoongororwa neHPLC-MS vachishandisa yakasanganiswa-modhi yakasimba cation exchange SPE microplates. Mabhenefiti eSEC-MALLS mune biopharmaceutical application…
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Nguva yekutumira: Jun-10-2022