Nyanzvi dzemuLabhoreti dzinogona kupedza maawa zuva rega rega dzakabata micropipette, uye kuvandudza kukwenenzverwa kwepombi uye kuona mhedzisiro inovimbika kazhinji kunonetsa.Kusarudza iyo micropipette yakakodzera kune chero yakapihwa application chinhu chakakosha pakubudirira kwebasa remurabhoritari; haisi kungovimbisa kushanda kwekuedza chero kupi zvako, asiwo kunowedzera kushanda.Kunzwisisa zvinodikanwa zvepipetting workflow inoita kuti vashandisi vasarudze pipettes yakarurama uye inodzokororwa, asi pane zvimwe zvinhu zvakawanda zvinofanirwa kutariswa kuvandudza mhedzisiro yepipetting uye kuvimbisa kubudirira kwe experiments.
Mukutaura, zvinwiwa zvinowira muzvikamu zvitatu zvikuru: aqueous, viscous, uye volatile.Zvinwiwa zvakawanda zvinokonzerwa nemvura, zvichiita kuti air displacement pipettes ive sarudzo yekutanga kune vakawanda.Kunyange zvazvo mvura zhinji inoshanda zvakanaka nemhando iyi yepipette, volumetric pipettes inofanira kusarudzwa apo kushanda nezvinwiwa zvine viscous zvakanyanya kana zvinovhuvhuta.Misiyano pakati pemhando idzi dzepipette inoratidzwa muMufananidzo 1. Zvakakoshawo kushandisa nzira yakarurama yepipetting - pasinei nemvura. mhando - kune mhedzisiro yakanaka.
Izvo zvikamu zviviri zvakakosha zvakanyanya zvinokanganisa mhedzisiro yepipetting ndeyechokwadi uye chakarurama (Mufananidzo 2) .Kuti uwane huwandu hwepipetting yakarurama, yakarurama, uye kuvimbika, mitemo yakawanda inofanira kuchengetwa mupfungwa. iyo inokwanisa kubata vhoriyamu inodiwa yekufambisa.Izvi zvakakosha sezvo kurongeka kunoderera sezvo vhoriyamu yakatarwa inoswedera kudiki vhoriyamu yepipette.Semuyenzaniso, kana ukaburitsa 50 µl ine 5,000 µl pipette, mibairo inogona kushata. Mibairo iri nani inogona kuwanikwa ne300 µl pipettes, ukuwo 50 µl pipettes ichipa mibairo yakanaka. Pamusoro pezvo, vhoriyamu yakasetwa pamapipeti echinyakare anogona kuchinja panguva yekuridza pombi nekuda kwekutenderera netsaona kwe Ndokusaka vamwe vagadziri vemapipete vakagadzira kukiya vhoriyamu gadziriso madhizaini kudzivirira kushanduka kusingafungidzirwe paipiping. kuti uwedzere kuva nechokwadi chekururama.Calibration chimwe chinhu chakakosha chinobatsira kuvimbisa mhedzisiro yakavimbika nekuratidza kurongeka uye kurongeka kwepipette.Iyi nzira inofanira kuva nyore kumushandisi; semuenzaniso, mamwe mapipette emagetsi anogona kuseta zviyeuchidzo zvekugadzirisa, kana kuchengetedza nhoroondo yekuenzanisa.Haisi mapipette chete ekufunga.Kana chipipeti chepipette chikaita kusununguka, kudonha, kana kudonha, chinogona kukonzera matambudziko akasiyana.Iri dambudziko rinowanzoitika murabhoritari. kazhinji inokonzereswa nekushandiswa kwemapipette ezvechinangwa matipi, ayo anowanzoda "kutapa."Iyi nzira inotambanudza mupendero wepipette tip uye inogona kuita kuti muromo udonhe kana misplace, kana kutoita kuti muromo udonhe kubva papipette zvachose.Kusarudza micropipette yepamusoro yakagadzirwa nemazano chaiwo inoita kuti pave nekubatana kwakachengeteka, kupa hutano hwepamusoro hwekuvimbika nemigumisiro iri nani.Kuwedzera, chimwe chinhu chiri nyore sepipettes yemavara-coding uye matipi anogona zvakare kubatsira vashandisi kuona kuti matipi akakodzera anosarudzwa kune avo pipette.
Munzvimbo yepamusoro-soro, zvakakosha kuti zvive zvakanaka sezvinobvira paunenge uchichengetedza kuvimbika uye kuwirirana kwepipetting process.Kune nzira dzakawanda dzekuvandudza kushandiswa kwepipetting, kusanganisira kushandiswa kwe-multichannel uye / kana electronic pipettes.Izvi zvishandiso zvakasiyana-siyana. kazhinji inopa nzira dzakasiyana-siyana dzepipetting-zvakadai sereverse pipetting, variable dispensing, programmed serial dilutions, nezvimwe-kurerutsa maitiro.Semuenzaniso, maitiro sekudzokororwa kugovera kwakanakira kugovera akawanda aliquots evhoriyamu imwechete pasina kuzadzazve muromo.Kushandisa single-channel pipette kuendesa sampuli pakati pemhando dzakasiyana dzelabware kunogona kukurumidza kuve kunetesa uye kukanganisa-kukanganisa.Multichannel pipettes inobvumira kutamisa masampuli akawanda panguva imwe chete. mukubwaira kweziso.Izvi hazvisi izvo chete zvinowedzera kushanda zvakanaka, zvinobatsirawo kudzivirira zvikanganiso zvepipetting uye kudzokorora kukuvara kwekukuvara (RSI) .Vamwe mapipette anotokwanisa kusiyanisa matip spacing panguva yepipetting, achibvumira kufambiswa kwakafanana kwemasampuli akawanda pakati peakasiyana labware saizi uye mafomati, kuchengetedza maawa enguva (Mufananidzo 3).
Nyanzvi dze laboratory dzinowanzopedza maawa pazuva pipetting.Izvi zvinogona kukonzera kusagadzikana uye, mumatambudziko akaoma, kunyange kukuvara kwemaoko kana ruoko.Zano rakanakisisa rekudzivisa izvi zvinogona kuitika nderokuderedza nguva yaunobata pipette kusvika kunguva pfupi. .Kuwedzera kune izvi, vashandisi vanofanira kusarudza micropipette yakareruka uye yakanyatsogadzikana ine uwandu huri pakati pekugadzirisa zviri nani.Pipette inofanira kukwana zvakanaka mumaoko evashandisi vekuruboshwe uye kurudyi, vane zvakanaka. kubata dhizaini, uye gadzirisa vhoriyamu zvakasununguka uye nekukurumidza sezvinobvira kuti udzivise kufamba kusingakoshi.Uyewo, mazano akakosha, sezvo matipi ekurodha uye ejection kazhinji inoda simba rakawanda kupfuura pipetting uye kune njodzi inogona kuitika yekukuvara, kunyanya mu-high-throughput settings. .Mazano ePipette anofanira kupinda munzvimbo nesimba shoma, kupa kubatana kwakachengeteka, uye kuve nyore zvakaenzana kubvisa.
Paunenge uchisarudza iyo micropipette yakakodzera yekushandisa kwako, zvakakosha kuti utarise pane ese mafambisirwo ebasa rako.Nekutarisa pipette, maitiro ayo, rudzi uye huwandu hwemvura iri kuiswa pombi, uye matipi anoshandiswa, vesainzi vanogona kuvimbisa kwakaringana, kwakaringana uye kwakavimbika. mhedzisiro paunenge uchichengetedza kubereka uye kuderedza njodzi yekukuvara.
Mune ino edition, kudzoreredzwa kweanokosha analytes kunoongororwa neHPLC-MS vachishandisa yakasanganiswa-modhi yakasimba cation exchange SPE microplates. Mabhenefiti eSEC-MALLS mune biopharmaceutical application…
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Nguva yekutumira: Jun-10-2022