Polymerase chain reaction (PCR) inzira inoshandiswa zvakanyanya mumolecular biology yekukwidziridza zvimedu zveDNA. PCR inosanganisira matanho akati wandei, kusanganisira denaturation, annealing, uye kuwedzera. Kubudirira kwehunyanzvi uhu kunoenderana zvakanyanya nemhando yePCR mahwendefa uye machubhu anoshandiswa. Pane zvinhu zvakawanda zvekufunga nezvazvo paunosarudza mahwendefa ePCR nemachubhu akakodzera ekushandisa kwako.Hezvino zvimwe zvakakosha zvekufunga nezvazvo:
1. KugonaPCR mahwendefauye machubhu anouya muhukuru hwakasiyana uye kugona. Sarudzo yehukuru uye kugona kunoenderana zvakanyanya nehuwandu hweDNA inoda kukwidziridzwa mukuita kumwe chete. Semuenzaniso, kana iwe uchida kukudza diki diki reDNA, unogona kusarudza diki chubhu. Kana DNA yakawanda ichida kuwedzerwa, ndiro ine simba guru inogona kusarudzwa.
2. Material PCR mahwendefa uye machubhu anogona kugadzirwa akasiyana zvinhu zvakadai polypropylene, polycarbonate kana acrylic. Polypropylene ndiyo inonyanya kushandiswa zvinhu nekuda kwemakemikari ayo uye kudzivirira kupisa. Iyo zvakare inodhura zvishoma kana ichienzaniswa nezvimwe zvinhu. Polycarbonates uye acrylics anodhura zvakanyanya, asi ane zvirinani kujeka kwemaziso uye akanakira chaiyo-nguva PCR.
3. Thermal conductivity PCR inosanganisira akawanda ekupisa kutenderera, kunoda kukurumidza kupisa uye kutonhora kweiyo reaction musanganiswa. Naizvozvo, PCR mahwendefa uye machubhu anofanirwa kunge aine yakanaka yekupisa conductivity kuti ave nechokwadi chekudziya yunifomu uye kutonhora kwemusanganiswa wekuita. Ndiro dzine madziro akatetepa uye dzakati sandarara dzakanakira kukwidziridza kutamisa kupisa.
4. Kuenderana PCR mahwendefa uye machubhu zvinofanira kuenderana ne thermal cycler yauri kushandisa. Plates nemachubhu zvinofanirwa kushingirira tembiricha yepamusoro inodiwa pakukwidziridzwa kwezvidimbu zveDNA. Gara uchibvunza mugadziri we thermal cycler kune anokurudzirwa ndiro nemachubhu.
5. Chisimbiso Chisimbiso chakasimba chakakosha kudzivirira kusvibiswa kwekuita musanganiswa. PCR mahwendefa uye machubhu anogona kuiswa chisimbiso achishandisa nzira dzakasiyana dzakadai sezvisimbiso zvekupisa, mafirimu anonamatira kana mavharo. Kuisa chisimbiso chekupisa ndiyo nzira yakachengeteka uye inopa chipingamupinyi chakasimba kurwisa kusvibiswa.
6. Sterilization PCR mahwendefa uye machubhu anofanira kunge asina chero tsvina inogona kukanganisa maitiro. Naizvozvo, dzinofanirwa kuvharwa dzisati dzashandiswa. Zvakakosha kusarudza mahwendefa uye machubhu ari nyore kuita sterillize uye anopesana nemakemikari uye kupisa sterilization nzira.
Muchidimbu, kusarudza iyo chaiyo PCR ndiro uye machubhu kwakakosha pakubudirira kweDNA kukwidziridzwa. Sarudzo inotsamira zvakanyanya parudzi rwekushandisa, huwandu hweDNA yakakwidziridzwa, uye kuenderana neanopisa mabhasikoro.
Iyo kambani Suzhou Ace Biomedical Technology Co., Ltd. inopa huwandu hwemhando yepamusoro yePCR mahwendefa uye machubhu muhukuru hwakasiyana, hunyanzvi uye zvinhu zvinosangana nezvinodiwa nemuongorori wega wega.
Nguva yekutumira: May-17-2023