Pipette nimwe mubikoresho bisanzwe bikoreshwa mubinyabuzima, amabati, hamwe na laboratoire aho hakenewe gupimwa neza no kwimurirwa mugihe ukora ingendo, igasuzuma cyangwa amaraso. Zirahari nka:
Umuyoboro umwe cyangwa umuyoboro-mwinshi
Umubumbe uteganijwe cyangwa uhinduka
③ Imfashanyigisho cyangwa elegitoronike
Ni ubuhe bwoko bw'imiyoboro umwe?
Umuyoboro umwe-umuyoboro wemerera abakoresha kohereza aliquot imwe icyarimwe. Ibi bikunda gukoreshwa muri laboratoire hamwe na progaramu nkeya zingero, zishobora kuba abagize uruhare mubushakashatsi niterambere.
Umuyoboro umwe-umuyoboro umwe ufite umutwe umwe kugirango wishure cyangwa utange urwego rwuzuye rwamazi binyuze mubyifuzoinama. Barashobora gukoreshwa mubisabwa byinshi muri laboratoire bifite gusa progaramu nto. Ibi akenshi ni laboratoire zikora ubushakashatsi bujyanye nubushakashatsi bwerekeye isesengura, umuco wakagari, genetiki cyangwa umujinya.
Ni ubuhe bwoko bw'imiyoboro myinshi?
Umuyoboro wa benshi ukora muburyo bumwe nkumuyoboro umwe, ariko ukoresha byinshiinamaKubipima no gutanga amafaranga angana icyarimwe. Ibisanzwe bisanzwe ni imiyoboro 8 cyangwa 12 ariko 4, 6, 16 na 48 umuyoboro urahari. Imiyoboro 96 ya intenchtop irashobora no kugurwa.
Gukoresha umuyoboro-munini wa pipette, biroroshye kuzuza vuba 96-, 384-, cyangwa 1.536-nezaIsahani ya Microtiter, niyari kuba bikubiyemo ingero za porogaramu nko kongeramo ADN, Elisa (ikizamini cyo gusuzuma), ubushakashatsi bwa kinetic na molekile.
Umuyoboro umwe-Umuyoboro-Umuyoboro mwinshi
Gukora neza
Umuyoboro umwe-umuyoboro ni mwiza mugihe ukora akazi gageragejwe. Ibi ni ukubera ko ahanini bikubiyemo gukoresha imiyoboro kugiti cye, cyangwa umukino umwe wambukiranya kugirango ugabanye amaraso.
Ariko, ibi byihuse bihinduka igikoresho kidahagije mugihe winjijwe byiyongereye. Iyo hari ingero nyinshi / reagent yo kwimura, cyangwa isuzuma rinini ririmo gukoreshwaAmasahani ya microtitre 96, hari uburyo bunoze cyane bwo kwimura amazi hanyuma akoresha umuyoboro umwe. Mugukoresha umuyoboro mwinshi aho, umubare wintambwe ya pietting zigabanuka cyane.
Imbonerahamwe ikurikira irerekana umubare wintambwe ya pietting isabwa kumuyoboro umwe, 8 na 12 umuyoboro.
Umubare wintambwe ya pietting isabwa (6 reagent x96 Isahani ya Microtitre)
Umuyoboro umwe-Pipette: 576
8-Umuyoboro wa Pipette: 72
Umuyoboro wa 3: 48
Ingano ya fapetting
Itandukaniro rimwe ryingenzi hagati yumuyoboro umwe numuyoboro mwinshi nijwi kuri buri makosa ashobora kwimurwa icyarimwe. Nubwo biterwa nicyitegererezo kikoreshwa, muri rusange ntushobora kwimura amajwi menshi kumutwe kumuyoboro-wumuyoboro mwinshi.
Umubumbe w'umuyoboro umwe urashobora kwimura iri hagati ya 0.1UL na 10.000UL, aho urwego rwo mu muyoboro w'ibinyoma ruri hagati ya 0.2 na 1200ul.
Icyitegererezo
Amateka, imiyoboro myinshi yo mu mateka ntiyari unfite kandi bigoye gukoresha. Ibi byateje icyitegererezo kidahuye, hamwe ningorane zo gupakirainama. Hano haribintu bishya biboneka ubu ariko, bikaba bikaba ari byiza cyane kandi bikajye muburyo bwo gukemura ibyo bibazo. Birakwiye kandi kubona ko nubwo imizigo y'amazi ishobora kuba idahwitse hamwe numuyoboro mwinshi muri rusange kurenza umuyoboro umwe kubera amakosa yabakoresha nkibisubizo byumunaniho ( Reba igika gikurikira).
Kugabanya ikosa ryabantu
Birashoboka ko amakosa ya muntu yagabanutse cyane kuko umubare wintambwe ya pietting ugabanuka. Biratandukanye numunaniro no kurambirwa byakuweho, bikavamo amakuru nibisubizo byizewe kandi byoroha.
Kalibrasi
Kugirango umenye neza ibikoresho byo gutunganya amazi, kalibration isanzwe irakenewe. Standard Iso8655 ivuga ko buri muyoboro ugomba kugeragezwa ukavuga. Imiyoboro myinshi umuyoboro ufite, igihe kirageze gifata kugirango gishobore kunywa igihe.
Nk'uko byatangajwe na Pipettecalib.net Bisanzwe 2.2 muri pipette ya 12 bisaba inzinguzingo 48 hamwe na gravimetric ya graviment (Umubumbe wa 2 x 2 gusubiramo x 12 cannels). Ukurikije umuvuduko wumukoresha, ibi birashobora gufata amasaha arenga 1.5 kuri pipette. Laboratoire mu Bwongereza isaba Calibration ya Uganda izakenera gukora uburemere bwa Gravimetrics 360 (Umubumbe wa 3 x 10 gusubiramo x 12 cannels). Gukora iyi mibare yibizamini bihinduka kandi birashobora kurenza igihe cyo kuzigama byagezweho ukoresheje umuyoboro mwinshi muri laboratoire.
Ariko, gutsinda ibyo bibazo serivisi za Pipette ziraboneka mumasosiyete menshi. Ingero zibi ni Gilson Labs, Thermofisher na Labut ya Pipette.
Gusana
Ntabwo arikintu benshi batekereza mugihe bagura pipette nshya, ariko provild yumuyoboro mwinshi ntabwo usasa. Ibi bivuze ko niba umuyoboro 1 wangiritse, provild yose irashobora gusimburwa. Ariko, abakora bamwe bagurisha abasimbuye imiyoboro ya buri muntu, bamenye neza ko basubiramo gusarura hamwe nuwabikoze mugihe ugura umuyoboro mwinshi.
Incamake - Nyimwe vs Umuyoboro mwinshi
Umuyoboro mwinshi muri pipette nigikoresho cyagaciro kuri buri laboratoire ufite ikindi kirenze kwinjiza inshuro nke cyane. Muburyo hafi ya buri kintu kinini cyamazi gisabwa kugirango theresti ari mubushobozi bwa buriinamakumuyoboro-wose wa pipette, kandi haribisubizo bike bifitanye isano nibi. Kwiyongera kwonyine gukomeye mugukoresha umuyoboro mwinshi warengewe cyane numuyoboro ugabanuka mubikorwa, bishoboza kugabanya umubare wintambwe zayimba. Ibi byose bivuze kunoza umukoresha ihumure, kandi ryagabanije ikosa ryumukoresha.
Igihe cyohereza: Ukuboza-16-2022