Pipette ni kimwe mu bikoresho bisanzwe bikoreshwa muri laboratoire y’ibinyabuzima, ivuriro, n’isesengura aho amazi agomba gupimwa neza no kwimurwa mugihe akora ibizamini, ibizamini cyangwa ibizamini byamaraso. Barahari nka:
Umuyoboro umwe cyangwa umuyoboro munini
Volume ingano yagenwe cyangwa ishobora guhinduka
Igitabo cyangwa ibikoresho bya elegitoroniki
Umuyoboro umwe-Umuyoboro umwe ni uwuhe?
Umuyoboro umwe uhuza abakoresha kwimura alikot imwe icyarimwe. Ibi bikunda gukoreshwa muri laboratoire bifite ibicuruzwa bike byintangarugero, bishobora kuba akenshi bigira uruhare mubushakashatsi niterambere.
Umuyoboro umwe umuyoboro ufite umutwe umwe wo kwifuza cyangwa gutanga urugero rwukuri rwamazi binyuze mumashanyaraziinama. Birashobora gukoreshwa mubikorwa byinshi muri laboratoire bifite gusa ibicuruzwa byinjira. Iyi ni laboratoire ikora ubushakashatsi bujyanye na chimie yisesengura, umuco w'akagari, genetiki cyangwa immunologiya.
Imiyoboro myinshi-Imiyoboro myinshi ni iki?
Imiyoboro myinshi-imiyoboro ikora muburyo bumwe nkumuyoboro umwe, ariko bakoresha byinshiinamayo gupima no gutanga amazi angana icyarimwe. Ibisanzwe bisanzwe ni imiyoboro 8 cyangwa 12 ariko imiyoboro ya 4, 6, 16 na 48 nayo irahari. Imiyoboro 96 yerekana imiyoboro irashobora kandi kugurwa.
Ukoresheje imiyoboro myinshi, biroroshye kuzuza byihuse 96-, 384-, cyangwa 1.536-nezaisahani ya microtiter, ishobora kuba irimo ingero zikoreshwa nka ADN amplification, ELISA (ikizamini cyo gusuzuma), ubushakashatsi bwa kinetic no gusuzuma molekile.
Umuyoboro umwe na Imiyoboro myinshi
Gukora neza
Umuyoboro umwe umuyoboro ni mwiza mugihe ukora imirimo yubushakashatsi. Ibi ni ukubera ko ahanini bikubiyemo gukoresha imiyoboro imwe, cyangwa guhuza rimwe kugirango ukore amaraso.
Ariko, ibi byihuse bihinduka igikoresho kidakora mugihe ibicuruzwa byiyongereye. Iyo hari ingero nyinshi / reagent zo kwimura, cyangwa ibinini binini birakoreshwa96 amasahani ya microtitre, hariho uburyo bwiza cyane bwo kohereza amazi hanyuma ukoresheje umuyoboro umwe. Ukoresheje imiyoboro myinshi ihuza aho, umubare wintambwe zo kugabanuka uragabanuka cyane.
Imbonerahamwe ikurikira irerekana umubare wintambwe zisabwa kugirango umuyoboro umwe, 8 na 12 ushyireho.
Umubare wintambwe zisabwa (6 reagents x96 Nibyiza Isahani ya Microtitre)
Umuyoboro umwe Pipette: 576
Umuyoboro wa 8-Umuyoboro: 72
Umuyoboro wa 12-Umuyoboro: 48
Ingano ya Pipetting
Itandukaniro rimwe ryingenzi hagati yumuyoboro umwe numuyoboro munini nubunini kuri buri riba rishobora kwimurwa icyarimwe. Nubwo biterwa nurugero rukoreshwa, mubisanzwe ntushobora kwimura amajwi menshi kumutwe kuri pipeti myinshi.
Ingano umuyoboro umwe wumuyoboro urashobora kwimura intera iri hagati ya 0.1ul na 10,000ul, aho urwego rwumuyoboro munini uri hagati ya 0.2 na 1200ul.
Icyitegererezo
Amateka, imiyoboro myinshi-imiyoboro itoroshye kandi kuyikoresha biragoye. Ibi byateje icyitegererezo cyo gupakira, hamwe ningorane zo gupakirainama. Hariho uburyo bushya buboneka ubu ariko, burushijeho gukoresha abakoresha kandi bugenda muburyo bwo gukemura ibyo bibazo. Twabibutsa kandi ko nubwo gupakira ibintu byamazi bishobora kuba bidahwitse gato hamwe numuyoboro uhuza imiyoboro myinshi, birashoboka cyane ko ari ukuri muri rusange kuruta umuyoboro umwe bitewe nuburyo budahwitse buturuka kumakosa yabakoresha biturutse kumunaniro ( reba igika gikurikira).
Kugabanya Ikosa ryabantu
Birashoboka ko ikosa ryabantu ryagabanutse cyane uko umubare wintambwe ugenda ugabanuka. Guhinduka kuva umunaniro no kurambirwa bivanwaho, bivamo amakuru nibisubizo byizewe kandi byororoka.
Calibration
Kugirango hamenyekane neza kandi neza neza ibikoresho bikoresha amazi, birasabwa kalibrasi isanzwe. Bisanzwe ISO8655 ivuga ko buri muyoboro ugomba kugeragezwa no gutanga raporo. Imiyoboro myinshi umuyoboro ufite, birebire bifata kugirango uhindure bishobora gutwara igihe.
Nkuko tubikesha pipettecalibration.net isanzwe ya kalibrasi ya 2.2 kumurongo wa 12 isaba inzinguzingo 48 hamwe nuburemere bwa gravimetric (umuzingo wa 2 x 2 gusubiramo x 12 imiyoboro). Ukurikije umuvuduko wumukoresha, ibi birashobora gufata amasaha arenga 1.5 kuri pipette. Laboratoire mu Bwongereza zisaba kalibrasi ya UKAS yakenera gukora uburemere bwa gravimetricike 360 (umuzingo wa 3 x 10 gusubiramo x 12 imiyoboro). Gukora uyu mubare wibizamini intoki biba bidashoboka kandi birashobora kurenza igihe cyo kuzigama cyagezweho ukoresheje imiyoboro myinshi ihuza muri laboratoire.
Ariko, kugirango ukemure ibyo bibazo serivise ya kalibrasi iraboneka mubigo byinshi. Ingero zibi ni Gilson Labs, ThermoFisher na Pipette Lab.
Gusana
Ntabwo ari ikintu abantu benshi batekereza mugihe baguze imiyoboro mishya, ariko ibice byinshi byumuyoboro munini ntibishobora gusanwa. Ibi bivuze ko niba umuyoboro 1 wangiritse, manifold yose irashobora gusimburwa. Nyamara, ababikora bamwe bagurisha abasimbuza imiyoboro yabantu, bityo rero menya neza niba ugomba gusana hamwe nuwabikoze mugihe uguze imiyoboro myinshi.
Incamake - Imiyoboro imwe-Imiyoboro myinshi
Imiyoboro myinshi-imiyoboro nigikoresho cyagaciro kuri buri laboratoire ifite ikintu kirenze ikintu gito cyinjiza cyintangarugero. Mubintu hafi ya byose urugero ntarengwa rwamazi asabwa kugirango yimurwe ari mubushobozi bwa buriinamakumuyoboro mwinshi, kandi haribibi bike bifitanye isano nibi. Kwiyongera kworoheje kugoye mugukoresha imiyoboro myinshi-imiyoboro irenze cyane kugabanuka kwa net kugabanuka kumurimo, bigashoborwa numubare wagabanutse cyane wintambwe. Ibi byose bivuze kunoza abakoresha neza, no kugabanya ikosa ryabakoresha.
Igihe cyo kohereza: Ukuboza-16-2022