Ni iki kigomba gusuzumwa mugihe P PCR ivanze PCR?

Kubijyanye no kongera ibintu byongeweho, birakenewe ko ibice byumuntu ku giti cye bihari muburyo bukwiye muri buri cyiteguro. Byongeye kandi, ni ngombwa ko kwanduza bibaye.

Cyane cyane mugihe ibitekerezo byinshi bigomba gushyirwaho, byashinzwe gutegura icyo bita Umwigisha uvanze aho kwiyuhagira buri reagent ukwayo muri buri cyombo. Inzego zinjijwe mbere ziboneka mubucuruzi, aho gusa ibigize icyitegererezo (primer) hamwe namazi yongeyeho. Ubundi, Umwigisha uvanze arashobora gutegurwa wenyine. Mubice byombi, imvange ikwirakwizwa kuri buri kintu cya PCR kitagira icyitegererezo hamwe nicyitegererezo cya ADN cyumwaka cyongeweho ukwayo.

Gukoresha Umwigisha ufite ibyiza byinshi: Icya mbere, umubare wintambwe imwe yagabanutse. Muri ubu buryo, ibyago byo gukoresha amakosa yabakoresha mugihe cyo gushushanya no kwanduza bigabanuka kandi, birumvikana ko igihe cyakijijwe. Ihame, ubutumwa bwuzuye kandi burenze, kubera ko ijwi rinini ritanga. Ibi biroroshye kubyumva mugihe ugenzura amakuru ya tekiniki ya pipettes: Gitoya Igisasu, hejuru cyane. Kuba imyiteguro yose ituruka ku bwato bumwe ifite ingaruka nziza kuri homogeneity (niba ivanze). Ibi kandi bikazamura indwara yubushakashatsi.

Mugihe witegura Umwigisha uvanga, byibuze 10% amajwi yinyongera agomba kongerwaho (urugero niba imyiteguro 10 ishingiye kuri 11), kugirango nubwato bwa nyuma bwuzuye neza. Muri ubu buryo, (buke) idahwitse, n'ingaruka zo gutakaza icyitegererezo iyo ugabanije ibisubizo birimo ibisubizo bishobora kuba byishyurwa. Ibikorwa bibi bikubiye mubisubizo bya enzyme nka Polymeyeses na shobuja bivanze, bigatera gushinga ibibyimba n'ibisigazwa ku buso bwimbere bwibisanzweinama za pipette.

Ukurikije porogaramu nuburyo bwamazi bigomba gutangwa, tekinike yukuri (1) igomba guhitamo nibikoresho bikwiye byatoranijwe. Kubisubizo birimo ibiryo, sisitemu yo kwimura itaziguye cyangwa ibyo bita "kugumana amafaranga make" Ingaruka zaACE Pipetteishingiye ku buso bwa hydrophobique. Ibinyobwa birimo ibikoresho ntibisiga firime yo gusiga imbere no hanze, kugirango gutakaza igisubizo.

Usibye gusangira neza ibice byose, ni ngombwa ko nta kwanduza imyiteguro bibaye. Ntabwo bihagije gukoresha ibishobora gukoreshwa cyane, kuko inzira yo gupakira mu kirere cya mu kirere gishobora kubyara aerotol ziguma muri pipette. ADN ishobora kuba indege ishobora kwimurwa kurugero rumwe kugeza kuruhande rwintambwe ikurikira bityo bigatuma kwanduza. Sisitemu yo kwimurwa mu buryo butaziguye haruguru irashobora kandi kugabanya izo ngaruka. Kubwumuyoboro wikirere birumvikana ko umvikana gukoresha inama zo kuyungurura kugirango urinde cone ya pipette mugumana amasuka, aerotol, na biomolecules.