ni iki mu gusuzuma vitro?

Muri vitro kwisuzumisha bivuga inzira yo gusuzuma indwara cyangwa imiterere ukurikije ibyitegererezo byibinyabuzima biva hanze yumubiri. Ubu buryo bushingiye cyane kuburyo butandukanye bwibinyabuzima, harimo PCR no gukuramo aside nucleic. Byongeye kandi, gufata amazi ni ikintu cyingenzi mu gusuzuma vitro.

PCR cyangwa polymerase urunigi reaction ni tekinike ikoreshwa mugukomeza ibice bya ADN. Ukoresheje primers yihariye, PCR itanga uburyo bwo guhitamo uburyo bwa ADN bukurikirana, bushobora gusesengurwa kubimenyetso byindwara cyangwa kwandura. PCR isanzwe ikoreshwa mugutahura virusi, bagiteri, fungal na parasitike, hamwe n'indwara zikomoka kuri kanseri.

Gukuramo aside Nucleic ni tekinike ikoreshwa mu gutandukanya no kweza ADN cyangwa RNA mu byitegererezo by’ibinyabuzima. Acide nucleic acide yakuweho noneho iraboneka kugirango irusheho gusesengura, harimo PCR. Gukuramo aside nucleique ningirakamaro mugupima neza no gutegura igenamigambi ryindwara zitandukanye.

Gutunganya amazi ni inzira ikubiyemo kwimura neza, gutanga no kuvanga ibintu bike byamazi muri laboratoire. Sisitemu yo gukoresha ibintu byikora byamenyekanye cyane mumyaka yashize kuko ituma byinjira cyane kandi byukuri mubisubizo nka PCR no gukuramo aside nucleic.

Muri vitro kwisuzumisha bishingiye cyane kuri ubwo buhanga bwa biologiya kuko butuma hamenyekana no gusesengura ibimenyetso bifitanye isano n'indwara hamwe na molekile. Kurugero, PCR irashobora gukoreshwa muguhuza gene yihariye ikurikirana ifitanye isano na kanseri yamabere, mugihe gukuramo aside nucleic bishobora gukoreshwa mugutandukanya ADN ikomoka kumibyimba bivuye mumaraso.

Usibye ubwo buhanga, ubundi buhanga nibikoresho bitandukanye bikoreshwa mugupima vitro. Kurugero, ibikoresho bya microfluidic bigenda bikoreshwa cyane-byinjira cyane hamwe na point-of-care. Ibi bikoresho byashizweho kugirango bikore neza kandi bikoreshe ibintu bike byamazi, bituma biba byiza kuri PCR nibindi bikorwa bya biologiya.

Mu buryo nk'ubwo, tekinoroji izakurikiraho (NGS) igira uruhare runini mu gusuzuma vitro. NGS ituma habaho guhuza ibice bya miriyoni z'ibice bya ADN, bigafasha kumenya vuba kandi neza kumenya ihinduka ry’imiterere ihindagurika. NGS ifite ubushobozi bwo guhindura indwara no kuvura indwara zikomoka kuri kanseri.

Muri make, muri vitro kwisuzumisha ni igice cyingenzi cyubuvuzi bugezweho kandi bushingira cyane kubuhanga bwibinyabuzima nka PCR, gukuramo aside nucleic, no gufata amazi. Izi tekinoroji, hamwe nikoranabuhanga nka microfluidic ibikoresho na NGS, birahindura uburyo bwo gusuzuma no kuvura indwara. Mugihe ikoranabuhanga rikomeje gutera imbere, muri vitro kwisuzumisha birashoboka ko bizagenda neza kandi neza, amaherezo bikazamura umusaruro wumurwayi nubuzima bwiza.

At Suzhou Ace Biomedical,twiyemeje kuguha ibikoresho bya laboratoire nziza cyane kubyo ukeneye byose bya siyansi. Urutonde rwinama za pipette, ibyapa bya PCR, imiyoboro ya PCR, hamwe na firime ya kashe byateguwe neza kandi bikozwe kugirango tumenye neza kandi neza mubushakashatsi bwawe bwose. Inama zacu za pipette zirahujwe nibirango byose byingenzi bya pipeti kandi biza mubunini butandukanye kugirango uhuze ibyo ukeneye byihariye. Ibyapa bya PCR hamwe nigituba bikozwe mubikoresho byujuje ubuziranenge kandi byashizweho kugirango bihangane ninzinguzingo nyinshi zumuriro mugihe gikomeza ubunyangamugayo. Filime yacu yo gufunga itanga kashe ikomeye kugirango irinde guhumeka no kwanduzwa nibintu byo hanze. Twunvise akamaro ko gutanga laboratoire yizewe kandi ikora neza, niyo mpamvu duharanira kuguha ibicuruzwa na serivisi nziza zishoboka. Itsinda ryinzobere ryacu rihora rihari kugirango rigufashe kubibazo cyangwa ibibazo ushobora kuba ufite.