Isahani ya PCR ni iki?

Isahani ya PCR ni ubwoko bwa primer, dNTP, Taq ADN polymerase, Mg, acide nucleic aside, buffer hamwe nabandi batwara bagize uruhare muri amplification reaction muri Polymerase Chain Reaction (PCR).

1. Gukoresha icyapa cya PCR

Ikoreshwa cyane mubijyanye na genetika, ibinyabuzima, ubudahangarwa, ubuvuzi, nibindi, ntabwo mubushakashatsi bwibanze gusa nko kwigunga kwa gene, gukoroniza no gusesengura aside nucleique, ariko no mugupima indwara cyangwa ahantu hose hari ADN na RNA. Nibikoreshwa rimwe muri laboratoire. Ibicuruzwa.

2.96 Nibyiza PCRIsahani

Ibikoresho byayo ahanini ni polypropilene (PP) muri iki gihe, kugirango irusheho kumenyera imiterere yubushyuhe bwo hejuru kandi buke murwego rwo kubyitwaramo PCR, kandi irashobora kugera ku bushyuhe bwinshi no guhagarika umuvuduko mwinshi. Kugirango ugere kubikorwa byinjira cyane bifatanije nimbunda yumurongo, imashini ya PCR, nibindi, plaque 96-iriba cyangwa 384-neza neza PCR ikoreshwa cyane. Imiterere ya plaque ihuye nubuziranenge mpuzamahanga bwa SBS, kandi kugirango ihuze nimashini za PCR zabakora inganda zitandukanye, irashobora kugabanywamo uburyo bune bwo gushushanya: nta skirt, igice kimwe cya kabiri, ijipo yazamuye hamwe nijipo yuzuye ukurikije igishushanyo mbonera.

3. Ibara nyamukuru ryicyapa cya PCR

Ibisanzwe birasobanutse kandi byera, muribyo byapa bya PCR byera bikwiranye nigihe gishya cya fluorescent yuzuye PCR.