Ni ubuhe buryo bwo kwirinda muguhindura pipette na burette?

Gupima neza amazi ni ngombwa kubushakashatsi bwa laboratoire, cyane mubice nkaubushakashatsi bwibinyabuzima, chimie, naimiti. Kugenzura ibikoresho nkaimiyoboronaburettesni ngombwa kugirango umenye neza neza akazi kawe. Waba ukora titre, kwimura amazi, cyangwa gukora isesengura ryimiti, ukurikiza ingamba zikwiye mugihe cya kalibibasi itanga ibisubizo bihamye, byizewe.

At Ace Biomedical, twumva akamaro ko gukoresha ibikoresho byiza-byo gupima neza. Muri iki kiganiro, tuzakuyobora binyuze mubikorwa byiza hamwe ningamba zingenzi zo guhagarika imiyoboro ya burettes na burettes, tumenye neza neza imikorere.

Gucukumbura ibyacuinamanibindi bikoresho bisobanutse, sura ibyacuurupapuro rwibicuruzwacyangwa wige byinshi kubyacuserivisi.

Calibration ni iki kandi ni ukubera iki ari ngombwa?

Calibration bivuga inzira yo kugenzura neza ibikoresho bya laboratoire ugereranije ibipimo byabo nibipimo bizwi. Kuri pipettes na burettes, ibi bivuze kwemeza ko ingano bapima cyangwa itanga ihuye namafaranga yagenewe bishoboka. Hatabayeho kalibrasi ikwiye, ibipimo bidahwitse birashobora kuganisha kubisubizo byubushakashatsi bidakwiye, niyo mpamvu kalibrasi isanzwe ari ngombwa.

Icyitonderwa cyo Guhindura Pipette

Umuyoboro ni igikoresho gisobanutse gikoreshwa mu kwimura ingano y’amazi. Kugirango umenye neza niba ari ukuri, kurikiza izi ngamba zingenzi mugihe cya kalibrasi:

1. Menya neza ko Pipette ifite isuku

Isuku ningirakamaro mugihe uhindura umuyoboro. Ibisigisigi byose cyangwa ibyanduye bisigaye imbere muri pipette uhereye kubikoresha mbere birashobora guhindura ibipimo. Sukura pipeti yawe neza ukoresheje ibikoresho byogusukura hanyuma ubyozeamazi yamenetsekwemeza ko nta miti yatinze.

2. Reba Ingaruka zubushyuhe ku Mubumbe

Ubushyuhe bugira ingaruka cyane kubunini bwamazi. Calibration igomba gukorwa ku bushyuhe bumwe aho pipeti izakoreshwa. Imiyoboro myinshi ihindurwamo ubushyuhe busanzwe bwa20 ° C kugeza kuri 25 ° C.. Niba ubushyuhe bwamazi butandukanye nurwego, birashobora kugira ingaruka kumajwi yatanzwe. Menya neza ko imiyoboro n'amazi byombi biri ku bushyuhe buhoraho kugirango wirinde kunyuranya.

3. Kuraho ibyuka byinshi

Umwuka mwinshi imbere muri pipette urashobora gutera amakosa akomeye yo gupima. Mbere ya kalibrasi, menya neza ko nta mwuka uhumeka uri muri barri ya pipeti. Kanda umuyoboro witonze cyangwa ubanze kugirango ukureho umwuka wafashwe. Ibi bizemeza ko pipette itanga ingano yukuri yamazi.

4. Koresha uburyo bukwiye bwo gufata neza

Uburyo ukoresha pipette mugihe cya kalibrasi birashobora kugira ingaruka zukuri kubipimo. Buri gihe ufate umuyoboro uhagaritse kugirango umenye neza amazi. Kugoreka umuyoboro urashobora gukurura amakosa mubunini, nibyingenzi rero kubyitwaramo ukurikije amabwiriza yabakozwe.

5. Reba niba ibyangiritse bigaragara

Mbere ya kalibrasi, genzura pipette ibyangiritse bigaragara, nk'ibice cyangwa ibisohoka. Ibyangiritse byose bishobora gutera ibipimo bidahwitse kandi bigomba guhita bikemurwa. Umuyoboro wangiritse ntukwiriye gupimwa neza, menya neza ko umeze neza mbere yo gukoreshwa.

6. Koresha Amazi azwi ya Calibration

Kugirango uhindure umuyoboro, koresha amazi afite ingano izwi, nkaamazi yamenetse. Gupima amazi yatanzwe na pipette hanyuma uyigereranye nagaciro kateganijwe. Niba hari ikinyuranyo, hindura pipette kugirango ihuze nubunini bukwiye. Kugenzura buri gihe bizafasha kugumya kumenya igihe.

7. Bika Pipette neza

Kubika neza nibyingenzi mugukomeza kalibrasi yawe. Mugihe udakoreshejwe, bika pipeti ahantu hizewe, humye, kure yimiti ikaze no kwangirika kwumubiri. Gukoresha ikibazo cyo kurinda cyangwa kubifata byemeza ko pipette ikomeza kumera neza kugirango ikoreshwe ejo hazaza.

Icyitonderwa cyo Guhindura Burette

Burette isanzwe ikoreshwa mugutanga ingano yuzuye yamazi mugihe cya titre cyangwa ubundi bushakashatsi. Guhindura neza burette bisaba kwitondera neza birambuye. Hano hepfo hari ingamba zingenzi ugomba gukurikiza mugihe uhindura burette:

1. Sukura Burette neza

Nka pipeti, burette igomba gusukurwa mbere ya kalibrasi. Ibisigisigi byose mubushakashatsi bwabanje birashobora kubangamira gupima. Sukura burette nezaamazi yamenetseno kwoza inshuro nyinshi kugirango ukureho umwanda wose.

2. Reba kuri Air Bubbles

Umwuka mwinshi imbere muri burette cyangwa nozzle birashobora kuganisha kumakosa akomeye yo gupima. Mbere yo guhinduranya, menya neza ko nta mwuka uhumeka. Uzuza burette amazi, hanyuma wemerere umwuka wose wafashwe uhunge ufunguye aho uhagarara, hanyuma utange amazi kugirango akureho ibibyimba.

3. Zeru Burette

Zeru burette nintambwe yingenzi muri kalibrasi. Iyo burette yuzuye, menya neza ko aho itangirira yashyizwe kuriikimenyetso cya zeru. Gutandukana kwose kuri zeru birashobora gutera ubusobanuro mubipimo byijwi mugihe cyo gukoresha. Buri gihe ugenzure burette iri kuri zeru mbere yo gutangira igeragezwa cyangwa gahunda yo guhitamo.

4. Koresha Amazi azwi ya Calibration

Kimwe na pipettes, kora burette ukoresheje ibipimo bizwi neza.Amazi yamenetseni isukari nziza kuriyi ntego kuko ifite ubucucike buzwi kandi byoroshye gupima. Nyuma yo kuzuza burette, tanga amazi muri silinderi yarangije hanyuma ugereranye amajwi nagaciro kateganijwe. Hindura kalibrasi ya burette niba habonetse itandukaniro.

5. Kugenzura aho uhagarara

Guhagarara bigenzura imigendekere yamazi ava muri burette. Menya neza ko ikora neza kandi idafite imyanda. Guhagarara nabi birashobora gutera urujya n'uruza, bikavamo gusoma nabi. Simbuza cyangwa usane aho uhagarara nibiba ngombwa kugirango ukore neza.

6. Shyira Burette Uhagaritse

Kugirango ugere kubipimo nyabyo, menya neza ko burette ihagaze neza mugihe cya kalibrasi. Kugoreka burette birashobora gutuma amazi atembera neza, biganisha kumakosa. Koresha igihagararo cya burette kugirango burette igumane neza kandi ukomeze guhuza verticale mugihe cya kalibrasi.

7. Soma neza Meniskus

Mugihe usoma urwego rwamazi muri burette, menya neza ko uriurwego rw'amasohamwe na menisk. Menisk ni ubuso bugoramye bwamazi, kandi kumazi menshi nkamazi, umurongo uzaba munsi. Soma ingingo yo hasi ya menisk kugirango umenye neza gusoma neza.

Guhinduranya buri gihe imiyoboro yombi na burettes ni ngombwa kugirango ugere ku bipimo nyabyo, nyabyo mu bushakashatsi bwa laboratoire. Ukurikije ingamba zavuzwe haruguru, uremeza ko ibikoresho byawe bitanga amakuru yizewe buri gihe. Niba urimo gukoraubushakashatsi bwibinyabuzima, gusesengura imiti, cyangwakwipimisha imiti, ibipimo byukuri byamazi nibyingenzi kugirango bigerweho.

At Ace Biomedical, twumva akamaro k'ibikoresho bya laboratoire yo mu rwego rwo hejuru. Iwacu inama nibindi bicuruzwa byateguwe kugirango byuzuze ibipimo bihanitse byukuri, byemeza ko ubushakashatsi bwawe butanga ibisubizo byiza. Kubindi bisobanuro, sura ibyacuUrupapuro, cyangwa niba ukeneye ubufasha, umva nezatwandikire.

Igihe cyo kohereza: Ukuboza-27-2024