Nibihe bikenewe bikoreshwa kubigeragezo byuzuye PCR?

Mubushakashatsi bwa genetike nubuvuzi, Polymeyese reaction (PCR) nubuhanga busanzwe bukoreshwa bwo kongera ADNI Ibyitegererezo bya ADLA kubigeragezo bitandukanye. Iyi nzira ishingiye cyane kuri PCR ibijyanye nibyingenzi kugirango uganire. Muri iki kiganiro, tuganira ku biguzi bya ngombwa PCR igerageza PCR: Ibyapa bya PCR, PCR ibiganiro bya SCR, byashyizwe ahagaragara, na papette.

PCR:

Plates plate nimwe mubishobora gukoreshwa cyane muri pcr iyo ari yo yose ya PCR. Bagenewe gusiganwa ku magare byihuse no gutanga imurika ry'ubushyuhe rimwe mu bireshwa kugirango borohereze. Isahani iraboneka muburyo butandukanye harimo 96-neza, 384 - neza, na 1536-neza.

Ibyapa bya PCR bikozwe muri plastike, bikaba bituma bizewe kandi byoroshye gukora. Byongeye kandi, ibyapa bimwe bya PCR bifatanye byumwihariko kugirango bibuza molekile ya ADN hanyuma wirinde kwanduza. Gukoresha amasahani ya PCR ni ngombwa kugabanya intambwe zingenzi zakozwe mbere muri microcentrifeges cyangwa imashini za PCR.

PCR TUBE:

PCR tubes ni imiyoboro mito, ubusanzwe ikozwe muri polypropylene, ikoreshwa muguhindura imvange ya PCR mugihe cyongerera. Baza mu mabara atandukanye, ariko ibintu birambuye birasobanutse kandi bisobanutse. Bisobanutse PCR bikoreshwa mugihe abakoresha bashaka kureba Applified Amplifient kuko baragikora.

Iyi miyoboro yashizweho kugirango ihangane n'ubushyuhe n'imitutu iboneka mu mashini za PCR, bigatuma biba byiza ku bushakashatsi bwa PCR. Usibye kongeramo, PCR tubes irashobora gukoreshwa mubindi bikorwa nkibikurikiranye bya ADN bikurikirana no kwezwa no gusesengura ibice.

Filime yo mu kadozi:

Filime ya kashe ni firime ya pulasitike ifatanye hejuru ya plate ya PCR cyangwa umuyoboro kugirango wirinde guhumeka no kwanduza imvange ya reaction mugihe cya PCR. Filime zikirangura ni ingenzi cyane mubushakashatsi bwa PCR, nkuko bigaragara ku buryo bugaragara cyangwa kwanduza ibidukikije mu isahani birashobora guhungabanya agaciro no gukora neza.

Bikozwe muri polyethylene cyangwa polypropylene, ukurikije porogaramu, izi firime za plastike zirahanganye cyane na autoclavable. Filime zimwe zaciwe kugirango hagabanyirizwe ibyapa byihariye bya PCR byihariye bya PCR, mugihe abandi baza kuzunguruka kandi birashobora gukoreshwa hamwe nibisahani bitandukanye bya PCR cyangwa imiyoboro itandukanye.

Inama za pipette:

Inama za Pipette ni ngombwa zikoreshwa mubushakashatsi bwa PCR, nkuko zikoreshwa mugushiramo amazi make, nka dingles cyangwa reagents. Mubisanzwe bikozwe muri polyethylene kandi birashobora gufata amajwi yubusa kuva 0.1 μl kugeza 10 ml. Inama za pipette zikoreshwa kandi zigenewe gukoreshwa rimwe gusa.

Hariho ubwoko bubiri bwamafaranga ya pipette - kubashutse kandi bitayungurujwe. Akayunguruzo k'ibikoresho birakwiriye gukumira aerosol cyangwa ibitonyanga bitangira, mugihe bitarimo inama zikoreshwa mugukoresha ubushakashatsi bwa PCR ukoresheje ibisubizo byihangana cyangwa ibisubizo bya Caustic.

Muri make, ibyapa bya PCR, PCR ibiganiro bya membrane, hamwe na pipette nimwe mubisabwa byibanze bikenewe kubigeragezo byuzuye pcr. Nukubyemeza ko ufite ibisabwa byose bikoreshwa, urashobora gukora neza PCR igerageza neza kandi hamwe nukuri ukeneye. Kubwibyo, burigihe reba neza ko ufite ibihagije kuri ibi bikoreshwa byoroshye kubigeragezo byose bya PCR.

At Suzhou Ace Biomedical, twiyeguriye kuguha ibikoresho byiza bya laboratoire nziza kubintu byose bya siyansi. Intera yacuinama za pipette, PCR, Pcr tubes, nafirimeByakozwe neza kandi byakozwe kugirango byemeze neza kandi neza mubushakashatsi bwawe bwose. Inama zacu za pipette zirahuye nibirango byose byingenzi bya pipettes hanyuma uze mubunini butandukanye kugirango uhuze ibyo ukeneye. Ibisahani bya PCR na tubes bikozwe mubikoresho byiza kandi byateguwe kugirango uhangane ninzinguzingo yubushyuhe mugihe ukomeje kuba inyangamugayo. Filime yo hejuru itanga kashe ihamye kugirango wirinde guhumeka no kwanduza ibintu byo hanze. Twumva akamaro k'ibikoresho byizewe kandi bikora neza, niyo mpamvu duharanira kuguha ibicuruzwa na serivisi nziza bishoboka. Itsinda ryacu ryimpuguke riraboneka kugirango rigufashe kubibazo cyangwa ibibazo ushobora kuba ufite.