Nibihe bikenerwa bikenewe kugirango igeragezwa ryuzuye rya PCR?

Mubushakashatsi bwerekeranye nubuvuzi nubuvuzi, polymerase urunigi (PCR) nubuhanga bukoreshwa muburyo bwo kongera urugero rwa ADN kubushakashatsi butandukanye. Iyi nzira iterwa cyane nibikoreshwa na PCR nibyingenzi kugirango bigerageze neza. Muri iki kiganiro, turaganira ku bintu byingenzi bikenerwa mu igeragezwa ryuzuye rya PCR: amasahani ya PCR, imiyoboro ya PCR, kashe ya kashe, hamwe ninama za pipette.

Isahani ya PCR:

Isahani ya PCR nimwe mubintu byingenzi bikoreshwa mubigeragezo byose bya PCR. Byashizweho kugirango ubushyuhe bwihuta bwamagare kandi butange ubushyuhe bumwe muri bore kugirango byoroshye gukemura. Isahani iraboneka muburyo butandukanye harimo 96-iriba, 384-neza, na 1536-neza.

Isahani ya PCR ikozwe muri plastiki, ituma yizewe kandi yoroshye kuyikora. Byongeye kandi, amasahani amwe ya PCR yashizwemo umwihariko kugirango abuze guhuza molekile ya ADN no kwirinda kwanduza. Gukoresha ibyapa bya PCR nibyingenzi mukugabanya intambwe yibikorwa byinshi byakozwe mbere muri microcentrifuges cyangwa imashini za PCR.

Umuyoboro wa PCR:

Imiyoboro ya PCR ni utubuto duto, ubusanzwe bukozwe muri polypropilene, ikoreshwa mu gufata PCR ivangavanga mugihe cya amplification. Ziza zifite amabara atandukanye, ariko ibisanzwe birasobanutse kandi byoroshye. Imiyoboro isobanutse ya PCR ikoreshwa kenshi mugihe abayikoresha bashaka kureba ADN yongerewe kuko iba iboneye.

Imiyoboro yabugenewe kugirango ihangane nubushyuhe bwinshi nigitutu kiboneka mumashini ya PCR, bigatuma biba byiza mubushakashatsi bwa PCR. Usibye kwongera imbaraga, imiyoboro ya PCR irashobora gukoreshwa mubindi bikorwa nko gukurikiranya ADN no kweza no gusesengura ibice.

Gufunga kashe:

Ikirangantego cya kashe ni firime ifata plastike ifatanye hejuru yisahani ya PCR cyangwa umuyoboro kugirango wirinde guhumeka no kwanduza imvange ya reaction mugihe cya PCR. Gufunga firime ni ingenzi cyane mubushakashatsi bwa PCR, kuko imvange yagaragaye cyangwa ibidukikije byose byanduye bishobora guhungabanya agaciro nubushakashatsi.

Ikozwe muri polyethylene cyangwa polypropilene, bitewe nibisabwa, izi firime za plastike zirwanya ubushyuhe bwinshi kandi zishobora kwizana. Filime zimwe zaciwe mbere kubisahani byihariye bya PCR hamwe na tebes, mugihe izindi ziza mumuzingo kandi zirashobora gukoreshwa hamwe nibisahani bitandukanye bya PCR.

Inama ya Pipette:

Impanuro za Pipette ningirakamaro zikoreshwa mubigeragezo bya PCR, kuko zikoreshwa mugutwara ibintu bike byamazi, nkurugero cyangwa reagent. Ubusanzwe bikozwe muri polyethylene kandi birashobora gufata urugero rwamazi kuva 0.1 µL kugeza 10 mL. Impanuro za Pipette zirashobora gukoreshwa kandi zigenewe gukoreshwa rimwe gusa.

Hariho ubwoko bubiri bwinama za pipette - zungurujwe kandi zitayungururwa. Akayunguruzo karakwiriye kugirango wirinde icyogajuru cyose cya aerosol cyangwa ibitonyanga bitabaho, mugihe inama zitayungurura zikoreshwa mubushakashatsi bwa PCR ukoresheje ibishishwa bidasanzwe cyangwa ibisubizo bya caustic.

Muncamake, plaque ya PCR, imiyoboro ya PCR, kashe ya membrane, hamwe ninama za pipette nibimwe mubintu byibanze bikenerwa bikenewe mubushakashatsi bwuzuye bwa PCR. Mugukora ibishoboka byose kugirango ukoreshe ibintu byose bikenewe, urashobora gukora neza igerageza rya PCR neza kandi hamwe nukuri ukeneye. Kubwibyo, burigihe menya neza ko ufite ibyo uhagije byoroshye kuboneka kubigeragezo byose bya PCR.

At Suzhou Ace Biomedical, twiyemeje kuguha ibikoresho byujuje ubuziranenge bya laboratoire kubyo ukeneye byose bya siyansi. Urwego rwacuinama, Ibyapa bya PCR, PCR tubes, nakashe ya firimebyateguwe neza kandi bikozwe neza kugirango ubone neza kandi neza mubushakashatsi bwawe bwose. Inama zacu za pipette zirahujwe nibirango byose byingenzi bya pipeti kandi biza mubunini butandukanye kugirango uhuze ibyo ukeneye byihariye. Ibyapa bya PCR hamwe nigituba bikozwe mubikoresho byujuje ubuziranenge kandi byashizweho kugirango bihangane ninzinguzingo nyinshi zumuriro mugihe gikomeza ubunyangamugayo. Filime yacu yo gufunga itanga kashe ikomeye kugirango irinde guhumeka no kwanduzwa nibintu byo hanze. Twunvise akamaro ko gutanga laboratoire yizewe kandi ikora neza, niyo mpamvu duharanira kuguha ibicuruzwa na serivisi nziza zishoboka. Itsinda ryinzobere ryacu rihora rihari kugirango rigufashe kubibazo cyangwa ibibazo ushobora kuba ufite.