CryOvialsbakunze gukoreshwa mububiko bwakorbic imirongo selile nibindi bikoresho bikomeye byabyaga ibinyabuzima, muri dewars yuzuye azote yamashanyarazi.

Hariho ibyiciro byinshi bigize uruhare mu kubungabunga selile za allicburine. Mugihe ihame shingiro ni uguhagarika buhoro, tekinike nyayo ikorwa biterwa n'ubwoko bwa karuri. Hariho ibitekerezo byinshi byumutekano nibikorwa byiza byo kuzirikana mugihe cyo kubika selile z'ubushyuhe bwo hasi.

Iyi nyandiko igamije gutanga incamake yukuntu Cryfovial ibitswe muri azote yuzuye.

Niki Crurtovials

Crytovials ni nto, yafashwe vial yagenewe kubika ingero zamazi mugihe cyubushyuhe buke cyane. Baremeza ko selile zibitswe muri Chiftrotectant ntukajye mu buryo butaziguye na azote y'amazi, kugabanya ibyago byo kuvunika kagari mugihe ugifite akamaro gakonje cyane ya azote yuzuye.

Ubusanzwe vials isanzwe iboneka murwego rwinshi nibishushanyo - birashobora kuba imbere cyangwa hanze yinkubi y'umuyaga cyangwa igitambaro. Imiterere mibi kandi idahwitse irahari.

UkoreshaCyrovialkubika selile muri azote

Urutonde rwa NHS na Laboratoire Yigenga, kimwe n'ibigo by'ubushakashatsi byihariye muri banki y'amaraso, ibinyabuzima bya epithelial, impumuroyi hamwe na sigari na Stem na Stem

Ingirabuzimafatizo zabitswe muri ubu buryo harimo b na t selile, cho selile, indabyo, monocyas, monocytes, moteri ya MESELMA, NK selile ya Stem

Incamake yububiko bwo kubika Cryovials muri azote yuzuye

KorOpreservavillavation ni inzira irinda selile nibindi binyabuzima bitera ibinyabuzima byo gukonjesha ubushyuhe buke cyane. Ingirabuzimafatizo zirashobora kubikwa muri azote yubusa imyaka myinshi nta gutakaza agaciro ka selile. Iyi ni urutonde rwuburyo bukoreshwa.

Imyiteguro ya selile

Uburyo nyabwo bwo gutegura ingero bizatandukana bitewe nubwoko bwakagari, ariko muri rusange, selile zikusanywa kandi zikarirwa kugirango utezimbere pellet. Iyi pellet noneho isukurwa muri sallenatant ivanze na chipdotectant cyangwa umukoreshateri.

Ubuyobozi bwa CryopReservation

Ubu buryo bukoreshwa bwo kubungabunga ingirabuzimafatizo mu bushyuhe buke-ibidukikije bizakorerwa no kubuza ishyirwaho rya intra na kristu ya kirisiti ya kirimbuzi bityo urupfu rwagajuru. Uruhare rwabo ni ugutanga ibidukikije bifite umutekano, kurinda ingirabuzimafatizo no mu ngingo mugihe cyo gukonjesha, kubika, no gutunganya inzira.

Uburyo bwa plasma nshya (FFP), yashutswe igisubizo cya Plasmalyte cyangwa Ibice byigenga, ibisubizo byubusa bivanze na Chimphidectants nka Dimethyl Sulphoxide nka Dimethyl Sulphixde nka Dimethyl Sulphixde (Dmsol.

Urupapuro rwicyitegererezo rwamakuru rukikijwe muri Cryovial Fritovial nkaSuzhou Ace Sosiyete Biomedical Cryogenic Kubika Vials.

Ni ngombwa kutirengagiza kuriko nkuko ibi bizongera ibyago byo gucika no kurekura ibishoboka (1).

Kugenzurwa

Muri rusange, igipimo kigenzurwa buhoro gikoreshwa kubwonko neza bwa kallsi.

Nyuma yingero zikikijwe muri Vials, zishyirwa ku rubura rutose cyangwa muri firigo ya 4 na 4 hamwe nuburyo bwo gukonjesha butangira muminota 5. Nkumuyobozi rusange, selile zikonje ku gipimo cya -1 kugeza -3 kumunota (2). Ibi bigerwaho ukoresheje ikonjesha cyangwa mugushyira vials mumasanduku yizewe yashyizwe muri A -70 ° C to -90 ° C Kugenzurwa Igipimo Cyimiryango.

Kwimura amazi ya azote

Viark yakonje noneho yimurirwa muri tank ya azote ya azote kubihe bitazwi byatanze ubushyuhe butarenze -135 ℃ birakomeza.

Ubu bushyuhe bwa ultra-buke burashobora kuboneka muguhuza mumazi cyangwa icyumba cya azose.

Amazi cyangwa icyuho?

Ububiko mu byifuzo byamazi azose bizwiho kubungabunga ubushyuhe bukonje hamwe nububasha bwuzuye, ariko akenshi ntibusabwa kubwimpamvu zikurikira:

• Gukenera byinshi (ubujyakuzimu) bwa azote yubusa ni akaga. Kutwika cyangwa guhumeka bitewe nibi ni ibyago nyabyo.
• Imanza zanditse zo kwanduza nabakozi banduye nka aspergillus, Hep B na virusi bakwirakwijwe hakoreshejwe uburyo bwa azote bwa azote (2,3)
• Ubushobozi bwa azote yubusa kugirango binjire muri valing mugihe cyibibazo. Iyo yakuwe mu bubiko no gususurutsa ubushyuhe bw'icyumba, azote yaguye vuba. Kubwibyo, vial irashobora gusenya iyo ikuwe mububiko bwa azote bwa azote, bitera akaga kuva kumyanda yombi iguruka kandi ihura nibirimo (1, 4).

Kubera izo mpamvu, Ultra-Ububiko buke bukunze kugaragara muri vapor icyiciro azose. Iyo ingero zigomba kubikwa mu cyiciro cyamazi, igituba cyihariye cyo kuvuza.

Ikibi ku cyiciro cyumwuka ni uko ubushyuhe buhagaritse gradient bushobora kubaho bikavamo ihindagurika ryubushyuhe hagati -135 ℃ na -190 ℃. Ibi bisaba gukurikirana neza kandi bishishikaye kurwego rwa azote ya azote nubushyuhe (5).

Abakora benshi barasaba ko Cryotovial ibereye kubika kugeza -135 ℃ cyangwa ngo ikoreshwe mu cyiciro cyuzuyemo gusa.

Gukoroho selile zawe

Uburyo bwo gutunganya buhangayikishije umuco ukonje, kandi uburyo bukwiye na tekinike birasabwa kwemeza ibidukikije byiza, gukira, no gukora neza kwa selile. Porotocole nyayo izaterwa nubwoko bwihariye. Ariko, gukomanga byihuse bifatwa nkibisanzwe kuri:

• Gabanya ingaruka zose ku gukira
• Fasha kugabanya igihe cyo kugaragara kuri Guhitamo Kugaragara Mubitangazamakuru byubukonje
• Mugabanye ibyangiritse kuri Ice Recrystallisation

Ubwiherero bw'amazi, Amasaro yo kwiyuhagira, cyangwa ibikoresho byihariye bikoreshwa muguhindura ingero.

Kenshi cyane umurongo wa selile wacyo waryoheye mugihe cyiminota 1-2, witonze kwiyuhagira amazi kugeza igihe hasigaye urumuri rwamazi gusa muri vial mbere yo gukaraba.

Ku tugari tumwe na tumwe nk'isoro ry'inyamabere, ubushyuhe buhoro ni ngombwa kugirango babeho.

Ubu selile ubu witeguye umuco w'akagari, kwigunga kwa selile, cyangwa ku bijyanye n'ingirabuzimafatizo za Haematoiiitic - ingendo za haematoiiic - ingendo zishinzwe kwemeza ubusugire bw'utugingo ngengabuzima mbere yo kuvura amatembere.

Nibikorwa bisanzwe byo gufata ibinyomoro bito byicyitegererezo cyamamaye kugirango ukore ikingirabuzimafatizo kugirango umenye ibitekerezo bya selile yo gutoragura umuco. Urashobora noneho gusuzuma ibisubizo byukuri byo kwigunga akanwa no kumenya akamaro ka seruke.

Imyitozo myiza yo kubika kuri Crityovials

Ibyiza byatsinze byimyidagaduro bibitswe muri Crityovial biterwa nibintu byinshi muri protocole harimo kubika no kubika inyandiko.

• Gutandukanya selile hagati yo kubika- Niba ingano yemewe, yagabanije selile hagati ya Valis hanyuma ubiteke ahantu hatandukanye kugirango igabanye ibyago byo gutakaza icyitegererezo kubera imigereka.
• Irinde kwanduza- hitamo gukoresha ingaragu chogenike sterile sterile cyangwa autoclave mbere yo gukoresha nyuma
• Koresha agaciro kanini kuri selile zawe- Vials iza muburyo butandukanye hagati ya 1 na 5mls. Irinde vial irenze kugirango ugabanye ibyago byo gucika intege.
• Hitamo imbere cyangwa hanze ya cloniogentic vial- Ikirangantego cyimbere hamwe na kaminuza zimwe zingana zumutekano - zirashobora kandi kwirinda kwanduza mugihe cyuzuye cyangwa mugihe ibitswe muri azote yuzuye.
• Irinde kumeneka- Koresha udusanduku twa Bi-twambajwe muri cap-cap cyangwa o-impeta kugirango wirinde gukubita no kwanduza.
• Koresha 2d barcode na label vial- Kugenzura amakuru, vial hamwe nibice binini byo kwandika bishobora gushoboza buri ngaruka kugirango ihagarikwa. 2D barcode irashobora gufasha mubuyobozi bwo kubika no kubika inyandiko. Ibara ryanditseho ingofero ni ingirakamaro kugirango byoroshye kumenyekana.
• Kubungabunga ububiko buhagije- Kugenzura niba selile zitazimiye, ibikoresho byo kubika bigomba guhora ukurikirana ubushyuhe no kurwego rwa aprogen. Impuruza zigomba gushyirwa kubakoresha amakosa.

Inganda z'umutekano

Amazi ya azote yabaye imyitozo mubushakashatsi bwa none ariko atwara ibyago byo gukomeretsa bikomeye niba byakoreshejwe nabi.

Ibikoresho bikwiye kugiti cyawe (PPE) bigomba kwambara kugirango ugabanye ibyago byo gukonjesha, gutwika hamwe nibindi bintu bibi mugihe ukoresha azote amazi. Kwambara

• Blogenic gants
• Ikote rya Laboratory
• Ingaruka zirwanya isura yuzuye yingabo nayo ikubiyemo ijosi
• Inkweto zifunze
• SplashipProof apron

Firigo ya firigo ya azote igomba gushyirwa mu turere duhujwe cyane kugira ngo bagabanye ibyago byo guhumeka - bahunze bihumeka kandi bimura ikirere cya ogisijeni. Ububiko bunini bwibumoso bugomba kugira sisitemu yo gutabaza ogisijeni.

Gukora muri babiri mugihe ukoresha Amazi nicyiza kandi ikoreshwa hanze yamasaha asanzwe yakazi agomba kubuzwa.

Crityovials kugirango ushyigikire akazi kawe

Suzhou Ace isosiyete ishinzwe ibinyabuzima itanga guhitamo ibicuruzwa byujuje ibikenewe kuri bortoprivisevation yawe muburyo butandukanye bwa selile. Portfolio ikubiyemo urutonde rwa Tubesas neza nkurutonde rwibintu bito.

Crytovials ni:

• Laborated chap 0.5ml 1.5ml 2.0ml chorotovial curgogenic vial storica dortonube hamwe na gasket

  .
  ● Igishushanyo mbonera cyangwa kwihagararaho, sterile cyangwa ntabwo ari sterile byombi birahari
  ● Inkweto za cap cap ikozwe mubyiciro byubuvuzi Polypropylene
  ● PP Conotonube Vials irashobora gukonjeshwa inshuro nyinshi kandi yakoroye
  Igishushanyo mbonera cyo hanze gishobora kugabanya kwanduza ibishoboka mugihe cyo kuvura.
  Cap chap chap clogenic itubasi rusange ya screw kugirango ikoreshwe
  ● Imiyoboro ihuye rotors zisanzwe
  .
  ● autoclavable kugeza 121 ° C kandi ihamye kuri -86 ° C.

  Igice oya	Ibikoresho	Ingano	CapIbara	PC /Umufuka	Imifuka / urubanza
  ACT05-BL-n	PP	0.5ml	Umukara, umuhondo, ubururu, umutuku, ibara ry'umuyugubwe, umweru	500	10
  ACT15-BL-N	PP	1.5ML	Umukara, umuhondo, ubururu, umutuku, ibara ry'umuyugubwe, umweru	500	10
  ACT15-BL-NW	PP	1.5ML	Umukara, umuhondo, ubururu, umutuku, ibara ry'umuyugubwe, umweru	500	10
  ACT20-BL-N	PP	2.0ml	Umukara, umuhondo, ubururu, umutuku, ibara ry'umuyugubwe, umweru	500	10