Amasaha angahe mucyumweru ubura amasahani menshi?
Urugamba nukuri. Nubwo imiyoboro cyangwa amasahani angahe wapakiye mubushakashatsi bwawe cyangwa akazi, ubwenge bwawe burashobora gutangira gukina amayeri mugihe cyo gupakira isahani 96 yimbitse.
Biroroshye cyane kongeramo amajwi kubwumurongo utari wo cyangwa umurongo mubi. Biroroshye gusa kubw'ibihe bibiri byisahani imwe.
Cyangwa ukorera icyitegererezo cyose mubiro byinshi, bigutwara amasaha menshi.
Cyangwa, birashoboka ko wakoze byose neza, ariko utangira kwikeba kabiri wenyine. Guhera.
Igihe cyawe ni ingirakamaro cyane. Imyitwarire yawe ifite agaciro kanini. Kandi, cyane cyane, amakuru yawe afite agaciro kanini.
Ntabwo tugomba kukubwira icyo guta igihe ibi aribyo, mugihe ubusanzwe ugomba kongera kuvuga reagents no kuvanga. Byongeye, ntabwo yumva bikomeye kurwego rwicyizere.
Hano hari inama nziza hamwe nabandi ushobora gutangira kwinjiza muri laborateur yawe.
Isahani ya 96 yimbitse?
Akenshi yirengagije muri laboratoire nubushakashatsi ahantu hose, amasahani maremare meza kubijyanye no kubika icyitegererezo cyigihe gito, kwitegura, no kuvanga. Bashobora kugira kare cyangwa hepfo.
Imikoreshereze yabo iratandukanye, ariko akenshi ikoreshwa mubumenyi bwa siyanse nubushakashatsi, harimo:
- Umuco wo muri Tissue Umuco wa Akagari no gusesengura Akagari
- Enzyme Assays
- Ubushakashatsi bwa Broteomics
- Ibigega bya Reagent
- Ububiko bw'icyitegererezo buringaniye (harimo na clogenic ububiko)
Inama zo hejuru & amayeri yo gutsinda amasaha ya 96 yimbitse
Twakusanyije urutonde rwa sisitemu yo hejuru no kubyegera muri bagenzi bawe:
- Reba imitekerereze yawe kandi ukomeze guhangayikishwa:Kimwe nibintu byose mubuzima, amakosa akunda kubaho mugihe unaniwe, uhangayitse, cyangwa urangaye (... cyangwa byose byavuzwe haruguru). Reka guhangayikishwa no kwihutisha umurimo wawe. Buhoro buhoro, kandi utekereze kuri buri ntambwe itonze. Kandi ukomeze kwibanda. Kuganira no gukora bituma imirimo runaka ijya vuba, ariko ntabwo ari hamwe niki gikorwa. Abashakashatsi bamwe bamanika "nta kuvuga" hejuru nkuko bari hagati yiki gikorwa. Umuziki uruhura (cyane cyane ibikoresho), ariko, birashishikarizwa niba ukeneye urusaku rwinyuma mugihe ukorera!
- Huza inama za pipette yo guhuza amariba:Agasanduku k'ubusambanyi bushya nibyiza kumasahani maremare. Huza iriba hamwe nagasanduku uko ugenda. Gira agasanduku k'Abitswe kuri standby mugihe wabuze, ntugomba rero guhungabanya sisitemu yawe niba ukeneye byinshi. Koresha inama za pipette kugirango ukurikirane neza.
- Andika:Kora urupapuro rwa Excel kubandi bavanze, na 96 amakarita yimbitse. Buri mucyo ufite izina rya primers nicyitegererezo. Shiraho inzamu yawe yose muburyo bwumvikana, hamwe namabara kuri buri primer yashyizweho (niba ukoresheje ibirenze). Zana uru rupapuro muri laboratoire, hanyuma urebe ikimenyetso urupapuro uko ugenda. Urashobora kandi kwandika amafaranga yinjira kumurongo wabyo hanyuma uyigumane kuruhande rwawe nkurugero rwawe rwicyitegererezo nkawe uremereye. Toranya sisitemu yo gukora binyuze muri bo (urugero: inyuguti cyangwa mubare, ukurikije uko banditse) kandi ntibigera bayobya sisitemu yawe. Mugihe uvanze, shyira ibintu byose murutonde rwawe, hanyuma uwimure mu mfuruka ya kure iyo urangiye.
- Tape ninshuti yawe magara:Kanda hejuru yisahani yose, kuruhande rwakarere urimo gupakira cyane. Kora ku isahani muri ubu buryo, wimura kaseti buri gihe igice kirangiye. Urashobora kuranga kaseti yawe (urugero - h, 1 - 12) kugirango igufashe kuguma kumurongo.
Kurugero, mugihe upakira gene mastermix mu nkingi yawe yimbitse, banza ufate kaseti kandi wihangane inkingi yitonze icyarimwe, kugirango ukore gahunda imwe, kugirango ugumane gahunda. Ifasha kuguma kwerekeza mu mari akomeye yo hagati. Gusa wibuke gufata isahani hasi mugihe ukuraho kaseti yawe, kugirango wirinde gucamo. - Komera hamwe nayo:Niba ubonye sisitemu yawe idakora, ntugahindure hagati. Hindura mbere cyangwa nyuma, ariko ntuzigere unyura hagati (biganisha ku rujijo cyane!).
- Imyitozo:Komeza bihuze nuburyo wahisemo. Gukora izi ntambwe zo kwibuka imitsi bizatwara igihe, ariko mugihe runaka ugomba gutangira kubona iterambere ryingenzi mubikorwa byawe (kandi ukabije cyane aho ukorera!)
Hitamo ibikoresho byiza:
Biturutse kubikoresho bigera ku bwiza, kuzenguruka cyangwa hepfo yumvikanye, hari amahitamo atandukanye mugihe utumiza isahani yimbitse.
Ibitekerezo bimwe birimo:
- Ibikoresho: Ni izihe ngero ukoresha? Birakenewe cyane kuba lobrind yambaye cyangwa siliconied?
- Ingano: Nubuhe bunini bugomba guhuza nisahani yawe ndende 96 PCR?
- Ubushyuhe: Ni ubuhe bushyuhe bukora amabenga wawe maremare akeneye kwihanganira?
- Ni izihe mbaraga z'ingabo zishobora gukora isahani iringaniye?
Dore ibyo abahanga benshi bakoresha porogaramu rusange:
Izi masahani yoroshye 96 yimbitse
Mbega ukuntu aya masahani yimbitse afasha Laborali na laboratoire:
- AnInzira yoroshyegukusanya no gutegura ingero (kubera ko ntabura kuri ibyo bintu bibaho muri laboratoire yawe buri munsi)
- Kubona Labspace nziza cyane, hamwe nubushobozi bukomeye bwo gufatanya kubika kuruta mbere hose
- Irinde kugorekaKunoza Kuvangay'amazi yawe mato
- IgishushanyoKugabanya Kugumana Urukuta, nuko utangiza bike murugero rwawe
- Kwishyura33%Kurenza ibyawe kubindi bicuruzwa biyobora
Ibiranga birimo:
- Hepfo
- Irashobora gukonjeshwa cyangwa gukonjesha (kugeza kuri -80 c)
- Guhagarara - Ntibazokwitwara nabi mu isahani
- Shyiramo nta byuma biremereye kugirango utezimbere neza
- Yateguwe hakurikijwe ubunini mpuzamahanga (SBS), bigatuma bikwiranye nibikorwa byikora
- Emera kugabanya amazi make ya sample yawe kurukuta
Guhitamo ikibanza cyiza birashobora kugufasha kwirinda:
- Yabuze amanota yamakuru
- Icyitegererezo rerun
- Umukozi watinze
- Kubura umushinga igihe ntarengwa
Kwidagadura
96 amasahani yimbitse aboneka muri laboratoire nubushakashatsi ku isi. Barashobora kuzigama umwanya, imbaraga, hamwe nu mwanya wo kubika, ariko sisitemu ikwiye ni ngombwa mugihe urangije akazi kawe.
Kuva mububiko bwiyongereye, kugirango uvange, amasahani yimbitse nibyiza kubikorwa bya chimie hamwe nibikorwa byisonga, birwanya imiti myinshi, ibicuruzwa bikoreshwa muri chinator.
Byiza gukusanya icyitegererezo, kwitegura icyitegererezo, n'igihe kirekire (cyangwa igihe gito) ububiko bwicyitegererezo, amasahani yimbitse hamwe nisahani yimbere nayo izagufasha gutanga amakuru menshi kuri Ubumenyi bwubuzima (ndetse no hanze).
Igihe cya nyuma: Gicurasi-10-2022