Nigute ushobora gufunga isahani ya PCR

Intangiriro

PCR, intangiriro ya laboratoire imyaka myinshi, zigenda ziyongera muburyo bugezweho nka laboratoire zipima ikoreshwa kandi rikarushaho gukoresha imyitozo mumurimo wabo. Kugera kuri izi ntego mugihe ubunyangamugayo bwubushakashatsi burashobora kugorana. Kimwe mu turere dusanzwe aho amakosa ashobora kunyerera ari hamwe no gushyirwaho ikimenyetsoPCR, hamwe nubuhanga bubi bwemerera guhumeka, guhindura pH hanyuma ugahagarika imirimo yubumenyi, no gutumira kwanduza. Kwiga uburyo bwo gufunga aPCRKurandura neza izi ngaruka kandi zicyemeza ibisubizo byo gutangiza.

Shakisha kashe iburyo kuri plate yawe ya PCR

Amasahani ya CAPS na Filime ya Filime Vs. lids
IngoferoNuburyo bwiza bwo gufunga isahani yawe hamwe na kashe yawe, mugihe ukomeje kuguha guhinduka byoroshye no kongera isahani nkuko ukeneye nta myanda. Ariko, ingofero zifite ibibi bibiri byingenzi.

Ubwa mbere, ugomba kugura ingofero yihariye ijyanye, itabagereranya. Uzagomba kumenya neza ko capite uhitamo ihuye nisahani, iterwa nuwabikoze, hanyuma uhitemo insanganyamatsiko cyangwa ikwirakwira kuri tranmocycle ukoresha.

Icya kabiri, gushyira mu bikorwa ku isahani birashobora gusubiramo cyane kandi birambiranye, bifite ibyago byo kwanduza niba ushyize umugongo utari kumwe ku iriba ritarishye.

Mugihe kashe ya firime ntabwo ihinduka mubijyanye no gukuraho no gusimbuza, biratandukanye cyane kuko bizahuza nisahani iyo ari yo yose ya PCR, ntabwo yubaha uwo uwabikoze. Bashobora guterwa gusa kubunini, bigatuma bakora neza.

Ubundi buryo ni isahani. Ibi bitanga uburinzi buke ko imipira na kashe, kandi bigakoreshwa cyane gusa mugihe gito kugirango birinde umwanda.

Optique na filique ya firime

Niba ukeneye ikimenyetso cyiza, gisobanutse cyangwa anFilime ya AluminiumGufunga isahani yawe byemejwe nuburyo bwawe bwo kugerageza.FilimeByanditsweho kugirango bikwemerera kwitegereza ingero, mugihe ukirinda no gukumira guhumeka. Bafite akamaro cyane mubushakashatsi bwa QPCR burimo gupima neza cyane ku isahani, muricyo gihe uzakenera firime yo mu kazura iyungurura ibicucu bike bishoboka. Ni ngombwa kumenya neza ko kashe cyangwa ingofero ukoresha ifite urwego ruhagije rwibisobanuro bya optique kugirango bikemuke ari ukuri.

Filime ya Foil irakwiriye ku byitegererezo icyo ari byo byose byoroshye cyangwa bigomba kubikwa muri 80 ° C. Kubera iyo mpamvu, ibyitegererezo byinshi bigenewe ububiko bwigihe kirekire bizakenera firime ya Foil. Filime za Foil nazo ziratobora, ni ingirakamaro kugirango usuzume amariba kugiti cye, cyangwa kugirango twohereze ingero ku nshinge. Ibi birashobora kubaho intoki cyangwa nkigice cyurubuga rwa robo.

Reba kandi ko ibintu bikaze birimo aside, ibishishwa cyangwa ibishoboka byose bizakenera kashe ishobora kubayihangana, muricyo gihe kashe ya Foil ishobora kuba ikwiye.

Amashanyarazi na firime yo gushyushya
Ikidodobagororotse cyane kandi byoroshye. Icyo ukeneye ni uwukoresha kugirango ukoreshe kashe ku isahani, hanyuma ukoreshe igikoresho cyoroshye cyo gukanda no gukora kashe ifatanye.

Ubushyuhe bukabije bugenda butera imbere, butanga kashe yanyuma yagabanije ibiciro bitandukanye ugereranije na kashe isanzwe. Ihitamo rirakwiye niba ushaka kubika ingero mugihe kirekire, nubwo ibi bizanwa nibisabwa byibikoresho byo hejuru.

Nigute ushobora gufunga isahani ya PCR

Uburyo bwo hejuru

Kwigira

1. Menya neza ko ukora ku mukoresha ukwiye kandi uhamye

2. Kuraho film uko yapakiye, hanyuma ukureho gutobora

3. Witondere ikidodo ku isahani, Mugihe urebe neza ko amariba yose atwikiriwe

4. Koresha igikoresho gisaba kugirango ushyire igitutu hejuru yisahani. Tangira kuva kumpera imwe hanyuma ukore inzira yawe kurundi, ukanda neza

5. Subiramo iki gihe kinini

6. Koresha usaba kwawe kurenga amariba hanze, kugirango umenye neza ko yashyizweho kashe neza.

Ubushyuhe

Ikidodo Shyushya Akazi ushonga firime kuri buri wese, hifashishijwe umupira wamasahani. Gukora umupira wamashure, reba amabwiriza yatanzwe nuwabikoze ibikoresho. Menya neza ko uwabikoze isoko ibikoresho byawe ava ahagaragara, kuko ari ngombwa cyane ko kashe ikwiye, ingirakamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ifite akamaro kandi ikora.

Isahani yo hejuru

a. Iyo ushyira ahagaragara kashe, jya mu cyerekezo cya horizontal na vertical kugirango urebe ko kashe ikwiye

b. Burigihe nibyiciro byiza byo gukora ikizamini cyikizamini cyose, kandi ibi ntaho bitandukaniye no gufunga isahani. Gerageza ukoresheje isahani yubusa mbere yo gukoresha imwe ifite ingero.

c. Iyo ugeragejwe, fata kashe urebe ko umurego watsimbaraye neza, nta cyuho. Hariho ishusho yerekana iyi nyandiko yambere. Niba utarangije isahani neza, iyo ukuyemo ikimenyetso hazabaho icyuho aho ibifatika bitangiriye neza ku isahani.

d. Yo kohereza no gutwara ingero, urashobora gusanga ari byiza gukoresha kashe ya plastike hejuru yikimenyetso cya foli kugirango ukingire (cyane cyane gutobora).

e. Burigihe menya neza ko nta bishishwa cyangwa inkeke mugihe ushyira kuri firime - ibi bizatera kumeneka no guhumeka