Nigute Pipette Umubumbe muto hamwe nintoki zintoki

Nkuko hibandwa cyane ku kugabanya reagent nigiciro, ingano ntoya irakenewe cyane, urugero, mugutegura PCR Mastermix cyangwa enzyme reaction. Ariko kuvoma ingano ntoya kuva 0.2 - 5 µL ishyiraho ibibazo bishya kubijyanye no kuvoma neza. Ingingo zikurikira ni ngombwa:

1. Ingano ya pipette nubunini: Buri gihe hitamo pipette nubunini buke bwizina rishoboka hamwe ninama ntoya kugirango umuyaga uhumeke neza bishoboka. Iyo kuvoma 1 µL urugero, hitamo 0.25 - 2.5 µL umuyoboro hamwe ninama ihuye aho kuba 1 - 10 µL.
2. Calibration no kubungabunga: Ni ngombwa ko imiyoboro yawe ihindurwa neza kandi ikabungabungwa. Guhindura bito n'ibice bimenetse kuri pipette biganisha ku kwiyongera gukabije muburyo butunguranye kandi butunguranye. Calibration ukurikije ISO 8655 igomba gukorwa rimwe mu mwaka.
3. Umuyoboro mwiza wo kwimura: Reba niba ufite pipeti nziza yo kwimura hamwe nubunini buke muri laboratoire yawe. Muri rusange, gukoresha ubu bwoko bwa pipette biganisha kumusubizo mwiza muburyo bwiza kandi busobanutse neza kuruta imiyoboro isanzwe yo mu kirere.
4. Gerageza gukoresha umubumbe munini: Urashobora gutekereza kugabanya urugero rwawe kuri pipette ingano nini hamwe nubunini bumwe mubisubizo byanyuma. Ibi birashobora kugabanya amakosa yo gutobora hamwe na sample ntoya cyane.

Usibye igikoresho cyiza, umushakashatsi agomba kuba afite tekinike nziza yo kuvoma. Witondere byumwihariko intambwe zikurikira:

1. Umugereka w'inama: Ntugahagarike umuyoboro hejuru yisonga kuko ibi bishobora kwangiza impera nziza ituma urumuri rwamazi rwerekanwa cyangwa rwangiza orifice. Gusa koresha igitutu cyumucyo mugihe wometse kumutwe hanyuma ukoreshe umuyoboro ufite isoko yuzuye isoko.
2. Gufata umuyoboro: Ntugafate pipette mu ntoki zawe mugihe utegereje centrifuge, cycler, nibindi. Imbere ya pipeti izashyuha kandi itume umusego wumwuka waguka bikavamo gutandukana nubunini bwagenwe mugihe cyo kuvoma.
3. Mbere yo guhanagura: Ubushuhe bwumwuka imbere muri tip na pipette bitegura inama yicyitegererezo kandi birinda guhumeka mugihe wifuza kohereza.
4. Icyifuzo gihagaritse: Ibi nibyingenzi cyane mugihe ukoresha amajwi mato kugirango wirinde ingaruka ya capillary ibaho mugihe pipette ifashwe kumurongo.
5. Ubujyakuzimu bwimbitse: Shira inama nkibishoboka kugirango wirinde amazi yinjira mumutwe kubera ingaruka ya capillary. Amategeko agenga igikumwe: Intoya nini nubunini, niko uburebure bwimbitse. Turasaba inama ntarengwa ya mm 2 mugihe dusohora ingano nto.
6. Gutanga kuri 45 ° inguni: Gusohoka neza mumazi byemewe mugihe umuyoboro ufashwe kuri 45 °.
7. Menyesha urukuta rw'ubwato cyangwa hejuru y'amazi: Umubumbe muto urashobora gutangwa neza mugihe inama ifashwe kurukuta rw'ubwato, cyangwa ikinjizwa mumazi. Ndetse igitonyanga cyanyuma kiva kumutwe kirashobora gutangwa neza.
8. Gusohora: Gusohora ni itegeko nyuma yo gutanga amajwi make kugirango utange ndetse nigitonyanga cyanyuma cyamazi kiboneka mumutwe. Igisasu nacyo kigomba gukorwa ku rukuta rw'ubwato. Witondere kutazana umwuka mubi murugero mugihe ukora ibisasu hejuru yamazi.