Nigute ushobora gupakira ingano ntoya hamwe nintoki zintoki

Nkibintu byinshi bishyirwaho kugabanya reagenke n'ibiciro, umuzingi muto urakenewe cyane, urugero, kugirango witegure PCR Mastermix cyangwa Enzyme reaction. Ariko umuyoboro muto uva kuri 0.2 - 5 μl zishyiraho ibibazo bishya byo gushushanya neza no gusobanuka. Ingingo zikurikira ni ngombwa:

1. Ingano hamwe ninama: Buri gihe uhitemo umuyoboro hamwe nubunini buke kandi bwimibare nto kugirango habeho umusego wo mu kirere ushoboka. Iyo umuyoboro wa 1 μl eg, hitamo 0.25 - 2.5 μl pipette kandi uhuye ninama aho kuba 1 - 10 μl pipette.
2. Calibration no kubungabunga: Ni ngombwa ko imigezi yawe ihinduka neza kandi ikomeza. Guhindura bito hamwe nibice byamenetse kuri pipette biganisha ku kwiyongera gukabije mubice bya sisitemu nagaciro. Calibration ukurikije ISO 8655 igomba gukorwa rimwe mumwaka.
3. Pipettes nziza: Reba niba ufite umuyoboro mwiza wa pipette hamwe nubunini buke muri laboratoire yawe. Muri rusange, ukoresheje ubu bwoko bwa pipette biganisha ku bisubizo byiza mubisubizo byukuri kandi neza kuruta hamwe na pipac yindege ya kera.
4. Gerageza gukoresha umubumbe munini: Urashobora gutekereza kurimbura icyitegererezo cyawe kuri pipette nini nini ifite ubwinshi mubikorwa byanyuma. Ibi birashobora kugabanya amakosa yo gushushanya hamwe nubunini buto cyane.

Usibye igikoresho cyiza, umushakashatsi agomba kugira tekiniki nziza cyane. Witondere bidasanzwe intambwe zikurikira:

1. Kugabanya inama: Ntukinjire umuyoboro ku cyiciro kuko ibi bishobora kwangiza inama nziza iherezo ritera urumuri rwamazi kugirango bayoborwe cyangwa kwangiza orifice. Gusa shyiramo igitutu cyoroheje mugihe uhuza inama kandi ukoreshe umuyoboro ufite isoko-yikorewe isoko cone.
2. Gufata pipette: Ntugafate umuyoboro mu ntoki mugihe utegereje ikinyagisirikare, cycler, nibindi imbere muri pipette bizashyuha no kuyobora umwobo wikirere kugirango uhagarike gutandukana uhereye kumurongo washyizweho mugihe wanditse.
3. Mbere yo guhamagara: Ubuhungiro bwumwuka imbere yimyandikire na Pipette bitegura inama yicyitegererezo kandi irinda guhumeka no kwirinda guhumeka iyo bifuza kwimura amajwi.
4. Ibyifuzo bihagaritse: Ibi nibyingenzi cyane mugihe ukoresha amajwi mato kugirango wirinde ingaruka mbi zibaho mugihe umuyoboro ufashwe kuruhande.
5. Kwibiza ubujyakuzimu: kwibiza inama gato ishoboka kugirango wirinde amazi yinjira mugihe cya capillary. Amategeko yintoki: ntoya inama nubunini, hepfo kwibiza ubujyakuzimu. Turasaba ntarengwa Mm 2 mugihe cyo gushushanya.
6. Gutanga kuri 45 ° Angle: Gukuramo ibintu byiza biremewe mugihe umuyoboro ukorwa kuri 45 °.
7. Twandikire Urukuta cyangwa hejuru yinyamanswa: Umubumbe muto urashobora gutanga neza mugihe inama ibaye ku rukuta rw'ibikoresho, cyangwa yibizwa amazi. Ndetse no kugabanuka kwa nyuma kuva ku shuri birashobora gutangwa neza.
8. Gukubita: Gukubita ni itegeko nyuma yo gutanga amajwi make kugirango atange igitonyanga cyanyuma cyamazi ahari. Gukubita bigomba no gukorerwa urukuta rw'ibikoresho. Witondere kutazana ibyumba byo mu kirere mu cyitegererezo mugihe ukora guhubuka hejuru yubusa.