Cryovial ni iki?
Ibikoresho byo kubika Cryogenicni ntoya, ifunze na silindrike yabugenewe yo kubika no kubika ingero kubushyuhe bukabije. Nubwo mubisanzwe ibyo bikoresho bikozwe mubirahure, ubu bikozwe cyane muri polypropilene kugirango byorohewe nimpamvu zibiciro. Cryovial yateguwe neza kugirango ihangane nubushyuhe buke nka -196 and, no kwakira ubwoko butandukanye bwubwoko. Ibi biratandukanye bivuye mugusuzuma ingirabuzimafatizo, mikorobe, selile yibanze kugeza kumurongo wimikorere. Hejuru y'ibyo, hashobora no kubaho ibinyabuzima bito byinshi bibitse imbereububiko bwa cryogenic, kimwe na acide nucleic na proteyine bigomba kubikwa kurwego rwubushyuhe bwa cryogenic.
Ibikoresho byo kubika Cryogenic biza muburyo butandukanye, kandi ugashaka ubwoko bwiza bwuzuza ibyo ukeneye byose bizemeza ko ukomeza ubudakemwa bwintangarugero utishyuye menshi. Soma unyuze mu ngingo yacu kugirango umenye byinshi kubyingenzi byingenzi byo kugura mugihe uhisemo cryovial ibereye gusaba laboratoire.
Ibyiza bya Cryogenic Vial yo gusuzuma
Inyuma vs Imbere
Abantu bakunze guhitamo ukurikije ibyo umuntu akunda, ariko mubyukuri hari itandukaniro ryingenzi ryimikorere yo gusuzuma hagati yubwoko bubiri bwurudodo.
Laboratwari nyinshi zihitamo inkono zometse imbere kugirango zigabanye umwanya wo kubika imiyoboro kugirango yemererwe neza mumasanduku ya firigo. Nubwo bimeze gurtyo, urashobora gutekereza ko amahitamo yinyuma yinyuma aribwo buryo bwiza kuri wewe. Bafatwa nk'utwara ibyago byo kwanduza hasi, bitewe nigishushanyo cyerekana ko bigoye ndetse nibindi byose bitari icyitegererezo kwinjira muri vial.
Inyuma zometse kumurongo zisanzwe zikundwa mubikorwa bya genomic, ariko amahitamo yombi afatwa nkibikwiye kuri biobanking hamwe nibindi byinjira byinjira cyane.
Ikintu cya nyuma ugomba gusuzuma kumutwe - niba laboratoire yawe ikoresha automatike, urashobora gukenera gusuzuma urudodo rushobora gukoreshwa hamwe na grippers.
Umubare wububiko
Ibikoresho bya Cryogenic biraboneka mubunini butandukanye kugirango bikemure byinshi bikenewe, ariko ahanini biri hagati yubushobozi bwa 1 mL na 5 mL.
Urufunguzo ni ukumenya neza ko cryovial yawe ituzuye kandi ko hari icyumba cyinyongera kiboneka, mugihe icyitegererezo cyabyimbye mugihe gikonje. Mu myitozo, ibi bivuze ko laboratoire ihitamo vial 1 mL mugihe ubitse ingero za 0.5 mL ya selile zahagaritswe muri cryoprotectant, hamwe na mili 2.0 mL kuri 1.0 mL yicyitegererezo. Indi nama yo kutuzuza amavatiri yawe nugukora gukoresha cryovial hamwe nibimenyetso byahawe impamyabumenyi, bizakwemeza ko wirinda kubyimba kwose bishobora gutera gucika cyangwa gutemba.
Kuramo Cap vs Flip Hejuru
Ubwoko bwo hejuru wahisemo buterwa ahanini nuburyo uzakoresha azote ya azote cyangwa idakoreshwa. Niba uri, noneho uzakenera screw capped cryovial. Ibi byemeza ko bidashobora gukingurwa kubwimpanuka kubera gufata nabi cyangwa ubushyuhe. Byongeye kandi, imipira ya screw itanga uburyo bworoshye bwo kugarura mumasanduku ya cryogenic no kubika neza.
Ariko, niba udakoresha azote ya azote kandi ukeneye hejuru byoroshye byoroshye gufungura, noneho flip top nuburyo bwiza. Ibi bizagukiza umwanya munini nkuko byoroshye gufungura, ibyo bigatuma ari ingirakamaro cyane mubikorwa byinjira cyane hamwe nibikoresha inzira.
Ikirango cy'umutekano
Inzira nziza yo kwemeza kashe itekanye ni ukumenya neza ko capo ya cryovial na icupa byombi byubatswe mubintu bimwe. Ibi bizemeza ko bagabanuka kandi bakaguka hamwe. Niba bikozwe hakoreshejwe ibikoresho bitandukanye, noneho bizagabanuka kandi byiyongere ku bipimo bitandukanye uko ubushyuhe buhinduka, biganisha ku cyuho gishobora gutemba hamwe n’ingaruka zanduye.
Ibigo bimwe bitanga ibyombo bibiri na flange kurwego rwo hejuru rwumutekano wicyitegererezo kuri cryovial yinyuma. O-Impeta ya cryovial ifatwa nkukuri kwizerwa imbere yimbere yimbere.
Ikirahure vs Plastike
Kubwumutekano no korohereza, laboratoire nyinshi ubu zikoresha plastike, mubisanzwe polypropilene, aho gukoresha amapule yikirahure. Ampules yikirahure ubu ifatwa nkihitamo ryataye igihe kuko mugihe cyo gufunga kashe ya pinhole itagaragara ishobora gutera, iyo ikonje nyuma yo kubika muri azote yuzuye ishobora kubatera guturika. Ntabwo kandi zikwiranye nubuhanga bugezweho bwo kuranga, urufunguzo rwo kwemeza icyitegererezo.
Kwihagararaho vs Hasi Hasi
Ibikoresho bya Cryogenic birahari byombi nko kwihagararaho hamwe ninyenyeri zimeze nkinyenyeri, cyangwa nkibizunguruka. Niba ukeneye gushyira vial yawe hejuru hejuru noneho urebe neza ko uhitamo kwihagararaho
Gukurikirana hamwe nicyitegererezo
Aka gace ko kubika cryogenic gakunze kwirengagizwa ariko icyitegererezo cyo gukurikirana no gukurikirana ni ikintu cyingenzi tugomba gusuzuma. Ingero za Cryogenic zirashobora kubikwa mumyaka myinshi, mugihe cyigihe abakozi bashobora guhinduka kandi badafite inyandiko zabitswe neza barashobora kutamenyekana.
Witondere guhitamo inzabya zituma icyitegererezo kimenyekana byoroshye bishoboka. Ibintu ugomba kureba harimo:
Ahantu hanini ho kwandika kugirango wandike amakuru ahagije kuburyo inyandiko zishobora kuboneka niba vial iherereye ahantu hadakwiye - mubisanzwe indangamuntu, itariki yahagaritswe, nintangiriro yumuntu ubishinzwe birahagije.
Barcode kugirango ifashe icyitegererezo cyo gucunga no gukurikirana sisitemu
Ingofero y'amabara
Icyitonderwa cy'ejo hazaza - chip-ultra-ubukonje irwanya ubukonje irimo gutegurwa, iyo ishyizwe muri cryovial kugiti cye, ishobora kubika amateka arambuye yubushyuhe kimwe namakuru arambuye yicyiciro, ibisubizo byikizamini nibindi byangombwa bifite ireme.
Usibye kuzirikana ibintu bitandukanye byerekana amavatiri aboneka, hari ibitekerezo bigomba no guhabwa uburyo bwa tekiniki bwo kubika cryovial muri azote yuzuye.
Ubushyuhe Ububiko
Hariho uburyo bwinshi bwo kubika kububiko bwa cryogenic bwintangarugero, buri kimwe gikora mubushuhe bwihariye. Amahitamo n'ubushyuhe bakoramo harimo:
Icyiciro cyamazi LN2: gumana ubushyuhe bwa -196 ℃
Icyuka cyumuyaga LN2: irashobora gukora mubushyuhe bwihariye buri hagati ya -135 ° C na -190 ° C bitewe nurugero.
Ubukonje bwa azote: -20 ° C kugeza -150 ° C.
Ubwoko bw'utugingo tubitswe hamwe nubushakashatsi bwatoranijwe nubushakashatsi buzagena uburyo butatu buboneka laboratoire yawe ikoresha.
Ariko, kubera ubushyuhe buke cyane bukoreshwa ntabwo imiyoboro yose cyangwa ibishushanyo bizaba byiza cyangwa umutekano. Ibikoresho birashobora gucika intege cyane mubushyuhe buke cyane, ukoresheje vial idakwiriye gukoreshwa mubushyuhe wahisemo bishobora gutuma ubwato buvunika cyangwa bukavunika mugihe cyo kubika cyangwa gushonga.
Witondere witonze ibyifuzo byabakora kubijyanye no gukoresha neza kuko viali zimwe na zimwe za kirogenike zikwiranye nubushyuhe buke nka -175 ° C, bamwe -150 ° C abandi 80 ° C.
Twabibutsa kandi ko abayikora benshi bavuga ko amavatiri yabo ya kirogenike adakwiriye kwibizwa mugice cyamazi. Niba ibyo bikoresho bibitswe mugice cyamazi mugihe usubiye mubushyuhe bwicyumba ibi bikoresho cyangwa kashe ya capa irashobora kumeneka bitewe nubwiyongere bwihuse bwumuvuduko uterwa no gutemba guto.
Niba ingirabuzimafatizo zigomba kubikwa mugice cyamazi ya azote yuzuye, tekereza kubika ingirabuzimafatizo zikoreshwa mubutaka bwa kirogenike zifunze ubushyuhe bwa kofoflex tubing cyangwa kubika ingirabuzimafatizo muri ampules zifunze zifunze.
Igihe cyo kohereza: Ugushyingo-25-2022