Ibiranga ubuziranenge bwa PCR: 0.1mL Yera 8-Strip ya PCR Ibisubizo byiza bya PCR

Mu rwego rwa biyolojiya y’ibinyabuzima, Polymerase Chain Reaction (PCR) ni tekinike yifatizo yahinduye uburyo bwo kwagura no gusesengura ibice byihariye bya ADN. Kugera ku bisubizo byiza bya PCR ntibisaba gusa ibikoresho na reagent gusa ahubwo biranakoreshwa neza cyane, cyane cyane imiyoboro ya PCR. Uyu munsi, nshimishijwe no kubamenyeshaACE'0.1mL Yera 8-Strip PCR Ibituba, byashizweho kugirango uzamure igeragezwa rya PCR kandi urebe neza ko bitagereranywa kandi byororoka. Reka twibire mubiranga inyungu ninyungu zituma iyi tubes ari umutungo wingenzi kubushakashatsi bwawe cyangwa laboratoire yo gusuzuma.

Kuberiki Hitamo ACE ya 0.1mL Yera 8-Strip PCR?

1.Ubwiza butagereranywa no guhuzagurika

Muri ACE, twishimiye kuba turi isoko rya mbere ryo gutanga ibikoresho byiza byo kwa muganga na laboratoire. 0.1mL Yera 8-Strip PCR Ibituba bikozwe hifashishijwe tekinoroji igezweho yo gutera inshinge hamwe nuburyo bukomeye bwo kugenzura ubuziranenge. Ibi byemeza ko buri muyoboro wujuje ubuziranenge bwo hejuru, uburinganire, hamwe nuburinganire buringaniye, byingenzi kubikorwa bya PCR bihoraho mubushakashatsi bwawe bwose.

2.Gukwirakwiza Porotokole ya PCR

Igishushanyo cyibikoresho byacu 8 byateguwe kugirango bihuze neza mumagare asanzwe yubushyuhe bwumuriro, bigabanya icyuho gishobora gutuma hashyuha kandi bikonje. Ubushobozi 0.1mL nibyiza kumurongo mugari wa porogaramu ya PCR, uhereye kumyanya isanzwe ya ADN kugeza kuri reaction nyinshi igoye, ukemeza ko ushobora kugera kubisubizo bikomeye kandi byororoka buri gihe.

3.Ibara ryera kugirango ryongere kugaragara

Ibara ryera ryibi bikoresho bya PCR ritanga uburyo bwiza bwo kugaragara ugereranije nigituba kibonerana, cyane cyane iyo ukorana nubunini buke cyangwa urugero rwa ADN yerekana urugero. Iyi mikorere itanga uburyo bworoshye bwo gukurikirana no kugenzura, kugabanya ibyago byo gukosora imiyoboro hamwe nicyitegererezo cyanduye.

4.Sterile na RNase / DNase-Ubuntu

Imiyoboro ya PCR iza kuba sterile kandi yemejwe na RNase / DNase-yubusa, itanga urwego rwinyongera rwo kwirinda aside nucleic. Ibi nibyingenzi byingenzi mugihe uhanganye nintego zoroshye cyangwa nkeya-nke, urebe ko ibisubizo byawe bitabangamiwe nuwanduye.

5.Ibidukikije-Byiza kandi birambye

ACE yiyemeje guteza imbere ibicuruzwa bishya kandi bitangiza ibidukikije. 0.1mL Yera 8-Strip PCR Ibituba bikozwe mubikoresho byujuje ubuziranenge, bisubirwamo, bigabanya ikirere cyibikorwa bya laboratoire. Hitamo ACE hanyuma utange umusanzu mugihe kizaza mubushakashatsi bwibinyabuzima.

6.Igiciro-Cyiza kandi Gupakira neza

Gupakirwa mumirongo umunani, utu tubari tubika umwanya muri firigo yawe no kuntebe ya laboratoire, bigatuma biba byiza cyane byinjira muri porogaramu za PCR. Imirongo irashobora gutandukanywa byoroshye mubituba kugiti cye, bigatanga guhinduka mubikorwa byawe mugihe wizeye neza.

Nigute ushobora gukoresha PCR yawe ukoresheje ACE ya PCR

Kugirango ukoreshe neza ibyiza bya 0.1mL ya ACE Yera 8-Strip PCR Ibituba, suzuma inama zikurikira:

1.Banza ukonje imiyoboro yawe: Shira imirongo muri cycle yumuriro mbere yo gutangira kwiruka kugirango ubushyuhe bwihuse kandi bumwe.

2.Koresha reagent nziza: Uzuza imiyoboro yawe hamwe na ACE ya reagent ya PCR kugirango wongere imikorere n'umusaruro.

3.Kugabanya umwuka: Menya neza ko ibipfundikizo bikora kashe ikomeye kugirango wirinde guhumeka, bishobora kugira ingaruka kubisubizo bya PCR.

4.Bika neza: Bika imiyoboro yawe kubushyuhe bwateganijwe kugirango ukomeze sterile na RNase / DNase-yubusa.

Umwanzuro

Gushora imari murwego rwohejuru rwa PCR ningirakamaro kugirango ugere kubisubizo byizewe kandi byororoka PCR. ACE ya 0.1mL Yera 8-Strip PCR Ibirahuri byateguwe kugirango byuzuze ibisabwa cyane mubushakashatsi bwibinyabuzima bigezweho no gusuzuma. Hamwe noguhuza kwukuri, guhuzagurika, hamwe ninshingano zibidukikije, utu tubari ni amahitamo meza yo kunoza ubushakashatsi bwa PCR. Suraurupapuro rwibicuruzwakugirango wige byinshi kandi utegeke ibyo utanga uyumunsi. Ongera ubushakashatsi bwa PCR hamwe na ACE yujuje ubuziranenge 0.1mL yera 8-imirongo ya PCR hanyuma ujyane ubushakashatsi bwawe kurwego rukurikira.