96-iriba ryiza cyane isahani (Isahani Yimbitse) ni ubwoko bw'isahani myinshi-isanzwe ikoreshwa muri laboratoire. Ifite umwobo wimbitse kandi ubusanzwe ikoreshwa mubushakashatsi busaba ingano nini yintangarugero cyangwa reagent. Ibikurikira nimwe mubyingenzi byingenzi bikoreshwa murwego hamwe nuburyo bukoreshwa bwa 96-iriba ryimbitse:
Urwego rusaba:
Kwipimisha cyane-Kwinjira: Mubigeragezo nko gusuzuma ibiyobyabwenge no gusuzuma isomero ryisomero, amasahani 96-yimbitse neza arashobora kwakira izindi ngero kandi bikazamura imikorere yubushakashatsi.
Umuco w'akagari: Bikwiranye nubushakashatsi bwumuco utugari busaba ubwinshi bwumuco uringaniye, cyane cyane umuco wingirabuzimafatizo.
Enzyme-ihuza immunosorbent assay (ELISA): Ikoreshwa mubushakashatsi bwa ELISA busaba ubunini bunini bwa sisitemu.
Ubushakashatsi bwibinyabuzima bya molekuline: nkibisubizo bya PCR, gukuramo ADN / RNA, gutegura icyitegererezo cya electrophoreis, nibindi.
Imvugo ya poroteyine no kwezwa: Byakoreshejwe mubigeragezo hamwe na proteine nini cyangwa bisaba ubunini bunini bwa buffer.
Ububiko bw'icyitegererezo bw'igihe kirekire: Bitewe n'uburebure bunini bw'imyobo, ihinduka ry'ijwi ry'icyitegererezo mugihe cyo gukonjesha rirashobora kugabanuka, rikwiriye kubikwa igihe kirekire.
Uburyo bukoreshwa:
Gutegura icyitegererezo: Ukurikije ibikenewe mu bushakashatsi, bapima neza urugero rukwiye rwa sample cyangwa reagent hanyuma ubyongere ku iriba ryisahani yimbitse.
Gufunga: Koresha firime cyangwa kashe ikwiye kugirango ushireho isahani kugirango wirinde guhumeka neza cyangwa kwanduzwa.
Kuvanga: Kunyeganyeza witonze cyangwa ukoreshe imiyoboro myinshi kugirango uvange icyitegererezo kugirango umenye neza ko icyitegererezo gihuye na reagent.
Inkubation: Shyira isahani yimbitse-isanduku yubushyuhe burigihe cyangwa ahandi hantu hakwiye kugirango hubakwe ukurikije ibisabwa mubushakashatsi.
Gusoma amakuru: Koresha ibikoresho nka microplate abasomyi na microscopes ya fluorescence kugirango usome ibisubizo byubushakashatsi.
Isuku no kuyanduza: Nyuma yubushakashatsi, koresha ibikoresho byogejwe kugirango usukure isahani yimbitse kandi uyanduze.
Ububiko: Isahani yimbitse igomba kubikwa neza nyuma yo koza no kuyanduza kugirango wirinde kwanduza.
Iyo ukoresheje 96-iriba ryimbitse-iriba, ingingo zikurikira nazo zigomba kwitonderwa:
Ibisobanuro byibikorwa: Kurikiza ibisobanuro bya aseptic kugirango wirinde kwanduza icyitegererezo.
Ukuri: Koresha umuyoboro munini cyangwa sisitemu yo gutunganya ibintu byikora kugirango utezimbere imikorere.
Ikimenyetso gisobanutse: Menya neza ko buri riba ryisahani ryerekanwe neza kugirango ryamenyekane byoroshye kandi byandikwe.
96-iriba-ryimbitseamasahani nigikoresho cyingenzi kubushakashatsi bwimbitse-muri laboratoire. Gukoresha neza birashobora kunoza cyane imikorere nukuri kwubushakashatsi.
Igihe cyo kohereza: Kanama-13-2024