Mu rwego rwubushakashatsi bwa siyanse, cyane cyane mubice nka biohimiya, ibinyabuzima by’utugingo ngengabuzima, na farumasi, guhitamo ibikoresho bya laboratoire birashobora kugira ingaruka zikomeye ku mikorere n’ubushakashatsi. Kimwe mu byemezo nkibyo ni uguhitamo hagati y-amariba 96 na 384. Ubwoko bwibisahani byombi bifite inyungu zabyo nibishobora kugaruka. Urufunguzo rwo kunoza imikorere ya laboratoire ni ukumva itandukaniro no guhitamo icyiza gikenewe cyihariye cyibigeragezo.

1. Umubare ninjiza

Kimwe mu bintu by'ibanze bitandukanya 96-iriba na 384-amasahani ni umubare w’iriba, bigira ingaruka ku buryo butaziguye ingano ya reagent ishobora gukoreshwa no kwinjiza ubushakashatsi. Isahani 96-iriba, ifite amariba manini, mubisanzwe ifite amajwi menshi, bigatuma ikwiranye nubushakashatsi busaba reagent nyinshi cyangwa ingero, hamwe nubushakashatsi aho guhumeka bishobora gutera impungenge. Ibinyuranye, amasahani 384-meza, hamwe nubucucike bwayo bwinshi bwamazi, yemerera umubare munini wibisubizo icyarimwe, bityo ukongera ibicuruzwa cyane. Ibi nibyiza cyane muburyo bwo kwinjiza ibicuruzwa byinshi (HTS), aho ubushobozi bwo gutunganya umubare munini wintangarugero byihuse.

2. Gukora neza

Igiciro nikindi kintu gikomeye tugomba gusuzuma. Mugihe amasahani 384-amariba akenshi yemerera gukora byinshi kuri buri sahani, bishobora kugabanya igiciro kuri buri cyegeranyo, birashobora kandi gusaba ibikoresho byuzuye kandi bihenze cyane byo gukoresha amazi. Byongeye kandi, ingano ntoya ya reagent ikoreshwa mumasahani 384-iriba irashobora gutuma uzigama amafaranga menshi kuri reagent mugihe. Ariko, laboratoire igomba guhuza ibyo kuzigama hamwe nishoramari ryambere mubikoresho byateye imbere.

3. Ibyiyumvo nubuziranenge bwamakuru

Ibyiyumvo byubushakashatsi bwakozwe muri 96-iriba na plaque 384-birashobora kandi gutandukana. Mubisanzwe, ingano nini muri plaque 96-nziza irashobora gufasha kugabanya guhinduka no kuzamura umusaruro wibisubizo. Ibi bituma bakora ibigeragezo aho precision yibanze. Ku rundi ruhande, amasahani 384-yuzuye, hamwe nubunini buto, arashobora kongera ibyiyumvo mubisubizo bimwe na bimwe, nka fluorescence cyangwa luminescence ishingiye kubisubizo, bitewe nibimenyetso byinshi.

4. Gukoresha Umwanya

Umwanya wa laboratoire akenshi uba uri hejuru, kandi guhitamo isahani birashobora guhindura uburyo uyu mwanya ukoreshwa neza. Isahani 384-isahani ituma ubushakashatsi bwinshi bukorerwa mumwanya umwe ugereranije nibisahani 96-byiza, bikagaragaza neza intebe ya laboratoire hamwe na incubator. Ibi birashobora kugirira akamaro cyane muri laboratoire ifite umwanya muto cyangwa aho ibikorwa-byinjira cyane ari ngombwa.

5. Guhuza ibikoresho

Guhuza nibikoresho bya laboratoire bihari nibindi bitekerezo byingenzi. Laboratwari nyinshi zimaze kugira ibikoresho bigendanye n'amasahani 96-meza, kuva robobo yohereza imashini kugeza kubasoma amasahani. Kwimukira ku byapa 384-byiza birashobora gusaba ibikoresho bishya cyangwa guhindura sisitemu zisanzwe, zishobora kubahenze kandi zitwara igihe. Kubwibyo, laboratoire igomba gusuzuma neza niba inyungu zo guhinduranya amasahani 384 iruta izo mbogamizi.

Umwanzuro

Ubwanyuma, icyemezo kiri hagati yo gukoresha amariba 96-cyangwa 384-amariba ashingiye kubisabwa byihariye bya laboratoire n'imiterere y'ubushakashatsi burimo gukorwa. Kubushakashatsi bukeneye ubunini bunini kandi aho sensitivite no kubyara ari ngombwa, amasahani 96-meza arashobora guhitamo neza. Ibinyuranye, kubisaba byinshi-byinjira kandi bigakoreshwa neza muburyo bwo gukoresha reagent, amasahani 384-meza arashobora kuzamura imikorere ya laboratoire. Laboratoire zigomba gusuzuma neza ibyo bintu, urebye imiterere yihariye, kugirango uhitemo amakuru neza kandi meza.