Hitamo uburyo bwa plaque ya PCR

Isahani ya PCR mubisanzwe ikoresha 96-neza na 384-nziza, ikurikirwa na 24-iriba na 48-neza. Imiterere yimashini ya PCR yakoreshejwe hamwe nibisabwa biri gukorwa bizagaragaza niba isahani ya PCR ikwiranye nubushakashatsi bwawe.
Skirt
“Skirt” ya plaque ya PCR ni isahani ikikije isahani. Ipati irashobora gutanga ituze ryiza kubikorwa byo kuvoma mugihe cyo kubaka sisitemu yo kubyitwaramo, kandi igatanga imbaraga zumukanishi mugihe cyo gutunganya imashini. Isahani ya PCR irashobora kugabanywamo amajipo, igice cyijipo hamwe nijipo yuzuye.
Ubuso bwibibaho
Ubuso bwibibaho bivuga hejuru yacyo.
Igishushanyo mbonera cyuzuye kibereye imashini nyinshi za PCR kandi biroroshye gufunga no gukora.
Igishushanyo mbonera cya plaque gifite uburyo bwiza bwo guhuza nibikoresho bimwe na bimwe bya PCR, bifasha kuringaniza umuvuduko wigitutu cy'ubushyuhe bidakenewe adapteri, bigatuma ihererekanyabubasha ryiza hamwe nubushakashatsi bwizewe.
Ibara
Ibyapa bya PCRmubisanzwe biraboneka muburyo butandukanye bwamabara atandukanye kugirango byoroherezwe gutandukanya amashusho no kumenya ingero, cyane cyane mubushakashatsi bwimbitse. Nubwo ibara rya plastike ridafite ingaruka ku kwongera ADN, mugihe dushyizeho igihe nyacyo cya PCR, turasaba gukoresha ibikoresho bya pulasitike byera cyangwa ibikoresho bya pulasitiki bikonje kugira ngo tugere kuri fluorescence yoroheje kandi yuzuye ugereranije n’ibikoreshwa mu mucyo. Ibiryo byera byongera ibyiyumvo no guhuza amakuru ya qPCR mukurinda fluorescence kwanga kuva mumiyoboro. Iyo kugabanuka kugabanutse, ibimenyetso byinshi bigaragarira kuri detector, bityo bikongerera ibimenyetso-urusaku. Byongeye kandi, urukuta rw'umuyoboro wera rurinda ibimenyetso bya fluorescent kwanduzwa muri module y'ibikoresho bya PCR, birinda kwinjizwa cyangwa kwerekana ibimenyetso bya fluorescente, bityo bikagabanya itandukaniro mubushakashatsi bwakorewe.
Ibirango bitandukanye byibikoresho, kubera igishushanyo gitandukanye cyumwanya wa detector ya fluorescence, nyamuneka reba kuri manuf