Inzobere muri laboratoire zirashobora kumara amasaha buri munsi zifata micropipette, no kunoza imikorere ya pipine no kwemeza ibisubizo byizewe akenshi ni ikibazo. Guhitamo micropipette ibereye kubisabwa byose ni urufunguzo rwo gutsinda imirimo ya laboratoire; ntabwo yemeza gusa imikorere yubushakashatsi ubwo aribwo bwose, ariko kandi byongera imikorere.Kutumva ibikenewe byogukora imiyoboro ituma abayikoresha bahitamo imiyoboro nyayo kandi isubirwamo, ariko hariho nibindi bintu byinshi bigomba kwitabwaho kugirango tunonosore ibisubizo kandi byemeze ko bizagenda neza ubushakashatsi.
Muri rusange, amazi ari mubyiciro bitatu byingenzi: amazi, ibishishwa, kandi bihindagurika. Amazi menshi ashingiye kumazi, bigatuma imiyoboro yo kwimura ikirere ihitamo bwa mbere kuri benshi.Mu gihe amazi menshi akora neza hamwe nubwoko bwa pipeti, imiyoboro ya volumetric igomba guhitamo mugihe gukorana n'amazi meza cyane cyangwa ahindagurika.Itandukaniro riri hagati yubwoko bwa pipette ryerekanwe ku gishushanyo cya 1.Ni ngombwa kandi gukoresha tekinike nziza yo kuvoma - utitaye ku bwoko bwamazi - kubisubizo byiza.
Ibice bibiri byingenzi bigira ingaruka kubisubizo byogusohora ni ukuri kandi neza (Igicapo 2) .Kugera kumurongo ntarengwa wo gutobora neza, neza, no kwizerwa, ingingo nyinshi zigomba kuzirikanwa. Nkuko amategeko abigenga, uyikoresha agomba guhitamo buri gihe pette ntoya ibyo birashobora gukemura ingano yimurwa.Ibi nibyingenzi uko ubunyangamugayo bugabanuka uko ingano yashyizweho yegera ingano ntoya ya pipette.Urugero, niba utanze 50 µl hamwe na 5,000 µl pipette, ibisubizo birashobora kuba bibi.Ibisubizo byiza birashobora kuba byabonetse hamwe na 300 µl imiyoboro, mugihe 50 µl imiyoboro itanga ibisubizo byiza.Ikindi kandi, ingano yashyizwe kumiyoboro gakondo yintoki irashobora guhinduka mugihe cyo kuvoma bitewe no guhinduranya kubwimpanuka ya plunger.Niyo mpamvu bamwe mubakora inganda zapanze uburyo bwo gufunga amajwi kugirango bakumire. impinduka zitabishaka mugihe cyo gutobora kugirango turusheho kwemeza neza.Kalibibasi nikindi kintu cyingenzi gifasha kwemeza ibisubizo byizewe mugaragaza neza na neza neza ya pipeti.Iyi nzira igomba kuba yoroshye kubakoresha; kurugero, imiyoboro imwe ya elegitoronike irashobora gushyiraho kwibutsa kalibrasi, cyangwa kubika amateka ya kalibrasi.Ntabwo ari imiyoboro gusa yo gusuzuma. Niba inama ya pipette irekuye, ikameneka, cyangwa igwa, irashobora gutera ibibazo bitandukanye. Iki kibazo gikunze kugaragara muri laboratoire. bikunze guterwa no gukoresha inama rusange-yintego ya pipette, ikenera akenshi "gukanda." Iyi nzira irambuye inkombe yumutwe wa pipette kandi irashobora gutuma inama imeneka cyangwa igasimburwa, cyangwa bikanatuma inama igwa kuri pipeti burundu .Guhitamo micropipette yo mu rwego rwohejuru yateguwe hamwe ninama zihariye zituma ihuza ryizewe kurushaho, ritanga urwego rwo hejuru rwo kwizerwa hamwe nibisubizo byiza. Byongeye kandi, ikintu cyoroshye nkibikoresho byerekana amabara hamwe ninama bishobora kandi gufasha abakoresha kwemeza ko inama zukuri zatoranijwe kuri imiyoboro yabo.
Mubidukikije byinjira cyane, ni ngombwa gukora neza bishoboka mugihe ukomeje kwizerwa no guhuzagurika mubikorwa byo kuvoma.Hariho inzira nyinshi zo kunoza imikorere ya pipine, harimo no gukoresha imiyoboro myinshi na / cyangwa imiyoboro ya elegitoronike.Ibikoresho bitandukanye. akenshi itanga uburyo butandukanye bwo guhinduranya - nko guhinduranya imiyoboro ihindagurika, gutanga impinduka zitandukanye, gahunda yo guhinduranya gahunda, nibindi byinshi - kugirango byorohereze inzira.Urugero, inzira nko gutanga inshuro nyinshi nibyiza mugutanga alikoti nyinshi zingana zingana utarinze kuzuza inama. Gukoresha umuyoboro umwe kugirango wohereze ingero hagati yuburyo butandukanye bwa laboratoire irashobora guhita irambirana cyane kandi ikunda kwibeshya. Imiyoboro ya Multichannel yemerera kwimura ingero nyinshi icyarimwe mu kanya nk'ako guhumbya. Ntabwo aribyo byongera imikorere gusa, bifasha no gukumira imiyoboro. amakosa hamwe no gukomeretsa inshuro nyinshi (RSI) .Pipeti zimwe na zimwe zifite ubushobozi bwo guhinduranya intera mugihe cyo kuvoma, kwemerera ihererekanyabubasha ryikigereranyo cyinshi hagati yubunini bwa laboratoire nuburyo butandukanye, bikiza amasaha yigihe (Ishusho 3).
Inzobere muri laboratoire zimara amasaha kumunsi zipompa.Ibi birashobora gutera ikibazo kandi, mubihe bikomeye, ndetse no gukomeretsa amaboko cyangwa ukuboko. Inama nziza yo kwirinda izo ngaruka zishobora kugabanywa ni ukugabanya igihe ufata pipeti mugihe gito gishoboka. . Usibye ibi, abakoresha bagomba guhitamo micropipette yoroheje kandi iringaniye neza hamwe na misa muri centre kugirango ituze neza. Umuyoboro ugomba guhuza neza mumaboko yabakoresha ibumoso-iburyo, bafite igishushanyo mbonera cyiza, kandi bahindura amajwi neza kandi byihuse bishoboka kugirango wirinde kugenda bitari ngombwa.Ikindi kandi, inama ni ngombwa, kuko imitwaro yo gupakira no gusohora akenshi bisaba imbaraga zirenze imiyoboro kandi hari ingaruka zishobora gukomeretsa, cyane cyane mugushira hejuru cyane. Inama zipapuro zigomba gufata mu mwanya hamwe nimbaraga nke, tanga ihuza ryizewe, kandi byoroshye gusohora.
Mugihe uhisemo micropipette ibereye kubyo usaba, ni ngombwa kureba buri kintu cyose cyakazi cyawe. Urebye pipette, ibiyiranga, ubwoko nubunini bwamazi arimo gutwarwa, hamwe ninama zikoreshwa, abahanga barashobora kwemeza neza, neza kandi byizewe ibisubizo mugihe ukomeza umusaruro no kugabanya ibyago byo gukomeretsa.
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Igihe cyo kohereza: Jun-10-2022