Inzobere muri laboratoire zirashobora kumara amasaha buri munsi zifata micropipette, no kunoza imikorere ya pipine no kwemeza ibisubizo byizewe akenshi ni ikibazo. Guhitamo micropipette ibereye kubisabwa byose ni urufunguzo rwo gutsinda imirimo ya laboratoire; ntabwo yemeza gusa imikorere yubushakashatsi ubwo aribwo bwose, ariko kandi byongera imikorere.Kutumva ibikenewe byogukora imiyoboro ituma abayikoresha bahitamo imiyoboro nyayo kandi isubirwamo, ariko hariho nibindi bintu byinshi bigomba kwitabwaho kugirango tunonosore ibisubizo kandi byemeze ko bizagenda neza ubushakashatsi.
Muri rusange, amazi ari mubyiciro bitatu byingenzi: amazi, ibishishwa, kandi bihindagurika. Amazi menshi ashingiye kumazi, bigatuma imiyoboro yo kwimura ikirere ihitamo bwa mbere kuri benshi.Mu gihe amazi menshi akora neza hamwe nubwoko bwa pipeti, imiyoboro ya volumetric igomba guhitamo mugihe gukorana n'amazi meza cyane cyangwa ahindagurika.Itandukaniro riri hagati yubwoko bwa pipette ryerekanwe ku gishushanyo cya 1.Ni ngombwa kandi gukoresha tekinike ikwiye - utitaye ku bwoko bwamazi - kubisubizo byiza.
Ibice bibiri byingenzi bigira ingaruka kubisubizo byogusohora ni ukuri kandi neza (Igicapo 2) .Kugera kumurongo ntarengwa wo gutobora neza, neza, no kwizerwa, ingingo nyinshi zigomba kuzirikanwa. Nkuko amategeko abigenga, uyikoresha agomba guhitamo buri gihe pette ntoya ibyo birashobora gukemura ingano yimurwa.Ibi nibyingenzi uko ubunyangamugayo bugabanuka uko ingano yashyizweho yegera ingano ntoya ya pipette.Urugero, niba utanze 50 µl hamwe na 5,000 µl pipette, ibisubizo birashobora kuba bikennye.Ibisubizo byiza birashobora kuboneka hamwe na 300 µl pipettes, mugihe 50 µl imiyoboro itanga ibisubizo byiza.Ikindi kandi, ingano yashyizwe kumiyoboro gakondo y'intoki irashobora guhinduka mugihe cyo kuvoma bitewe no kuzunguruka kubwimpanuka ya plunger.Niyo mpamvu bamwe mubakora amapeti yateye imbere gufunga amajwi yoguhindura ibishushanyo kugirango wirinde impinduka zitabishaka mugihe cyo gutobora kugirango turusheho kwemeza neza. Calibration ni ikindi kintu cyingenzi gifasha kwemeza ibisubizo byizewe byerekana ukuri nukuri neza kwa pipette. Iyi nzira igomba kuba yoroshye kubakoresha; kurugero, imiyoboro imwe ya elegitoronike irashobora gushyiraho kwibutsa kalibrasi, cyangwa kubika amateka ya kalibrasi.Ntabwo ari imiyoboro gusa yo gusuzuma. Niba inama ya pipette irekuye, ikameneka, cyangwa igwa, irashobora gutera ibibazo bitandukanye. Iki kibazo gikunze kugaragara muri laboratoire. akenshi biterwa no gukoresha inama rusange-yintego ya pipette, ikenera akenshi "gukanda." Iyi nzira irambuye inkombe yumutwe wa pipette kandi irashobora gutuma inama imeneka cyangwa igasimburwa, cyangwa igatera inama kugwa. umuyoboro wuzuye. Guhitamo micropipette yo mu rwego rwohejuru yateguwe hamwe ninama zihariye zituma habaho guhuza umutekano kurushaho, bitanga urwego rwo hejuru rwo kwizerwa hamwe nibisubizo byiza. Byongeye kandi, ikintu cyoroshye nkibikoresho byerekana amabara hamwe ninama bishobora gufasha abakoresha kumenya inama zukuri. Byahiswemo Kuri pipeti zabo.
Mubidukikije byinjira cyane, ni ngombwa gukora neza bishoboka mugihe ukomeje kwizerwa no guhuzagurika mubikorwa byo kuvoma.Hariho inzira nyinshi zo kunoza imikorere ya pipine, harimo no gukoresha imiyoboro myinshi na / cyangwa imiyoboro ya elegitoronike.Ibikoresho bitandukanye. akenshi itanga uburyo butandukanye bwo guhinduranya - nko guhinduranya imiyoboro ihindagurika, gutanga ibintu bihinduka, gahunda ihindagurika, hamwe nibindi - kugirango byoroshe inzira.Urugero, inzira nko gutanga inshuro nyinshi nibyiza kuri gutanga alikoti nyinshi zingana zingana utarinze kuzuza inama. Gukoresha imiyoboro imwe yumuyoboro umwe kugirango wohereze ingero hagati yuburyo butandukanye bwa laboratoire irashobora guhita irambirana cyane kandi ikunda kwibeshya. Ntabwo ibyo byongera imikorere gusa, bifasha no gukumira amakosa yo gutembera no gukomeretsa inshuro nyinshi (RSI) .Imiyoboro imwe nimwe ifite ubushobozi bwo gutandukanya intera ihanamye mugihe cyo kuvoma, ikemerera kubangikanya ihererekanyabubasha ryinshi hagati yubunini bwa labware nuburyo butandukanye, bizigama amasaha yigihe (Ishusho 3).
Inzobere muri laboratoire zimara amasaha kumunsi zipompa.Ibi birashobora gutera ikibazo kandi, mubihe bikomeye, ndetse no gukomeretsa amaboko cyangwa ukuboko. Inama nziza yo kwirinda izo ngaruka zishobora kugabanywa ni ukugabanya igihe ufata pipeti mugihe gito gishoboka. . Usibye ibi, abakoresha bagomba guhitamo micropipette yoroheje kandi iringaniye neza hamwe na misa muri centre kugirango ituze neza. Umuyoboro ugomba guhuza neza mumaboko yabakoresha ibumoso n’iburyo, bafite igishushanyo mbonera cyiza, kandi hindura amajwi neza kandi byihuse bishoboka kugirango wirinde kugenda bitari ngombwa.Ikindi kandi, inama ni ngombwa, kuko imitwaro yo gupakira no gusohora akenshi bisaba imbaraga zirenze imiyoboro kandi hari ingaruka zishobora gukomeretsa, cyane cyane mugushiraho ibicuruzwa byinshi. fata ahantu hamwe nimbaraga nkeya, utange ihuza ryizewe, kandi byoroshye gusohora.
Mugihe uhisemo micropipette ibereye kubyo usaba, ni ngombwa kureba buri kintu cyose cyakazi cyawe. Urebye pipette, ibiyiranga, ubwoko nubunini bwamazi arimo gutwarwa, hamwe ninama zikoreshwa, abahanga barashobora kwemeza neza, neza kandi byizewe ibisubizo mugihe ukomeza umusaruro no kugabanya ibyago byo gukomeretsa.
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Igihe cyo kohereza: Jun-10-2022