Nigute ushobora guhitamo ibyapa bya PCR hamwe na tebes kugirango ubisabe?

Inzira ya polymerase (PCR) nubuhanga bukoreshwa cyane mubinyabuzima bwa molekuline kugirango hongerwe ibice bya ADN. PCR ikubiyemo intambwe nyinshi, zirimo gutandukana, guhuza, no kwagura. Intsinzi yubu buhanga iterwa ahanini nubwiza bwa plaque ya PCR hamwe nigituba gikoreshwa. Hariho ibintu byinshi ugomba gusuzuma muguhitamo ibyapa bya PCR hamwe nigituba gikwiye cyo gusaba.Dore ibintu bimwe byingenzi ugomba gusuzuma:

1. UbushoboziIbyapa bya PCRna tebes biza mubunini n'ubushobozi butandukanye. Guhitamo ingano nubushobozi biterwa ahanini nubunini bwa ADN bugomba kongerwa muburyo bumwe. Kurugero, niba ukeneye kongera umubare muto wa ADN, urashobora guhitamo umuyoboro muto. Niba umubare munini wa ADN ukeneye kongerwa, isahani ifite ubushobozi bunini irashobora gutoranywa.

2. Ibikoresho bya PCR hamwe nigituba birashobora gukorwa mubikoresho bitandukanye nka polypropilene, polyakarubone cyangwa acrylic. Polypropilene nibikoresho bikoreshwa cyane kubera imiti nubushyuhe bwayo. Ntabwo kandi bihenze ugereranije nibindi bikoresho. Polyakarubone na acrylics zihenze cyane, ariko zifite optique nziza kandi nziza kuri PCR mugihe nyacyo.

3. Ubushyuhe bwumuriro PCR burimo uruziga rwinshi rwumuriro, bisaba gushyuha byihuse no gukonjesha kuvanga reaction. Kubwibyo, isahani ya PCR hamwe nigituba bigomba kugira ubushyuhe bwiza kugirango habeho gushyuha no gukonjesha kuvanga reaction. Isahani ifite inkuta zoroheje hamwe nubuso buringaniye nibyiza byo kohereza ubushyuhe bwinshi.

4. Guhuza ibyapa bya PCR hamwe nigituba bigomba guhuzwa na cycle yumuriro ukoresha. Isahani hamwe nigituba bigomba kuba bishobora guhangana nubushyuhe bwo hejuru busabwa kugirango hongerwe ibice bya ADN. Buri gihe ujye ubaza uruganda rukora amashyanyarazi kubisahani hamwe na tebes.

5. Ikidodo Ikidodo gifatika ningirakamaro kugirango wirinde kwanduza imiti ivanze. Isahani ya PCR hamwe nigituba birashobora gufungwa hakoreshejwe uburyo butandukanye nka kashe yubushyuhe, firime zifata cyangwa umupfundikizo. Gushyushya ubushyuhe nuburyo bwizewe kandi butanga inzitizi ikomeye yo kurwanya umwanda.

6. Sterilisation ya plaque ya PCR nigituba bigomba kuba bitarimo umwanda uwo ariwo wose ushobora kubangamira reaction. Kubwibyo, bigomba guhindurwa mbere yo kubikoresha. Ni ngombwa guhitamo amasahani hamwe nigituba byoroshye guhagarika kandi birwanya uburyo bwa chimique nubushyuhe.

Muri make, guhitamo icyapa cya PCR hamwe nigituba ningirakamaro kugirango ADN igerweho neza. Guhitamo biterwa ahanini nubwoko bwa porogaramu, ingano ya ADN yongerewe, hamwe no guhuza amagare yumuriro.

Suzhou Ace Biomedical Technology Co., Ltd.. itanga urutonde rwibikoresho byiza bya PCR hamwe nigituba mubunini butandukanye, ubushobozi nibikoresho kugirango buri mushakashatsi akeneye.