Nigute wahitamo amasahani ya PCR ikwiye kubisabwa?

Urunigi rwa Polymeyese (PCR) nubuhanga bukoreshwa cyane muri biologiya ya molecular kugirango yongere ibice bya ADN. PCR ikubiyemo intambwe nyinshi, harimo gusenyuka, gukomera, no kwaguka. Intsinzi yubuhanga buterwa ahanini nubwiza bwa plate na tubes ikoreshwa. Hariho ibintu byinshi ugomba gusuzuma mugihe uhitamo amasahani ya PCR ikwiye.Hari hari ibintu byingenzi ugomba gusuzuma:

1. UbushoboziPCRna tubes baza mubunini nubushobozi butandukanye. Guhitamo ingano nubushobozi biterwa ahanini na ADN igomba kongerwa muburyo bumwe. Kurugero, niba ukeneye kongera umubare muto wa ADN, urashobora guhitamo umuyoboro muto. Niba umubare munini wa ADN ugomba gushimangirwa, isahani ifite ubushobozi bunini irashobora gutoranywa.

2. Ibyapa bya PCR na tube birashobora gukorwa mubikoresho bitandukanye nka polypropylene, polycarbonate cyangwa acrylic. Polypropylene nibikoresho bikunze gukoreshwa bitewe nuburozi nubushyuhe. Birahenze kandi ugereranije nibindi bikoresho. Polycarbonates na Acrylics birahenze cyane, ariko bafite ibisobanuro byiza kandi nibyiza kuba PCR nyayo.

3. PCRMAL Itwara Ubushyuhe PCR ikubiyemo inzinguzingo yubushyuhe bwinshi, bisaba gushyushya vuba no gukonjesha imvange ivanze. Kubwibyo, amasahani ya PCR na tubes agomba kuba afite imyitwarire myiza yubushyuhe kugirango akureho imyenda yo gushyuha no gukonjesha imvange. Amasahani hamwe nurukuta ruto nubuso bunini ni bwiza bwo kwimura ubushyuhe.

4. Guhuza amasahani ya PCR na tubes bigomba guhuzwa na cycler yubushyuhe ukoresha. Ibisahani na tubes bigomba kuba bashoboye kwihanganira ubushyuhe bwo hejuru busabwa kugirango bikorogure ibice bya ADN. Buri gihe ujye ubaza urubuga rwumuyaga wa Cycler kubisahani basanze amanota.

5. Shyira akadomo gakomeye ni ingenzi kugirango wirinde kwanduza imvange ivanze. Ibyapa bya PCR na tubes birashobora gushyirwaho ikimenyetso ukoresheje uburyo butandukanye nka kashe yubushyuhe, firime zifatika cyangwa umupfundikizo. Ubushyuhe bwo gushinga ubushyuhe nuburyo bwizewe kandi butanga inzitizi ikomeye kwirinda kwanduza.

6. Ndumirwa amasahani ya PCR na tubes bagomba kuba badafite impumuro nziza zishobora kubangamira reaction. Kubwibyo, bagomba gucika intege mbere yo gukoreshwa. Ni ngombwa guhitamo amasahani hamwe na tubes byoroshye kumuhogeje no kurwanya uburyo bwo gushyushya imiti nubushyuhe.

Muri make, guhitamo isahani iboneye PCR na tubes nibyingenzi kubikorozwa bya ADN. Guhitamo biterwa ahanini nubwoko bwo gusaba, umubare wa ADN Yongerewe, kandi uhuza na cycle.

Suzhou Ace Biomedical Congol Co, Ltd. Tanga uburyo butandukanye bwa plate-yo hejuru ya pcr hamwe nibisobanuro muburyo butandukanye, ubushobozi nibikoresho kugirango duhuze ibyifuzo bya buri mushakashatsi.