Urunigi rwa Polymeyese (PCR) nimwe mu buryo buzwi cyane bukoreshwa muri laboratoire yubumenyi yubumenyi.
Ibyapa bya PCR bikozwe muri plastique yambere yo gutunganya no gusesengura ingero cyangwa ibisubizo byakusanyijwe.
Bafite inkuta zinanutse kandi zishingiye gusa kugirango batange ubushyuhe busobanutse.
Mugutegura ibihe nyabyo Porogaramu, igice cyiminota ya ADN cyangwa RNA bitangwa kandi bibitswe mumasahani ya PCR.
Ibyapa bya PCR biroroshye cyane mubushyuhe no kugabanya urujya n'uruza.
Ariko, nkingirakamaro kandi yizewe amasahani ya PCR ni, ikosa nibidashikarizwa byoroshye mugihe cyo gutunganya ingero.
Kubwibyo, niba ushishikajwe no kubona ubuziranenge kandi bworoshyePCR.Nibyiza kuvugana nu mpeshyi yizewe PCR. Hamwe nibi urizera ko ugira amasezerano meza.
Hano hari ingamba zo gukurikiza kugirango wirinde kwanduza imisoro cyangwa ingero no gukumira bidahwitse kuva ku bisubizo.
Fungura ibidukikije
Ibitekerezo bitari byo cyangwa ibibi bibaho bitewe no kuba hari umwanda, bigutera gushidikanya kubisubizo.
Umwanda kandi wanduye bibaho muburyo butandukanye nka ADN idafitanye isano cyangwa inyongera yimiti amaherezo igabanya imikorere myiza hamwe nuburyo bwo kubyitwaramo.
Hariho inzira nyinshi zo kugabanya cyane igipimo cyo kwanduza isahani ya PCR.
Gukoresha inama zo kuyunguruzo nindi buryo bwingirakamaro kugirango wirinde umwanda wo kunyerera mu byitegererezo byawe ukoresheje pipettes.
Tanga ibikoresho bisukuye rwose, bigizwe na pipettes na rack, gusa kugirango bakoreshe PCR. Ibi bizatanga ihererekanyabundiro ridasanzwe ryangiza cyangwa ryanduye hafi ya laboratoire.
Koresha imiterere, ethanol kuri pipettes, racks nintebe zo guhanagura abanduye.
Gutanga umwanya wabitswe kuri pcr yawe yose kugirango ugabanye gutandukana.
Koresha gants isukuye kuri buri ntambwe hanyuma uyisimbuze kenshi.
PCR
Kugenzura kwibanda no kweza inyandikorugero.
Isuku yintebe nibikoresho byakoreshejwe mugihe gusesengura ingero hamwe na PCR bigomba kubungabungwa. Ni ngombwa kwemeza urwego rwubuziranenge bwintangarugero mbere yo gusesengura no gutunganya.
Mubisanzwe, abasesenguzi bazitabwaho urwego rwibanze kandi rwuzuye rwicyitegererezo cya ADN.
Ihatire igipimo cyo kwinjiza 260nm / 280nm ntugomba kuba munsi ya1.8. Mugihe uburebure bwa nyuma butunganijwe hagati ya 230nm na 320nm bikoreshwa mukumenya umwanda.
Urugero, umunyutsi w'ibyuka n'ibindi bigo bya kama bigaragara ku gipimo cya 230NM. Mugihe ubwicanyi mu byitegererezo bya ADN nabyo byaragaragaye kandi bigenzurwa ku kigero cyihariye cya 320nm.
Irinde kurenza plates pcr hamwe nibicuruzwa
Nkuko byifuzwa kuyobora ibicuruzwa byinshi icyarimwe, bivamo kwanduza ibyapa bya pcr.
Kurenza ibyapa PCR nibicuruzwa bitandukanye byangiritse kandi biragoye cyane kumenya ingero.
Komeza inyandiko za aliquot pcr reagents
Gukomeza guhagarika / thaw cycle nikoreshwa rya altiquot birashobora kwangiza pcr reagents, enzymes na dntp's binyuze mumasezerano.
Buri gihe ujye wihatira gukurikirana igipimo cya aliquot cyakoreshejwe mugihe gitegura ingero zigomba gusesengurwa.
Imipaka ikwiye ikwiranye no kugenzura ibarura kandi ingano ya reagents hamwe ningero zijimye cyangwa ngo thewed.
Hitamo ubushyuhe bwiza.
Gutora no gukoresha ubushyuhe butari bwo busa nubundi buryo pcr ibisubizo birimo ikosa.
Rimwe na rimwe, reaction ntabwo igenda nkuko byari byateganijwe. Byifuzwa kugabanya ubushyuhe bukabije kugirango byorohereze neza.
Ariko, kugabanya ubushyuhe bwongera amahirwe yibyiza na primer dimers isura.
Ni ngombwa kwemeza isesengura ryo gushonga mugihe ukoresheje amasahani ya PCR nkuko ari ikimenyetso cyiza cyo guhitamo ubushyuhe buke.
Primer Gutegura Software ifasha mugushushanya, gutanga ubushyuhe bwiburyo bugabanya neza ikosa muri plates.
Ukeneye isahani nziza ya PCR?
Mugihe wagiye usuzumye aho wasangamo uwakoze wenyinePCR. Shakisha ntakindi kuko uri ahantu heza.
NezaKanda hano kugirango tundikirekubicuruzwa byose bifite ubuziranenge ku giciro kitazasenya banki.
Igihe cya nyuma: Ukwakira-30-2021